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11.
N. K. Mukhopadhyay S. Majumder S. K. Ghosh D. Bhattacharya S. K. Bose 《Folia microbiologica》1984,29(4):295-300
An effective method of preparation involving sonication was developed for cell-free mycobacillin synthetase fromBacillus subtilis. The enzyme showed optimum activity at a buffer concentration of 50 mM (Tris-HCl) and pH 7.5. ATP and Mg2+ which were essential for synthesis showed an optimum requirement at a ratio of 1∶1. The synthetase was markedly inhibited
by ADP whereas AMP was without any effect. ATP or ATP-generating system could not be replaced by GTP, UTP or CTP. Co2+ and Mn2+ could to some extent substitute Mg2+. Mercapto reagents inhibited the antibiotic synthesis. Exogenous addition of pantothenic acid had no effect. 相似文献
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Demonstration of auto-anti-idiotypic antibody cross-reacting with public idiotypic determinants in the serum of rye-sensitive allergic patients 总被引:5,自引:0,他引:5
R Bose D G Marsh J Duchateau A H Sehon G Delespesse 《Journal of immunology (Baltimore, Md. : 1950)》1984,133(5):2474-2478
The present study documents the presence, in the serum of one allergic individual, of auto-anti-idiotypic antibodies cross-reacting with public idiotypic determinants expressed on human IgE and IgG anti-Rye I antibodies. Sera from rye-sensitive patients were tested for specific IgG and IgE antibodies to Rye I by double antibody. The IgG fraction, isolated from the serum of a patient with a history of previous hyposensitization therapy, was repeatedly absorbed on Rye-I-Sepharose as well as on IgM- and IgG-Sepharose to remove anti-Rye I antibodies as well as any possible anti-heavy or light chain activity. This IgG fraction, named anti-idiotypic fraction (a-IdF), blocked in a dose-dependent fashion the reaction of IgG and IgE anti-Rye I antibodies with Rye I antigen. The a-IdF also blocked the binding of anti-rye antibodies to Rye I antigen in the serum of 20 unrelated allergic patients, indicating that these anti-Rye I antibodies bore public idiotypic determinants. 相似文献
13.
Role of ATP and enzyme-bound nascent peptides in the control of elongation for mycobacillin synthesis. 下载免费PDF全文
The presence of a Zn2+-dependent acid p-nitrophenyl phosphatase (EC 3.1.3.2) in bovine liver was described. The enzyme was purified to apparent homogeneity and migrates as a single band during electrophoresis on polyacrylamide gel. The enzyme requires Zn2+ ions for catalytic activity, other bivalent cations have little or no effect. The enzyme, of Mr 118,000, optimum pH 6-6.2 and pI 7.4-7.5, was inhibited by EDTA, tartrate, adenine and ATP, but not by fluoride. The common phosphate esters are poor substrates for the enzyme, which hydrolyses preferentially p-nitrophenyl phosphate and o-carboxyphenyl phosphate. The Zn2+-dependent acid p-nitrophenyl phosphatase of bovine liver was different from the high-Mr acid phosphatases previously detected in mammalian tissues. 相似文献
14.
Effect of fenitrothion (phosphorothioic acid, 0,0-dimethyl 0-4-nitro-m-tolyl ester), an organophosphorous insecticide, on
membrane permeability employing the leakage of betacyanin and electrolytes as the criteria were studied in beet root(Beta vulgaris) discs. The leakage of both betacyanin and electrolytes increased with increasing concentrations (10–150 ppm) of fenitrothion
in the incubation medium. At 0.33 mM the increase in electrolyte leakage was approximately linear for the first 6h, while
the increase in betacyanin leakage started with a lag of about 2 h. Long term incubation (24 h) showed a biphasic nature (in
the semilog plot) for the increase in betacyanin leakage, while the increase in electrolyte leakage appeared more complex.
In the control sample, the Arrhenius plots (25–50°C) of leakage showed a break at 40°C. In treated samples no break was observed,
but the slope decreased (for both electrolyte and betacyanin leakage) as compared to the respective slopes in the control
in the temperature region greater than 40°C. The results are discussed in terms of the possible effect of the insecticide
on the active transport in plant membranes 相似文献
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T. N. Khoshoo R. C. Mehra K. Bose 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1969,39(3):133-140
Summary
Ruellia tweediana and R. tuberosa are large flowered chasmogamous diploids (n=17) with normal meiosis and fertility. F
1 hybrids, successful in only one direction (R. tweediana x R. tuberosa), are vegetatively vigorous and possess 17 often heteromorphic bivalents with high degree of segregational irregularities. It is exclusively cleistogamous and completely pollen and seed sterile. Like F
1, the artificial amphidiploid (n=34) is also cleistogamous but shows preferential chromosome pairing with complete restoration of fertility. The parental chromosomes are sufficiently differentiated and cleistogamy is either genie or due to gene-cytoplasm interaction but sterility is entirely chromosomal. All floral parts excepting calyx are highly deformed. Such a deformity is associated with sterility in the F
1 but with fertility in the amphidiploid. This is perhaps the first case of origin by hybridization of a true breeding and fully fertile cleistogamous taxon from two chasmogamous species. It also shows the extent and nature of change in breeding system brought about by hybridization and/or polyploidy.The chromosome numbers in the six, out of 16, obligate cleistogamous taxa (Table 4) show that they are high polyploids. Perhaps their origin has been in the same manner as in the present case.
Zusammenfassung Ruellia tweediana und R. tuberosa sind großblütige, chasmogame Diploide (n=17) mit normaler Meiosis und Fertilität. Die F 1-Hybriden, die nur in einer Richtung gelingen (R. tweediana x R. tuberosa), sind vegetativ kräftig und besitzen häufig 17 heteromorphe Bivalente mit einem hohen Anteil an Spaltungsunregelmäßigkeiten. Die Hybride ist ausschließlich kleistogam und vollkommen pollen- und samensteril. Wie die F 1 ist auch die künstlich hergestellte Amphidiploide (n=34) kleistogam und zeigt eine präferentielle Chromosomenpaarung mit völliger Wiederherstellung der Fertilität. Die elterlichen Chromosomen sind genügend differenziert. Die Kleistogamie ist entweder genisch bedingt oder auf eine Gen-Cytoplasma-Interaktion zurückzuführen, die Sterilität ist ausschließlich durch die Chromosomen verursacht. Alle Teile der Blüte mit Ausnahme der Calyx sind stark deformiert. Bei der F 1 ist diese Deformation mit Sterilität verbunden, die amphidiploide Form ist jedoch fertil. Das ist vielleicht der erste Fall eines aus der Kreuzung zweier chasmogamer Spezies hervorgegangenen reinerbigen und voll fertilen kleistogamen Taxons. Es läßt sich auch der Umfang und die Art der durch Hybridisierung und durch Polyploidie verursachten Änderung des Zuchtsystems erkennen. Die Chromosomenzahl bei 6 von 16 obligaten kleistogamen Taxa (Tab. 4) zeigt, daß sie hochpolyploid sind. Vielleicht sind sie auf eine gleiche Weise wie im vorliegenden Falle entstanden.相似文献
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Adherence of multiple serovars of Chlamydia trachomatis to a common receptor on HeLa and McCoy cells is mediated by thermolabile protein(s) 总被引:7,自引:0,他引:7
Several aspects of the adherence of purified elementary bodies (EB) of Chlamydia trachomatis to HeLa and to McCoy cells were examined using different techniques, including an ELISA. Serovar-specific, biotinylated monoclonal antibodies were used to detect cell-bound chlamydiae. In addition, purified chlamydiae were biotinylated and their adherence properties were studied. The assays were done at 4 degrees C to exclude the energy-dependent internalization of the cell-bound EB and host-cell membrane recycling that occur at 37 degrees C. Saturation kinetics were routinely observed at 4 degrees C, and the rate of adherence remained linear for approximately 60 min. Lineweaver-Burk analysis of the kinetics data showed that adherence of any one serovar was competitively inhibited by other serovars of C. trachomatis. This competition for the same receptor on the two alternative hosts, HeLa and McCoy, was also seen when the adherence assays were done at 37 degrees C in the presence of sodium azide, an energy poison that inhibits endocytosis of cell-bound chlamydiae. Chlamydiae exposed to 56 degrees C for 5 min, or treated with low doses of trypsin, failed to exhibit competitive inhibition, having suffered considerable loss of the ability to adhere to host-cells. These data suggest that heat- and trypsin-labile chlamydial moieties participate in the adherence reaction, and that oculo-genital serovars of C. trachomatis, including that of lymphogranuloma venereum, attach to the same receptor on the host-cell membrane. 相似文献