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421.
Frequencies of hexa- and heptatandem tetrameric repeats in Russian slavonians from the North-Western part of the country were found to be 0.19 and 0.81, respectively, with the frequency of heterozygotes estimated as 0.31. Similar values of allelic frequencies were found for uzbek and azerbaijan populations (0.24 and 0.76; 0.22 and 0.78, respectively). These data assess the existence of genetic equilibrium of both alleles in the populations studied. Hexa TTR is present twice more often in the CF chromosomes than hepta TTR common for normal chromosomes. Absolute genetic disequilibrium of both TTR alleles was observed for CF chromosomes bearing the F508 mutation. The latter was exclusively detected in a conjunction with hexa TTR allele. Combined application of the F508 test and TTR alleles assay significantly increased the number of totally informative CF-high risk families, and thus might be highly beneficial for a subsequent prenatal diagnosis of CF. 相似文献
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The mouse liver was fixed by 12 fixator variants on the basis of glutaraldehyde, paraformaldehyde, picric acid and lysin, and the localization of albumin was studied by the indirect immunoperoxidase method. The comparison of the results shows that immersion in 8% paraformaldehyde is a preferable method of fixation. Albumin was detected on ribosomes and membranes of the nuclear envelope, the coarse endoplasmic reticulum and in the matrix of the hepatocytic cytoplasm. It is suggested that albumin synthetized is directly discharged into cytosol and then into the extracellular space. 相似文献
424.
Myasnyanko I. N. Baleeva N. S. Baranov M. S. 《Russian Journal of Bioorganic Chemistry》2022,48(3):651-654
Russian Journal of Bioorganic Chemistry - We report novel derivatives of a chromophore of the fluorescent protein Kaede, (Z)-2-(4-methoxybenzylidene)-6/8-phenylimidazo[1,2-a]pyridine-3(2H)-ones.... 相似文献
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V Kh Brikenshte?n E P Baranov B I Grinchenko L R Pitina G N Barenbo?m 《Antibiotiki》1983,28(5):346-351
Olivomycin (I) is a fluorescent probe which is highly specific with respect to DNA. Its luminescence-absorption properties were studied at various pH values, in different solvents and in the presence of Mg2+ and DNA in the solutions. The presence of 2 tautomeric forms of I was suggested on the basis of the study (forms A and B having different characteristics with respect to the absorption spectra and fluorescence). Transition between forms A and B occurred in both the basic and the excited states. The quantitative ratio of the forms depended on the character of the environment. The presence of the 2 forms of I should be considered in analysis of the drug pharmacokinetics and pharmacodynamics. 相似文献
428.
Bogdanov A. M. Gorbachev D. A. Zaitseva E. R. Smirnov A. Yu. Baleeva N. S. Baranov M. S. 《Russian Journal of Bioorganic Chemistry》2021,47(3):784-787
Russian Journal of Bioorganic Chemistry - The solvatochromic behavior of a model compound mimicking the chromophore of cyan fluorescent protein (CFP) in the spectral and time domains has been... 相似文献
429.