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Manuel Vera Iman Sourinejad Carmen Bouza Román Vilas Ania Pino-Querido Mohammad R. Kalbassi Paulino Martínez 《Hydrobiologia》2011,664(1):51-67
The Caspian Sea, the largest inland closed water body in the world, has numerous endemic species. The Caspian brown trout
(Salmo trutta caspius) is considered as endangered according to IUCN criteria. Information on phylogeography and genetic structure is crucial for
appropriate management of genetic resources. In spite of the huge number of studies carried out in the Salmo trutta species complex across its distribution range, very few data are available on these issues for S. trutta within the Caspian Sea. Mitochondrial (mtDNA control region) and nuclear (major ribosomal DNA internal transcribed spacer 1, ITS-1, and ten microsatellite
loci) molecular markers were used to study the phylogeography, genetic structure, and current captive breeding strategies
for reinforcement of Caspian trout in North Iranian rivers. Our results confirmed the presence of Salmo trutta caspius in this region. Phylogenetic analysis demonstrated its membership to the brown trout Danubian (DA) lineage. Genetic diversity
of Caspian brown trout in Iranian Rivers is comparable to the levels usually observed in sustainable anadromous European brown
trout populations. Microsatellite data suggested two main clusters connected by gene flow among river basins likely by anadromous
fish. No genetic differences were detected between the hatchery sample and the remaining wild populations. While the current
hatchery program has not produced detectable genetic changes in the wild populations, conservation strategies prioritizing
habitat improvement and recovering natural spawning areas for enhancing wild populations are emphasized. 相似文献
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Comparison between in vitro potency tests for Cuban hepatitis B vaccine: contribution to the standardization process. 总被引:1,自引:0,他引:1
Quality control of recombinant Hepatitis B vaccines performed by National Control Laboratories prior to marketing vaccine batches requires in vivo and or a well validated in vitro potency assays as recommended by WHO technical series. The in vitro test must also demonstrate its suitability for monitoring the consistency of the vaccine manufacturer. The aim of this study was to establish the relationship between both in vitro potency tests performed by Cuban manufacturer and National Control Laboratory for Hepatitis B vaccine and the suitability of our method for monitoring the manufacturer's test results and consistency. We also intended to contribute to the standardisation process consisting of in vitro methods for this vaccine. 相似文献