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Lysine vasopressin (5.2 and 10.4 μ M) failed to significantly alter the spontaneous release of (3H)-noradrenaline from hippocampal slices in vitro or the release observed after stimulation with potassium chloride (15 and 25 mM). Uptake of (3H)-noradrenaline by synaptosomes prepared from hippocampal tissue was not significantly changed by incubation with vasopressin (5.2 and 10.4 μ M). Our results fail to support the hypothesis, derived from behavioural and in vivo biochemical studies, that vasopressin modulates the activity of noradrenergic nerve terminals in this part of the limbic system. 相似文献
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GROWTH AND DEVELOPMENT OF NESTLING HEN HARRIERS 总被引:1,自引:0,他引:1
Nestling Hen Harriers Circus cyaneus in Orkney were weighed and measured during two nesting seasons. The 501 weights and 390 longest primary measurements produce a composite record of growth for this species. The general shape of both weight and primary growth curves is sigmoid. The primary feather growth can be measured at age seven and eight days after hatching and is less variable as an index of growth than is weight. The nestling period varies usually from 30 to 36 days, and age at first flight depends on the ratio of primary length to weight. Males usually fly before their heavier female siblings. The asynchronous hatching produces a size-rank between nestling Hen Harriers. The significance of the size-rank to growth and mortality is discussed. Sexual dimorphism develops at the nestling stage. Weights and measurements of males and females at different ages are tabulated. Asynchronous hatching, and the influence of latitude differences on growth rate are briefly discussed. 相似文献
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Cocaine potentiates HIV-1 replication in human peripheral blood mononuclear cell cocultures. Involvement of transforming growth factor-beta 总被引:7,自引:0,他引:7
P K Peterson G Gekker C C Chao R Schut T W Molitor H H Balfour 《Journal of immunology (Baltimore, Md. : 1950)》1991,146(1):81-84
Cocaine use is an important high risk behavior in the AIDS epidemic. In this study, we tested the hypothesis that cocaine potentiates the replication of HIV-1 in human PBMC. A coculture system was used in which PBMC from healthy donors were incubated in the absence (control) or presence of cocaine before activation with PHA. Cocultures were then constituted with PBMC infected with a clinical isolate of HIV-1. HIV-1 replication, which was assessed by the measurement of HIV-1 p24 antigen levels in coculture supernatants, was significantly increased in a dose-dependent manner by cocaine with maximal stimulation at a concentration of 10(-9) M (965 +/- 196 vs 303 +/- 80 pg p24 Ag/ml in control cocultures). Antibodies to transforming growth factor-beta (TGF-beta) blocked cocaine-enhanced HIV-1 replication and purified TGF-beta stimulated viral replication in a manner similar to that observed with cocaine. Augmentation of HIV-1 replication by TGF-beta was maximal at a concentration of 0.01 ng/ml; however, viral proliferation appeared to be inhibited by concentrations of TGF-beta of 1 ng/ml or greater. Taken together, these results indicate that cocaine augments the replication of HIV-1 in PHA-activated PBMC via a mechanism that appears to involve TGF-beta. 相似文献
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