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171.
The complete mitochondrial genomes sequences of Asio flammeus and Asio otus and comparative analysis 总被引:2,自引:0,他引:2
SUN Yi MA Fei XIAO Bing ZHENG Junjie YUAN Xiaodong TANG Minqian WANG Li YU Yefei & Li Qingwei . College of Life Sciences Liaoning Normal University Dalian China . TaKaRa Biotechnology 《中国科学:生命科学英文版》2004,47(6):510-520
Mitochondrial DNA (mtDNA), as a model sys-tem, has been extensively used for molecular phy-logenetic and evolutionary analysis[1]. With the ad-vances in DNA sequencing technology, more andmore researchers prefer to use complete mitochondrialgenome for phylogenetic analysis[2—4]. Since the com-plete sequencing of human mtDNA in 1981 (Andersonet al., 1981)[5], 342 vertebrate mitochondrial genomeshave so far been sequenced. Up to now the completesequences of 29 avian mitochondrial genomes h… 相似文献
172.
本文根据吴征镒教授对中国种子植物属分市区类型研究结果,研制出定量化研究区域性种子植物区系的电子计算机程序,应用本程序可完成某区系的分布区类型统计分析,科属组成分析和与其它地区以共有属关系构建的相似性系数的谁知盘中计算,同时可大量节省研究人员的劳动强度和时间,对提高研究水平有一定的效果。 相似文献
173.
174.
175.
Qi-Feng Wang Yue Zhao Qiong Yi Kun-Zhi Li Yong-Xiong Yu Li-Mei Chen 《Acta Physiologiae Plantarum》2010,32(6):1209-1220
Numerous studies with transgenic plants have demonstrated that overexpression of enzymes related to organic acid metabolism under the control of CaMV 35S promoter increased organic acid exudation and Al-resistance. The synthesis of organic acids requires a large carbon skeleton supply from leaf photosynthesis. Thus, we produced transgenic tobacco overexpressing cytosolic malate dehydrogenase (MDH) cDNA from Arabidopsis thaliana (amdh) and the MDH gene from Escherichia coli (emdh), respectively, under the control of a leaf-specific light-inducible promoter (Rubisco small subunit promoter, PrbcS) in the present study. Our data indicated that an increase (120–130%) in MDH-specific activity in leaves led to an increase in malate content in the transgenic tobacco leaves and roots as well as a significant increase in root malate exudation compared with the WT plants under the acidic (pH 4.5) conditions irrespective of 300 μM Al3+ stress absence or presence. After being exposed to 25 μM Al3+ in a hydroponic solution, the transgenic plants exhibited stronger Al-tolerance than WT plants and the degree of A1 tolerance in the transgenic plants corresponded with the amount of malate secretion. When grown in an Al-stress perlite medium, the transgenic tobacco lines showed better growth than the WT plants. The results suggested that overexpression of MDH driven by the PrbcS promoter in transgenic plant leaves enhanced malate synthesis and improved Al-resistance. 相似文献
176.
Shihong Zhang Yi Wei Huimei Yu Jinliang Liu Hongyu Pan 《Russian Journal of Plant Physiology》2010,57(5):670-675
Most monocotyledons like cereals accumulate antifreeze proteins in the apoplast during cold acclimation, but it is still uncertain
whether dicotyledons do. Here we report the isolation and characterisation of a 33-kD apoplastic chitinase extracted from
the corolla of wintersweet (Chinmonanthus praecox communis L.), which was purified using successive column chromatography on Phenyl-Sepharose, DEAE-Sepharose, and CM-Sepharose. Antifreezing
activity of chitinase was confirmed in terms of the formation of bipyramidal ice crystals and high thermal-hysteresis values.
Interestingly, chitinase was also found to affect germination of fungal spores of four major plant pathogens. From these data,
we hypothesize that, under natural conditions, wintersweet as one of the overwintering dicotyledons also accumulates apoplastic
antifreeze proteins like monocotyledons. To our knowledge, this is the first report on the isolation of dicotyledon apoplastic
chitinase with high-level antifreeze and antifungal activities. 相似文献
177.
Plants have large families of proteins sharing a conserved eight-cysteine-motif (8CM) domain. The biological functions of
these proteins are largely unknown. EARLI1 is a cold responsive Arabidopsis gene that encodes a hybrid proline-rich protein (HyPRP) with a three-domain architecture:
a putative signal peptide at the N-terminus, a proline-rich domain (PRD) in the middle, and an 8CM domain at the C-terminus.
We report here that yeast cells expressing different EARLI1 genes had significantly higher rates of freezing survival than empty-vector transformed controls. Arabidopsis plants with
knocked down EARLI1 genes had an increased tendency for freezing-induced cellular damage. EARLI1-GFP fluorescence in transgenic plants and immunoblot
analyses using protoplasts suggested cell wall localization for EARLI1 proteins. Immunoblot analyses showed that EARLI1 proteins
form higher order complexes in plants, and that the PRD is a soluble and the 8CM an insoluble protein domain. We propose that
EARLI1 proteins have a bimodular architecture in which the PRD may interact with the cell wall and the 8CM domain with the
plasma membrane to protect the cells during freezing stress. 相似文献
178.
大仓鼠的核型与B染色体研究 总被引:1,自引:0,他引:1
采用骨髓细胞染色体制片法,对分布于山东济南、泰山、东北长白山和陕西西安的大仓鼠的染色体组型、G-带、C-带和银染核型进行了分析研究。济南、西安和长白山的标本的二倍体数目和核型相似,2n=28,22t+4m+XY(st,m)。泰山标本的二倍体数目为2n=28~29,即在675%的中期相中多出了一条形态最小的端着丝粒染色体,这条染色体为B染色体,可能起源于X染色体。泰山标本的A染色体组与上述3地标本相同。4地标本的G-带、C-带和银染核型相似。除B染色体外,每个端着丝粒染色体都具有着丝粒异染色质,AgNORs较恒定地出现在Nos2,4,8,9,13染色体上。也就是说大仓鼠的B染色体为C-带阴性,不携带核仁组织者。这种B染色体C-带阴性的特征在赤狐、黑家鼠和大林姬鼠朝鲜亚种中亦有报道。 相似文献
179.
钟萍蒋毅 《现代生物医学进展》2006,6(4):44-45
目的:观察A型肉毒毒素对面肌痉挛患者的痉挛程度、抑郁症状和焦虑症状的改善。方法:对58例面肌痉挛患者进行局部注射A型肉毒毒素。在治疗前后对痉挛程度改善情况进行评定以及用汉密顿焦虑量表(HAMA)、汉密顿抑郁量表(HAMD)对焦虑状态、抑郁状态进行评分,并对药物的副作用进行观察。结果:A型肉毒毒素明显改善面肌痉挛患者的痉挛程度,治疗后2周的HAMA、HA肋评分较治疗前明显下降,且差异具有统计学意义(P〈0.01)。结论:局部注射A型肉毒毒素可迅速缓解或消除面肌痉挛患者肌肉痉挛及相关的抑郁和焦虑状态,提高患者的生活质量。 相似文献
180.
脂肪来源细胞体外增殖规律及定向诱导分化研究 总被引:8,自引:0,他引:8
脂肪组织由整形外科吸脂术获得(19例,31.5±5.8岁)。酶消化法分离抽吸物中细胞,体外扩增至第10代.测定细胞生长曲线、累计倍增数目,明确其体外生长规律和增殖能力;通过对表面抗原CD29、CD105、CD106、CD166、CD49d、CD34、CD31、3G5等的检测分析脂肪来源细胞的群体组成:分别向软骨、骨、脂肪定向诱导,进一步明确该细胞群体定向分化能力。实验表明,每300ml脂肪抽吸物平均可获得5×10~7个有核细胞,体外扩增10代,平均每代倍增数目为1.59±0.224.累计倍增数目为15.53。流式细胞学及免疫细胞化学检测显示,干细胞相关抗原CD29、CD105、CD106、CD166等表达率均>60%,但与造血系相关的CD34、CD31表达率也分别达到7.3%、29.2%。ADC向软骨诱导可检测到Ⅱ型胶原表达;向成骨诱导可见矿化结节形成,并可检测到AKP、Osteonectin基因表达;向脂肪诱导可检测到PPARr2、GLU-4、Leptin基因表达,细胞内有脂滴形成。脂肪来源的细胞获得量大,体外增殖能力强,并含有具有多向分化潜能细胞,有可能作为组织构建的种子细胞。 相似文献