全文获取类型
收费全文 | 599篇 |
免费 | 133篇 |
国内免费 | 1篇 |
出版年
2021年 | 7篇 |
2018年 | 6篇 |
2016年 | 7篇 |
2015年 | 22篇 |
2014年 | 24篇 |
2013年 | 29篇 |
2012年 | 30篇 |
2011年 | 30篇 |
2010年 | 22篇 |
2009年 | 29篇 |
2008年 | 25篇 |
2007年 | 27篇 |
2006年 | 25篇 |
2005年 | 17篇 |
2004年 | 21篇 |
2003年 | 13篇 |
2002年 | 16篇 |
2001年 | 19篇 |
2000年 | 19篇 |
1999年 | 11篇 |
1998年 | 15篇 |
1997年 | 6篇 |
1996年 | 12篇 |
1995年 | 8篇 |
1994年 | 14篇 |
1993年 | 7篇 |
1992年 | 8篇 |
1991年 | 12篇 |
1990年 | 11篇 |
1989年 | 17篇 |
1988年 | 51篇 |
1987年 | 13篇 |
1986年 | 9篇 |
1985年 | 8篇 |
1984年 | 5篇 |
1983年 | 7篇 |
1981年 | 6篇 |
1980年 | 6篇 |
1978年 | 5篇 |
1977年 | 9篇 |
1975年 | 12篇 |
1974年 | 8篇 |
1973年 | 5篇 |
1972年 | 6篇 |
1971年 | 6篇 |
1970年 | 12篇 |
1969年 | 6篇 |
1968年 | 5篇 |
1967年 | 9篇 |
1966年 | 5篇 |
排序方式: 共有733条查询结果,搜索用时 447 毫秒
51.
Thomas M. Newsome Guy‐Anthony Ballard Christopher R. Dickman Peter J. S. Fleming Remy van de Ven 《Ecography》2013,36(8):914-925
The idea that groups of individuals may develop around resource patches led to the formulation of the Resource Dispersion Hypothesis (RDH). We tested the predictions of the RDH, within a quasi‐experimental framework, using Australia’s largest terrestrial predator, the dingo Canis lupus dingo. Average dingo group sizes were higher in areas with abundant focal food sources around two mine sites compared with those in more distant areas. This supports the notion that resource richness favours larger group size, consistent with the RDH. Irrespective of season or sex, average home range estimates and daily activity for dingoes around the mine sites were significantly less than for dingoes that lived well away. Assuming that a territory is the defended part of the home range and that territory size is correlated with home range size, consistent with the RDH, the spatial dispersion of food patches therefore determined territory size for dingoes in our study. However, although sample size was small, some dingoes that accessed the supplementary food resource at the mines also spent a large proportion of their time away, suggesting a breakdown of territorial defence around the focal food resource. This, in combination with the large variation in home range size among dingoes that accessed the same supplementary food resource, limits the predictive capabilities of the RDH for this species. We hypothesize that constraints on exclusive home range occupancy will arise if a surfeit of food resources (in excess of requirements for homeostasis) is available in a small area, and that this will have further effects on access to mates and social structure. We present a conceptual model of facultative territorial defence where focal resources are available to demonstrate our findings. 相似文献
52.
53.
The K+/H+ antiporter LeNHX2 increases salt tolerance by improving K+ homeostasis in transgenic tomato 总被引:1,自引:0,他引:1
RAÚL HUERTAS LOURDES RUBIO OLIVIER CAGNAC MARÍA JESÚS GARCÍA‐SÁNCHEZ JUAN DE DIOS ALCHÉ KEES VENEMA JOSÉ ANTONIO FERNÁNDEZ MARÍA PILAR RODRÍGUEZ‐ROSALES 《Plant, cell & environment》2013,36(12):2135-2149
The endosomal LeNHX2 ion transporter exchanges H+ with K+ and, to lesser extent, Na+. Here, we investigated the response to NaCl supply and K+ deprivation in transgenic tomato (Solanum lycopersicum L.) overexpressing LeNHX2 and show that transformed tomato plants grew better in saline conditions than untransformed controls, whereas in the absence of K+ the opposite was found. Analysis of mineral composition showed a higher K+ content in roots, shoots and xylem sap of transgenic plants and no differences in Na+ content between transgenic and untransformed plants grown either in the presence or the absence of 120 mm NaCl. Transgenic plants showed higher Na+/H+ and, above all, K+/H+ transport activity in root intracellular membrane vesicles. Under K+ limiting conditions, transgenic plants enhanced root expression of the high‐affinity K+ uptake system HAK5 compared to untransformed controls. Furthermore, tomato overexpressing LeNHX2 showed twofold higher K+ depletion rates and half cytosolic K+ activity than untransformed controls. Under NaCl stress, transgenic plants showed higher uptake velocity for K+ and lower cytosolic K+ activity than untransformed plants. These results indicate the fundamental role of K+ homeostasis in the better performance of LeNHX2 overexpressing tomato under NaCl stress. 相似文献
54.
55.
B Forbes L Szabo R C Baxter F J Ballard J C Wallace 《Biochemical and biophysical research communications》1988,157(1):196-202
Competitive binding experiments with insulin-like growth factor (IGF)-1, IGF-2 and des-(1-3)-IGF-1 have confirmed the interpretation based on limited amino-terminal sequence analysis that at least three types of IGF binding protein occur. In addition to the acid stable subunit of the large serum binding protein which exhibits des-(1-3)-IGF-1 binding only slightly less than IGF-1, the small IGF binding proteins can be separated into two classes based on differences in des-(1-3)-IGF-1 and IGF-2 binding potencies. 相似文献
56.
Yu A Ballard L Smillie L Pearlstone J Foguel D Silva J Jonas A Jonas J 《Biochimica et biophysica acta》1999,1431(1):53-63
The N-domain of troponin C (residues 1-90) regulates muscle contraction through conformational changes induced by Ca2+ binding. A mutant form of the isolated domain of avian troponin C (F29W) has been used in previous studies to observe conformational changes that occur upon Ca2+ binding, and pressure and temperature changes. Here we set out to determine whether the point mutation itself has any effects on the protein structure and its stability to pressure and temperature in the absence of Ca2+. Molecular dynamics simulations of the wild-type and mutant protein structures suggested that both structures are identical except in the main chain and the loop I region near the mutation site. Also, the simulations proposed that an additional cavity had been created in the core of the mutant protein. To determine whether such a cavity would affect the behavior of the protein when subjected to high pressures and temperatures, we performed 1H-NMR experiments at 300, 400, and 500 MHz on the wild-type and F29W mutant forms of the chicken N-domain troponin C in the absence of Ca2+. We found that the mutant protein at 5 kbar pressures had a destabilized beta-sheet between the Ca2+-binding loops, an altered environment near Phe-26, and reduced local motions of Phe-26 and Phe-75 in the core of the protein, probably due to a higher compressibility of the mutant. Under the same pressure conditions, the wild-type domain exhibited little change. Furthermore, the hydrophobic core of the mutant protein denatured at temperatures above 47 degrees C, while the wild-type was resistant to denaturation up to 56 degrees C. This suggests that the partially exposed surface mutation (F29W) significantly destabilizes the N-domain of troponin C by altering the packing and dynamics of the hydrophobic core. 相似文献
57.
Ectoparasites were collected from chemically immobilized swift foxes (Vulpes velox) in the Texas Panhandle (USA). Three species of fleas (Pulex irritans, Dactylopsylla percernis, and Euhoplopsyllus affinis) and one species of tick (Ixodes sculptus) were found. Pulex irritans was the only abundant ectoparasite; it occurred on all 23 foxes brushed in 1999-2000 and all but one of 34 hosts examined in 2000-01. Otherwise, this swift fox population had a depauperate ectoparasite fauna; the remainder of the ectoparasites only occurred on a few (< or =5%) of the hosts. Because of previous taxonomic confusion between P. irritans and the closely related P. simulans, the zoogeographic distribution of these two species in many areas of western North America needs to be verified. Apparently, only the human flea P. irritans occurs on wild canids in the Texas Panhandle. However, there are previous records of P. simulans on other carnivores in western and central Texas. Some of these specimens were reexamined, and their identifications were reconfirmed. Also, the recent literature on the controversial taxonomic status of these two flea species is reviewed. The male terminal aedeagal sexual apparatus is the only means currently available to separate P. irritans and P. simulans. 相似文献
58.
Linking phylogenetics with population genetics to reconstruct the geographic origin of a species 总被引:10,自引:0,他引:10
Reconstructing ancestral geographic origins is critical for understanding the long-term evolution of a species. Bayesian methods have been proposed to test biogeographic hypotheses while accommodating uncertainty in phylogenetic reconstruction. However, the problem that certain taxa may have a disproportionate influence on conclusions has not been addressed. Here, we infer the geographic origin of Drosophila simulans using 2,014 bp of the period locus from 63 lines collected from 18 countries. We also analyze two previously published datasets, alcohol dehydrogenase related and NADH:ubiquinone reductase 75 kDa subunit precursor. Phylogenetic inferences of all three loci support Madagascar as the geographic origin of D. simulans. Our phylogenetic conclusions are robust to taxon resampling and to the potentially confounding effects of recombination. To test our phylogenetically derived hypothesis we develop a randomization test of the population genetics prediction that sequences from the geographic origin should contain more genetic polymorphism than those from derived populations. We find that the Madagascar population has elevated genetic polymorphism relative to non-Madagascar sequences. These data are corroborated by mitochondrial DNA sequence data. 相似文献
59.
60.