Studies of ribosomal proteins of yeast species and their hybrids gel electrophoresis and immunochemical cross-reactions

Summary The cytoplasmic ribosomal proteins (r-proteins) of seventeen yeast species of the genera Saccharomyces and Kluyveromyces were analyzed by one-dimensional gradient polyacrylamide gel electrophoresis in the presence of sodium dodecyl sulphate. The electrophoretic patterns of cytoplasmic r-proteins from different species display extensive differences in both the 40S and the 60S subunit. Relatedness of species suggested by r-protein patterns correlates well with that based on DNA/DNA homology (Bicknell and Douglas 1970). Immunochemical cross-reactions and antibiotic susceptibility tests were also used to compare different species.Analyses of r-proteins from two different interspecific hybrids showed that their ribosomes were hybrid, containing r-proteins from both parents. These findings are discussed in relation to the evolution of yeast species and the regulation of expression of r-proteins in cucaryotes.     

A dynamic model of thoracic differentiation for the control of turning in the stick insect

Leg movements of stick insects (Carausius morosus) making turns towards visual targets are examined in detail, and a dynamic model of this behaviour is proposed. Initial results suggest that front legs shape most of the body trajectory, while the middle and hind legs just follow external forces (Rosano H, Webb B, in The control of turning in real and simulated stick insects, vol. 4095, pp 145–156, 2006). However, some limitations of this explanation and dissimilarities in the turning behaviour of the insect and the model were found. A second set of behavioural experiments was made by blocking front tarsi to further investigate the active role of the other legs for the control of turning. The results indicate that it is necessary to have different roles for each pair of legs to replicate insect behaviour. We demonstrate that the rear legs actively rotate the body while the middle legs move sideways tangentially to the hind inner leg. Furthermore, we show that on average the middle inner and hind outer leg contribute to turning while the middle outer leg and hind inner leg oppose body rotation. These behavioural results are incorporated into a 3D dynamic robot simulation. We show that the simulation can now replicate more precisely the turns made by the stick insect. This work was supported by CONACYT México and the European Commission under project FP6-2003-IST2-004690 SPARK.     

The influence of temporal variation on relationships between ecosystem services

A growing literature aims to identify areas of congruence in the provision of multiple ecosystem goods and services. However, little attention has been paid to the effect that temporal variation in the provision of such services may have on understanding of these relationships. Due to a lack of temporally and spatially replicated monitoring surveys, such relationships are often assessed using data from disparate time periods. Utilising temporally replicated data for indices of freshwater quality and agricultural production we demonstrate that through time the biophysical values of ecosystem services may vary in a spatially non-uniform way. This can lead to differing conclusions being reached about the strength of relationships between services, which in turn has implications for the prioritisation of areas for management of multiple services. We present this first analysis to illustrate the effect that the use of such temporally disparate datasets may have, and to highlight the need for further research to assess under what circumstances temporal variation of this sort will have the greatest impact.     

L��essor des sciences du neurone au xxe si��cle

A great revolution in the field of neuroscience started in the 1940s and took place after the Second World War. Before the War, the fundamental questions at stake dealt with the nature of neurotransmission, chemical or electrical. The first turning point occurred in the distinguished field of neurophysiology, where a better understanding of neuronal electrogenesis and fine dissections of nervous networks followed the introduction of the new intracellular recording technique. This technique is associated with biophysics, electron microscopy and ultracentrifugation. Consequently, the chemical theory of neurotransmission is uniformly adopted with the emergence of the quantal theory and the vesicular theory. There is a new opportunity for the discovery of neurotransmitters with the help of biochemical methods. Noradrenergic and aminergic paths are described with novel histological techniques, whereas electrophysiology is used to find their functions. All disciplines work together in the study of the synaptic organisations of nerve centres and local inhibition phenomena. Neuronal sciences adopt neuropharmacology to create a novel neurochemistry. New dissection procedures, morphological network tracing tools and surgical techniques offer novel ways of intervention and manipulation. These innovations of the 1950s and 1960s are central in building a new international community unified by the American neuroscience movement.     

Next generation sequencing demonstrates association between tumor suppressor gene aberrations and poor outcome in patients with cancer

Next generation sequencing is transforming patient care by allowing physicians to customize and match treatment to their patients’ tumor alterations. Our goal was to study the association between key molecular alterations and outcome parameters. We evaluated the characteristics and outcomes (overall survival (OS), time to metastasis/recurrence, and best progression-free survival (PFS)) of 392 patients for whom next generation sequencing (182 or 236 genes) had been performed. The Kaplan-Meier method and Cox regression models were used for our analysis, and results were subjected to internal validation using a resampling method (bootstrap analysis). In a multivariable analysis (Cox regression model), the parameters that were statistically associated with a poorer overall survival were the presence of metastases at diagnosis (P = 0.014), gastrointestinal histology (P < 0.0001), PTEN (P < 0.0001), and CDKN2A alterations (P = 0.0001). The variables associated with a shorter time to metastases/recurrence were gastrointestinal histology (P = 0.004), APC (P = 0.008), PTEN (P = 0.026) and TP53 (P = 0.044) alterations. TP53 (P = 0.003) and PTEN (P = 0.034) alterations were independent predictors of a shorter best PFS. A personalized treatment approach (matching the molecular aberration with a cognate targeted drug) also correlated with a longer best PFS (P = 0.046). Our study demonstrated that, across diverse cancers, anomalies in specific tumor suppressor genes (PTEN, CDKN2A, APC, and/or TP53) were independently associated with a worse outcome, as reflected by time to metastases/recurrence, best PFS on treatment, and/or overall survival. These observations suggest that molecular diagnostic tests may provide important prognostic information in patients with cancer.