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A. A. Osmolovskiy V. G. Kreier A. V. Kurakov N. . Baranova N. S. Egorov 《Applied Biochemistry and Microbiology》2012,48(5):488-492
Natural isolates of Aspergillus ochraceus micromycetes from soil and plant remains from various regions have been screened. The isolated strains were characterized by similar cultural and morphological features and an identical nucleotide sequence in the ITS1-5,8S-ITS2 region of rDNA. The ability of the extracellular proteinases of A. ochraceus micromycetes to activate protein C of blood plasma has been established. Differences are revealed in the accumulation of proteinases activating protein C and proteinases with thrombinand plasmin-like activities in the growth dynamics of producers. 相似文献
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The effect of N1-substituted analogs of ATP on the hydrolysis of umbelliferone phosphate by Na,K-ATPase has demonstrated: analogs having a negatively charged substituent (N1-oxy- or N1-carbo-methoxy-ATP) and capable of accepting H+ induce an activation similar to that of ATP; N1-methoxy-ATP, containing an uncharged substituent, does not affect the phosphatase reaction at low concentration and inhibits it at higher concentration. It has been assumed that ATP binding to Na,K-ATPase induces formation of a hydrogen bond between the nitrogen atom at the first position of the purine base and appropriate amino acid of active centre, with a subsequent attachment of H+ to ATP, thus facilitating the transition of Na,K-ATPase from the K+- to the Na+-form. 相似文献
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Vetchinin S. S. Shevyakov A. G. Fedyukina G. N. Baranova E. V. Biketov S. F. 《Russian Journal of Bioorganic Chemistry》2018,44(6):745-754
Russian Journal of Bioorganic Chemistry - Hybridomas producing monoclonal antibodies (mABs) to the recombinant protein NS1 of the Zika virus synthesized in human embryonic kidney HEK293 (ZV293)... 相似文献
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V. S. Baranov T. Ivaschenko B. Bakay M. Aseev R. Belotserkovskaya H. Baranova P. Malet J. Perriot P. Mouraire V. N. Baskakov G. A. Savitskyi S. Gorbushin S. I. Deyneka E. Michnin A. Barchuck V. Vakharlovsky G. Pavlov V. I. Shilko T. Guembitzkaya L. Kovaleva 《Human genetics》1996,97(4):516-520
A homozygous gene deletion at the glutathioneS-transferase M1 (GSTM1) locus of genomic DNA from blood spots was studied by PCR in the group of Slavic populations from the north-western and central-eastern regions of European Russia and in patients with lung cancer (LC), other tumors (OT), endometriosis (E), alcoholic cirrhosis (AC), cystic fibrosis (CF) and chronic bronchitis (CB). The frequencies of the GSTMI 0/0 genotype were 38.8% and 67.5% for both population groups, respectively. The proportion of the GSTM1 gene deletion geno-type was estimated as significantly increased in LC (81%), OT (65%), E (81%), AC (77.3%), and in CB (73.6%) patients with symptoms of CB confirmed by X-ray but not in CB patients without X-ray evidence of disease (40.9%). A definite preponderance of GSTM1-0 homozygotes (51.1%) has been registered in CF patients of the pancreatic sufficient group with clear-cut pulmonological manifestations but not in those of the pancreatic insufficient group with predominantly intestinal or mixed clinical symptoms (41.2% and 37.5%, respectively). Earlier clinical manifestations and death before the age of 5 years are typical for GSTM1-deleted CF patients. These data support the notion that GSTM1 deletion should be considered as a convenient genetic marker for the early detection of groups at higher risk of many diseases caused by environmental and genetic factors, where manifestation depends on the lack of detoxification. High levels of GSTM1 0/0 genotypes in E patients favor the substantial contribution of certain environmental toxins in the pathogenesis of this widespread disease. 相似文献
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