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排序方式: 共有109条查询结果,搜索用时 234 毫秒
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The Drosophila melanogaster embryo has been widely utilized as a model for genetics and developmental biology due to its small size, short generation time, and large brood size. Information on embryonic metabolism during developmental progression is important for further understanding the mechanisms of Drosophila embryogenesis. Therefore, the aim of this study is to assess the changes in embryos’ metabolome that occur at different stages of the Drosophila embryonic development. Time course samples of Drosophila embryos were subjected to GC/MS-based metabolome analysis for profiling of low molecular weight hydrophilic metabolites, including sugars, amino acids, and organic acids. The results showed that the metabolic profiles of Drosophila embryo varied during the course of development and there was a strong correlation between the metabolome and different embryonic stages. Using the metabolome information, we were able to establish a prediction model for developmental stages of embryos starting from their high-resolution quantitative metabolite composition. Among the important metabolites revealed from our model, we suggest that different amino acids appear to play distinct roles in different developmental stages and an appropriate balance in trehalose-glucose ratio is crucial to supply the carbohydrate source for the development of Drosophila embryo. 相似文献
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Abstract. The fruit piercing moth, Eudocima fullonia (Clerck) (Lepidoptera: Noctuidae), whose larval host plants are vines of the family Menispermaceae in Asia, Africa and Australia, is thought to have adapted to Erythrina spp. in the Pacific and Papua New Guinea and has been designated as a separate biotype from the Australasian and African biotype. To test the hypothesis that the Pacific population of E. fullonia is a biotype, feeding trials with the host plants Tinospora homosepala Diels (Menispermaceae) and Erythrina variegata Linn. (Fabaceae) were conducted in Guam. The results indicate that the Guam population of E. fullonia is a biotype that has expanded its host range from its normal Menispermaceae plants to Erythrina species, possibly due to genetic changes and/or the presence of closely related alkaloids in both the species and paucity of menisperms. 相似文献
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Morihiro Mitsuya Kenji Kamata Makoto Bamba Hitomi Watanabe Yasuhiro Sasaki Kaori Sasaki Sumika Ohyama Hideka Hosaka Yasufumi Nagata Jun-ichi Eiki Teruyuki Nishimura 《Bioorganic & medicinal chemistry letters》2009,19(10):2718-2721
A novel class of 3,6-disubstituted 2-pyridinecarboxamide derivatives was designed based on X-ray analysis of the 2-aminobenzamide lead class. Subsequent chemical modification led to the discovery of potent GK activators which eliminate potential toxicity concerns associated with an aniline group of the lead structure. Compound 7 demonstrated glucose lowering effect in a rat OGTT model. 相似文献
26.
Tomoharu Iino Yasuhiro Sasaki Makoto Bamba Morihiro Mitsuya Akio Ohno Kenji Kamata Hideka Hosaka Hiroko Maruki Mayumi Futamura Riki Yoshimoto Sumika Ohyama Kaori Sasaki Masato Chiba Norikazu Ohtake Yasufumi Nagata Jun-ichi Eiki Teruyuki Nishimura 《Bioorganic & medicinal chemistry letters》2009,19(19):5531-5538
We describe design, syntheses and structure–activity relationships of a novel class of 4,6-disubstituted quinazoline glucokinase activators. Prototype quinazoline leads (4 and 5) were designed based on the X-ray analyses of the previous 2-aminobenzamide lead classes. Modifications of the quinazoline leads led to the identification of a potent GK activator (21d). 相似文献
27.
Karseno Kazuo Harada Takeshi Bamba Susilaningsih Dwi Aparat Mahakhant Tomoaki Yoshikawa Kazumasa Hirata 《Biotechnology letters》2009,31(7):999-1003
During growth of the freshwater cyanobacteria, Oscillatoria sp. BTCC/A0004, and Scytonema sp. TISTR 8208, a pink pigment is released into the growth medium. The pigment from each source had a molecular weight of
approximately 250 kDa and had adsorption maxima at 560 and 620 nm. These results suggest that pink pigment is a phycoerythrin-like
protein. It inhibited the growth of green algae, Chlorella fusca and Chlamydomonas reinhardtii, but not other cyanobacteria or true bacteria. The concentration at which growth inhibition 50% occurred was 0.5, 6 and more
than 10 mg ml−1, respectively. 相似文献
28.
Tightly winding structure of sequential model peptide for repeated helical region in Samia cynthia ricini silk fibroin studied with solid-state NMR 总被引:1,自引:0,他引:1
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Nakazawa Y Bamba M Nishio S Asakura T 《Protein science : a publication of the Protein Society》2003,12(4):666-671
There are many kinds of silks from silkworms and spiders with different structures and properties, and thus, silks are suitable to study the structure-property relationship of fibrous proteins. Silk fibroin from a wild silkworm, Samia cynthia ricini, mainly consists of the repeated similar sequences by about 100 times where there are alternative appearances of the polyalanine (Ala)(12-13) region and the Gly-rich region. In this paper, a sequential model peptide, GGAGGGYGGDGG(A)(12)GGAGDGYGAG, which is a typical sequence of the silk fibroin, was synthesized, and the atomic-level conformations of Gly residues at the N- and C-terminal ends of the polyalanine region were determined as well as that of the central Ala residue using (13)C 2D spin diffusion solid-state nuclear magnetic resonance (NMR) under off-magic angle spinning. In the model peptide with alpha-helical conformation, the torsion angle of the central Ala residue, the 19th Ala, was determined to be (phi, psi) = (-60 degrees, -50 degrees ), which was a typical alpha-helical structure, but the torsion angles of two Gly residues, the 12th and 25th Gly residues, which are located at the N- and C-terminal ends of the polyalanine region, were determined to be (phi, psi) = (-70 degrees, -30 degrees ) and (phi, psi) = (-70 degrees, -20 degrees ), respectively. Thus, it was observed that the turns at both ends of polyalanine with alpha-helix conformation in the model peptide are tightly wound. 相似文献
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T Kohsaka H Takahara H Sasada T Kawarasaki K Bamba J Masaki S Tagami 《Journal of reproduction and fertility》1992,95(2):397-408
Light-microscope immunocytochemistry using the peroxidase-antiperoxidase technique and a polyclonal rabbit antiserum raised against purified porcine relaxin showed that cytoplasmic immunostaining for relaxin could be visualized in the epithelial cells of the seminal vesicle. No relaxin immunoreactivity was seen in the testis, epididymis, ductus deferens, prostate or bulbo-urethral gland. A ten times higher concentration of porcine relaxin antiserum was necessary to achieve immunostaining in the seminal vesicle comparable to that in the corpora lutea of pregnant sows. Ultrastructural examination showed that the epithelial cells of the boar seminal vesicle resembled typical protein-secreting cells with prominent rough endoplasmic reticulum and well-developed Golgi apparatus. The most striking feature of these cells was the accumulation of granules with a limiting membrane, which ranged from 200 to 600 nm in diameter and contained flocculent material of moderate electron density. Electron-microscope immunocytochemistry using the protein A-gold technique and relaxin antiserum demonstrated that the granules were the only intracellular organelles that showed immunoreactivity for relaxin. These results indicate that a relaxin-like substance is present in boar seminal vesicles and that the subcellular site of its localization is the granules, suggesting that the seminal vesicle produces and stores a relaxin-like substance, but that it is present at much lower concentrations than in the corpora lutea of pregnant sows. 相似文献