海岸带复合农林业系统植物种群光环境特征研究

本文对三种银杏-农作物复合模式下植物种群对光合有效辐射的削弱和截获进行了模型化分析,并探讨了不同模式下光照强度的时空分布规律。结果表明:银杏果用-叶用-豆类作物复合模式具有最好的复合光效益,光能截获率可达92%;PAR在植冠层的削弱遵循Beer-Lambert定律;植物种群的叶面积、地上部分生物量、光吸收的关系可以用Y=ax^b数学模型描述。同时,果用、材用银杏冠层有较大的透光性和光强变异系数,可以作为银杏-农作物初期经营的上层树种,但要注意冠形的调控;光强的时间变化受冠层条件和太阳高度角的双重影响。     

Biological activities of cecropin B-thanatin hybrid peptides.

Ten kinds of hybrid peptides containing the N-terminal residues of cecropin B (CB) and C-terminal of thanatin (TH) were constructed and expressed as gluthathion S-transferase (GST)-fusion proteins. Variants were screened for the better biological activity, which was paralleled with the degree of growth inhibition of the transformant cells. The hybrid CB-TH g was selected as the best one among those hybrids by in vivo monitoring method and was chemical synthesized for in vitro antimicrobial activity analysis. The hybrid peptide showed rescued activity against several test strains when compared with the truncated isoforms of TH, suggesting that the peptides with different structure and mechanism could be used as templates for hybrid peptides design.     

Immunoreactivities of AR,ERα, ERβ and aromatase in the nuptial pad of Chinese brown frog (Rana dybowskii) during pre-hibernation and the breeding period

There is a prominent local raised pad called nuptial pad on the forelimb of Chinese brown frog (Rana dybowskii), which is hypothetically concluded as an enhancement of the grip and a spreader of pheromone during the amplexus. In this study, we investigated the immunolocalization and protein expression levels of androgen receptors (AR), estrogen receptor α (ERα), ERβ and aromatase in the nuptial pad of R. dybowskii during pre-hibernation and the breeding period. Histologically, the annual development of the nuptial pad in R. dybowskii is manifested as the larger area of specialized mucous gland and the longer length of papillary epidermal projection during the breeding period. AR, ERα, ERβ and aromatase are present in the stratum granulosum, stratum spinosum, stratum basale and the secretory portion of specialized mucous glands during both periods. Western blotting results confirmed that AR, ERα and ERβ protein levels are higher during pre-hibernation than those during the breeding season. These results suggest that nuptial pad is the direct target organ of androgen and estrogen. Androgen may participate in the regulation of annual development and glandular function of nuptial pad, and estrogen may play an endocrine, autocrine or paracrine role during pre-hibernation and the breeding period.Key words: Androgen receptor, aromatase, estrogen receptor, nuptial pad, Rana dybowskii.