全文获取类型
收费全文 | 179篇 |
免费 | 20篇 |
出版年
2020年 | 1篇 |
2019年 | 3篇 |
2018年 | 3篇 |
2017年 | 4篇 |
2016年 | 2篇 |
2015年 | 5篇 |
2014年 | 5篇 |
2013年 | 4篇 |
2012年 | 9篇 |
2011年 | 11篇 |
2010年 | 4篇 |
2009年 | 7篇 |
2008年 | 5篇 |
2007年 | 4篇 |
2006年 | 5篇 |
2005年 | 6篇 |
2004年 | 4篇 |
2003年 | 2篇 |
2002年 | 9篇 |
2001年 | 8篇 |
2000年 | 6篇 |
1999年 | 11篇 |
1998年 | 6篇 |
1997年 | 8篇 |
1996年 | 4篇 |
1994年 | 1篇 |
1993年 | 1篇 |
1992年 | 6篇 |
1991年 | 5篇 |
1990年 | 2篇 |
1989年 | 7篇 |
1988年 | 4篇 |
1987年 | 6篇 |
1986年 | 3篇 |
1985年 | 1篇 |
1984年 | 2篇 |
1983年 | 1篇 |
1982年 | 1篇 |
1980年 | 1篇 |
1979年 | 1篇 |
1978年 | 3篇 |
1977年 | 3篇 |
1976年 | 2篇 |
1975年 | 2篇 |
1974年 | 2篇 |
1973年 | 2篇 |
1972年 | 5篇 |
1966年 | 2篇 |
排序方式: 共有199条查询结果,搜索用时 15 毫秒
111.
Dudkiewicz M Mackiewicz P Mackiewicz D Kowalczuk M Nowicka A Polak N Smolarczyk K Banaszak J Dudek MR Cebrat S 《Bio Systems》2005,80(2):193-199
Directional mutation pressure associated with replication processes is the main cause of the asymmetry between the leading and lagging DNA strands in bacterial genomes. On the other hand, the asymmetry between sense and antisense strands of protein coding sequences is a result of both mutation and selection pressures. Thus, there are two different ways of superposition of the sense strand, on the leading or lagging strand. Besides many other implications of these two possible situations, one seems to be very important - because of the asymmetric replication-associated mutation pressure, the mutation rate of genes depends on their location. Using Monte Carlo methods, we have simulated, under experimentally determined directional mutation pressure, the divergence rate and the elimination rate of genes depending on their location in respect to the leading/lagging DNA strands in the asymmetric prokaryotic genome. We have found that the best survival strategy for the majority of genes is to sometimes switch between DNA strands. Paradoxically, this strategy results in higher substitution rates but remains in agreement with observations in bacterial genomes that such inversions are very frequent and divergence rate between homologs lying on different DNA strands is very high. 相似文献
112.
Nicolas Boutard Arkadiusz Białas Aleksandra Sabiniarz Paweł Guzik Katarzyna Banaszak Artur Biela Marcin Bień Anna Buda Barbara Bugaj Ewelina Cieluch Anna Cierpich Łukasz Dudek Hans-Michael Eggenweiler Joanna Fogt Monika Gaik Andrzej Gondela Krzysztof Jakubiec Mirek Jurzak Charles-Henry Fabritius 《Bioorganic & medicinal chemistry letters》2019,29(4):646-653
In oncology, the “Warburg effect” describes the elevated production of energy by glycolysis in cancer cells. The ubiquitous and hypoxia-induced 6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 3 (PFKFB3) plays a noteworthy role in the regulation of glycolysis by producing fructose-2,6-biphosphate (F-2,6-BP), a potent activator of the glycolysis rate-limiting phosphofructokinase PFK-1. Series of amides and sulfonamides derivatives based on a N-aryl 6-aminoquinoxaline scaffold were synthesized and tested for their inhibition of PFKFB3 in vitro in a biochemical assay as well as in HCT116 cells. The carboxamide series displayed satisfactory kinetic solubility and metabolic stability, and within this class, potent lead compounds with low nanomolar activity have been identified with a suitable profile for further in vivo evaluation. 相似文献
113.
Józef Banaszak Weronika Banaszak-Cibicka Piotr Szefer 《Journal of Insect Conservation》2014,18(4):651-656
Changes in bee fauna, such as the disappearance of certain species or increasing abundance of others, are very important. The common belief that pollinating insects are facing problems also prompts detailed studies of the bee fauna in order to track on-going changes. Assessment of the state of bee communities in particular ecosystems or ecosystem complexes within a landscape, tracing the course of trends in fauna and also predicting their future structures resulting from current changes, are only possible if sampling is carried out at an appropriate frequency, so that representative materials are obtained. The aim of the present study was to determine what sampling intensity during the growing season would enable the collection of representative materials to evaluate species diversity of bees in a study area. Repeated bee sampling at monthly intervals throughout the period of activity of bees resulted in the identification of 73 bee species, corresponding to 51.4 % of the estimated number of bee species in the study area. When samples were obtained twice a month, 93 bee species were captured, accounting for 65.5 % of the estimated number of bee species. When sampling took place nearly four times a month, 108 bee species were captured, making up 76 % of the estimated number of bee species; the materials obtained at this sampling rate may be regarded as representative. The importance of accounting for the phenology of a given animal group during a sampling effort is also emphasised. 相似文献
114.
115.
Satinder K Singh Stephen P Miller Antony Dean Leonard J Banaszak David C LaPorte 《The Journal of biological chemistry》2002,277(9):7567-7573
In Escherichia coli, the homodimeric Krebs cycle enzyme isocitrate dehydrogenase (EcIDH) is regulated by reversible phosphorylation of a sequestered active site serine. The phosphorylation cycle is catalyzed by a bifunctional protein, IDH kinase/phosphatase (IDH-K/P). To better understand the nature of the interaction between EcIDH and IDH-K/P, we have examined the ability of an IDH homologue from Bacillus subtilis (BsIDH) to serve as a substrate for the kinase and phosphatase activities. BsIDH exhibits extensive sequence and structural similarities with EcIDH, particularly around the phosphorylated serine. Our previous crystallographic analysis revealed that the active site architecture of these two proteins is almost completely conserved. We now expand the comparison to include a number of biochemical properties. Both IDHs display nearly equivalent steady-state kinetic parameters for the dehydrogenase reaction. Both proteins are also phosphorylated by IDH-K/P in the same ratio (1 mole of phosphate per mole of monomer), and this stoichiometric phosphorylation correlates with an equivalent inhibition of IDH activity. Furthermore, tandem electrospray mass spectrometry demonstrates that BsIDH, like EcIDH, is phosphorylated on the corresponding active site serine residue (Ser-104). Despite the high degree of sequence, functional, and structural congruence between these two proteins, BsIDH is surprisingly a much poorer substrate of IDH-K/P than is EcIDH, with Michaelis constants for the kinase and phosphatase activities elevated by 60- and 3,450-fold, respectively. These drastically disparate values might result from restricted access to the active site cavity and/or from the lack of a potential docking site for IDH-K/P. 相似文献
116.
M G Rossmann M J Adams M Buehner G C Ford M L Hackert A Liljas S T Rao L J Banaszak E Hill D Tsernoglou L Webb 《Journal of molecular biology》1973,76(4):533-537
A right-handed orthogonal set of axes P, Q, R has been denned coincident with the three different molecular 2-fold axes of lactate dehydrogenase. The four different symmetry-related subunits can then be color-coded red, yellow, green and blue to identify the three different subunit contacts made by any one subunit to the remaining three others. This nomenclature has been extended to soluble malate dehydrogenase and will facilitate the comparison of dehydrogenases with related ternary and quaternary structures. 相似文献
117.
118.
Molecular dynamics simulations were carried out to calculate free energy differences between the folded and unfolded states of wild type and mutant collagen model peptides. The calculated stability of the collagen models was compared with the severity of osteogenesis imperfecta. Free energy differences of Gly → Xaa (Xaa: Ser, Cys, Glu, and Asp) mutations between the wild type and the mutants at position 15 of the model peptide were 3.8, 4.2, 5.6, and 8.8 kcal/mol, respectively. The corresponding free energy differences of a second Gly mutation at the same position in different chains were, on average, 1.3, 1.5, 2.9, and 5.4 kcal/mol, respectively. Free energy simulations were also performed to estimate the relative stability between an oxidized form and a reduced form of the mutants containing two Cys residues, which indicated that the mutant of the collagen-like peptide containing an intramolecular disulfide bond was more stable than the mutant containing one Cys residue but less stable than the wild type. The calculated free energy differences between an oxidized and a reduced form of the mutants containing two Cys residues are 0.8 and 2.6 kcal/mol for the disulfide bonds between Chains A and B and between Chains A and C, respectively. 相似文献
119.
120.