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91.
Asmita A. Prabhune Suchitra A. Baliga Asha Chandwadkar Hephzibah SivaRaman 《Biotechnology Techniques》1996,10(8):589-594
Summary Ethanolic fermentation of glucose by Saccharomyces cerevisiae was accelerated in presence of Silicalite only when the yeast was highly flocculent, the zeolite lowering floc size. With the sucrose and molasses, however, fermentation was accelerated even when the yeasts have low flocculence, the zeolite enhancing invertase activity of the yeast cells. 相似文献
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Effect of nucleoside triphosphate and magnesium ion concentration on the stability and function of rat liver polysomes in vitro 总被引:1,自引:1,他引:0 下载免费PDF全文
The effects of different concentrations of ATP, GTP, UTP and CTP on polysome stability and function in a cell-free protein-synthesizing system prepared from rat liver were studied. Increasing the concentration of ATP in the incubation medium to 15mm resulted in progressive disaggregation of the polysomes; at ATP concentrations above 2mm their capacity to incorporate amino acids into peptide chains diminished. The same disaggregation phenomenon could be produced by incubating polysomes in a buffered medium containing 5mm-Mg(2+) and increasing concentrations of ATP. Although the disaggregating action of ATP could be prevented by increasing Mg(2+) concentration, the amino acid incorporation in the cell-free protein-synthesizing system remained impaired. The effects of different concentrations of GTP, UTP and CTP on polysome stability were similar to those of ATP. Increasing the concentrations of each nucleoside triphosphate also inhibited the hydrolysis of GTP in the cell-free protein-synthesizing system. 相似文献
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Bhatt J Subbaiah G Kambli S Shah B Patel M Saxena A Baliga A Nigam S Parekh H Yadav G 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2007,852(1-2):374-381
A high throughput and sensitive liquid chromatography-tandem mass spectrometry (LC-MS/MS) method has been developed and validated for the estimation of bisoprolol in human plasma using multiplexing technique (two HPLC units connected to one MS). Bisoprolol was extracted from human plasma using solid-phase extraction technique using metoprolol as internal standard. A Betabasic 8 column provided chromatographic separation of analytes followed by detection with mass spectrometry. The mass transition ion-pair was followed as m/z 326.2-->116.1 for bisoprolol and m/z 268.2-->191.0 for metoprolol. The method involves a simple multiplexing, rapid solid-phase extraction, simple isocratic chromatography conditions and mass spectrometric detection which enable detection at sub-nanogram levels. The proposed method has been validated for a linear range of 0.5-70.0 ng/mL with correlation coefficient > or =0.9991. The precision and accuracy were within 10% for intra-HPLC runs and inter-HPLC runs. The overall recoveries for bisoprolol and metoprolol were 93.89% and 77.65%, respectively. Total MS run time was 0.90 min only. The developed method was applied for the determination of pharmacokinetic parameters of bisoprolol following a single oral administration of a 10mg bisoprolol tablet in 18 healthy male volunteers. 相似文献
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Selenium and GPx-1 overexpression protect mammalian cells against UV-induced DNA damage 总被引:2,自引:0,他引:2
Baliga MS Wang H Zhuo P Schwartz JL Diamond AM 《Biological trace element research》2007,115(3):227-242
Supplementation of the culture media of human MCF-7 breast carcinoma cells or mouse fibroblasts with low levels of selenium (30 nM) provided as sodium selenite was shown to protect these cells from ultraviolet (UV)-induced chromosome damage, as quantified by micronucleus assay. Selenium supplementation was also effective in reducing UV-induced gene mutations as measured in the lacI shuttle vector model. Protection was dependent on functional BRCA1 activity, a protein implicated in breast cancer risk and DNA damage repair. In addition, overexpression of GPx-1, a selenoprotein with antioxidant activity, also attenuated UV induced micronuclei formation in the absence of selenium supplementation. Combining selenium supplementation with GPx-1 overexpression further reduced UV-induced micronucleus frequency. These data provide evidence that the benefits of selenium supplementation might be either through the prevention or repair of DNA damage, and they implicate at least one selenoprotein (GPx-1) in the process. 相似文献
95.
A refined genome‐scale reconstruction of Chlamydomonas metabolism provides a platform for systems‐level analyses 下载免费PDF全文
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Gaggle Tool Creator (GTC) is a web application which provides access to public annotation, interaction, orthology, and genomic
data for hundreds of organisms, and enables instant analysis of the data using many popular web-based and desktop applications. 相似文献