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51.
Picceri Giada Giusi Leonardi Pamela Iotti Mirco Gallo Michele Baldi Franco Zambonelli Alessandra Amicucci Antonella Vallorani Luciana Piccoli Giovanni Ciccimarra Giovanni Arshakyan Marselina Burattini Sabrina Falcieri Elisabetta Chiarantini Laura 《Applied microbiology and biotechnology》2018,102(3):1429-1441
Applied Microbiology and Biotechnology - Iron exopolysaccharide nanoparticles were biogenerated during ferric citrate fermentation by Klebsiella oxytoca DSM 29614. Before investigating their... 相似文献
52.
Hagai Levine Hideo Mohri Anders Ekbom Liliana Ramos Geoff Parker Eduardo Roldan Luca Jovine Sabine Koelle Anna Lindstrand Simone Immler Sharon Mortimer David Mortimer Gerhard van der Horst Sumio Ishijima Natalie Aneck-Hahn Elisabetta Baldi Roelof Menkveld Susan A Rothmann Aleksander Giwercman Yvonne Giwercman Mats Holmberg Ulrik Kvist Lars Björndahl Rebecka Holmberg Stefan Arver John Flanagan Joël R Drevet 《Andrologie》2018,28(1):13
On the occasion of the XIIIth International Symposium on Spermatology held from 9 to 13 May 2018 in Stockholm (Sweden), participants (guest speakers and audience) collectively felt the need to make a public statement on the general issue of male reproductive health. Our intention is to raise awareness of what we believe is a neglected area of research despite alarming situations around the world. The disclosure strategy desired by the co-authors is to bring it to the attention of the greatest number partly by considering co-publication in the various periodicals dealing with Reproductive Biology and Andrology. BaCA’s editorial office accepted this mission and found it natural that our periodical, the official journal of the French Andrology Society (SALF), should carry this message. 相似文献
53.
Marcos Vinicius Antunes de Lemos Elisa Peripolli Mariana Piatto Berton Fabiele Loise Braga Feitosa Bianca Ferreira Olivieri Nedenia Bonvino Stafuzza Rafael Lara Tonussi Sabrina Kluska Hermenegildo Lucas Justino Chiaia Lenise Mueller Adrielli Mathias Ferrinho Angelica Simone Cravo Prereira Henrique Nunes de Oliveira Lucia Galvão de Albuquerque Fernando Baldi 《Journal of applied genetics》2018,59(2):203-223
The aim of this study was to analyze the association between the copy number variation regions (CNVRs) and fatty acid profile phenotypes for saturated (SFA), monosaturated (MUFA), polyunsaturated (PUFA), ω6 and ω3 fatty acids, PUFA/SFA and ω6/ω3 ratios, as well as for their sums, in Nellore cattle (Bos primigenius indicus). A total of 963 males were finished in feedlot and slaughtered with approximately 2 years of age. Animals were genotyped with the BovineHD BeadChip (Illumina Inc., San Diego, CA, USA). The copy number variation (CNV) detection was performed using the PennCNV algorithm. Log R ratio (LRR) and allele B frequency (BAF) were used to estimate the CNVs. The association analyses were done using the CNVRuler software and applying a logistic regression model. The phenotype was adjusted using a linear model considering the fixed effects of contemporary group and the animal age at slaughter. The fatty acid profile was analyzed on samples of longissimus thoracis muscle using gas chromatography with a 100-m capillary column. For the association analysis, the adjusted phenotypic values were considered for the traits, while the data was adjusted for the effects of the farm and year of birth, management groups at birth, weaning, and superannuation. A total of 186 CNVRs were significant for SFA (43), MUFA (42), PUFA (66), and omega fatty acid (35) groups, totaling 278 known genes. On the basis of the results, several genes were associated with several fatty acids of different saturations. Olfactory receptor genes were associated with C12:0, C14:0, and C18:0 fatty acids. The SAMD8 and BSCL2 genes, both related to lipid metabolic process, were associated with C12:0. The RAPGEF6 gene was found to be associated with C18:2 cis-9 cis-12 n-6, and its function is related to regulation of GTPase activity. Among the results, we highlighted the olfactory receptor activity (GO:0004984), G-protein-coupled receptor activity (GO:0004930), potassium:proton antiporter activity (GO:0015386), sodium:proton antiporter activity (GO:0015385), and odorant-binding (GO:0005549) molecular functions. A large number of genes associated with fatty acid profile within the CNVRs were identified in this study. These findings must contribute to better elucidate the genetic mechanism underlying the fatty acid profile of intramuscular fat in Nellore cattle. 相似文献
54.
Xiao Shen Danijel Dojcinovic Lucia Baldi David L. Hacker Immanuel F. Luescher Florian M. Wurm 《Biotechnology letters》2018,40(1):85-92
Objectives
To investigate the effects of operational process conditions on expression of MHC class II protein from a stable Drosophila S2 cell line.Results
When the Drosophila S2 cells were grown in vented orbitally shaken TubeSpin bioreactor 600 containers, cell growth was improved three-fold and the yield of recombinant major histocompatibility (MHC) class II protein (HLA-DR12xHis) increased four-fold over the levels observed for the same cells cultivated in roller bottles (RB) without vented caps. Culturing in RB with vented caps while increasing the rotation speed from 6 rpm to 18 rpm also improved cell growth five-fold and protein productivity three-fold which is comparable to the levels observed in the orbitally shaken containers. Protein activity was found to be almost identical between the two vessel systems tested.Conclusions
Optimized cell culture conditions and a more efficient vessel type can enhance gas transfer and mixing and lead to substantial improvement of recombinant product yields from S2 cells.55.
Franco Baldi Salvatore Daniele Michele Gallo Stefano Paganelli Dario Battistel Oreste Piccolo Claudia Faleri Anna Maria Puglia Giuseppe Gallo 《Biometals》2016,29(2):321-331
Silver nanoparticles (AgNPs), embedded into a specific exopolysaccharide (EPS), were produced by Klebsiella oxytoca DSM 29614 by adding AgNO3 to the cultures during exponential growth phase. In particular, under aerobic or anaerobic conditions, two types of silver nanoparticles, named AgNPs-EPSaer and the AgNPs-EPSanaer, were produced respectively. The effects on bacterial cells was demonstrated by using Escherichia coli K12 and Kocuria rhizophila ATCC 9341 (ex Micrococcus luteus) as Gram-negative and Gram-positive tester strains, respectively. The best antimicrobial activity was observed for AgNPs-EPSaer, in terms of minimum inhibitory concentrations and minimum bactericidal concentrations. Observations by transmission electron microscopy showed that the cell morphology of both tester strains changed during the exposition to AgNPs-EPSaer. In particular, an electron-dense wrapped filament was observed in E. coli cytoplasm after 3 h of AgNPs-EPSaer exposition, apparently due to silver accumulation in DNA, and both E. coli and K. rhizophila cells were lysed after 18 h of exposure to AgNPs-EPSaer. The DNA breakage in E. coli cells was confirmed by the comparison of 3-D fluorescence spectra fingerprints of DNA. Finally the accumulation of silver on DNA of E. coli was confirmed directly by a significant Ag+ release from DNA, using the scanning electrochemical microscopy and the voltammetric determinations. 相似文献
56.
Antonio De Luca Ruggero De Maria Alfonso Baldi Rossana Trotta Francesco Facchiano Antonio Giordano Roberto Testi Gianluigi Condorelli 《Journal of cellular biochemistry》1997,64(4):579-585
Recent evidence suggested a role for the cell cycle dependent kinases cdc2 and cdk2 in apoptosis. An important mechanism by which many cell types could undergo apoptosis is through the activation of the Fas molecule on the cell membrane. To investigate whether Fas-induced cell death activated cdc2 and cdk2 kinases inappropriately, the human T lymphoma cells HUT-78, which express a high copy number of Fas, and two other previously characterized subclones of the same cell line which express mutant, cell death-deficient dominant-negative forms of Fas, were Fas-challenged and the changes in cdc2 and cdk2 kinase activity monitored. In both wild-type and Fas-mutated HUT-78 cells, apoptosis was associated simultaneously with decreased cdc2 and increased cdk2 activity. This association suggested that changes in cdc2 and cdk2 kinase activity are secondary events in cell death mediated by Fas. J. Cell. Biochem. 64:579–585. © 1997 Wiley-Liss, Inc. 相似文献
57.
Baldi Paolo Moser Mirko Brilli Matteo Vrhovsek Urska Pindo Massimo Si-Ammour Azeddine 《Planta》2017,245(5):1021-1035
Planta - A coordinated regulation of different branches of the flavonoid pathway was highlighted that may contribute to elucidate the role of this important class of compounds during the early... 相似文献
58.
HtrA1-dependent proteolysis of TGF-beta controls both neuronal maturation and developmental survival
Launay S Maubert E Lebeurrier N Tennstaedt A Campioni M Docagne F Gabriel C Dauphinot L Potier MC Ehrmann M Baldi A Vivien D 《Cell death and differentiation》2008,15(9):1408-1416
Transforming growth factor-beta (TGF-beta) signalling controls a number of cerebral functions and dysfunctions including synaptogenesis, amyloid-beta accumulation, apoptosis and excitotoxicity. Using cultured cortical neurons prepared from either wild type or transgenic mice overexpressing a TGF-beta-responsive luciferase reporter gene (SBE-Luc), we demonstrated a progressive loss of TGF-beta signalling during neuronal maturation and survival. Moreover, we showed that neurons exhibit increasing amounts of the serine protease HtrA1 (high temperature responsive antigen 1) and corresponding cleavage products during both in vitro neuronal maturation and brain development. In parallel of its ability to promote degradation of TGF-beta1, we demonstrated that blockage of the proteolytic activity of HtrA1 leads to a restoration of TGF-beta signalling, subsequent overexpression of the serpin type -1 plasminogen activator inhibitor (PAI-1) and neuronal death. Altogether, we propose that the balance between HtrA1 and TGF-beta could be one of the critical events controlling both neuronal maturation and developmental survival. 相似文献
59.
60.
Chiara Pecorini Serge Delpal Sandrine Truchet Fabienne Le Provost Antonella Baldi Michèle Ollivier-Bousquet 《Cell and tissue research》2009,338(2):241-255
Lactoferrin is synthesized by glandular epithelial cells and neutrophils and is also present on both sides of the mammary
epithelium. We have studied the origin of lactoferrin detected in the various compartments of mouse mammary tissue. As revealed
by immunogold electron microscopy, lactoferrin is present in mammary epithelial cells and in the basal region of the epithelium,
associated with connective tissue and stroma cells at all physiological stages studied. A perturbation of protein synthesis
or transport after in vitro treatment with cycloheximide or brefeldin A does not abrogate lactoferrin labelling in the basal
region of the epithelium. The expression of lactoferrin has also been observed in the fat pads of mammary glands from mice
surgically depleted of epithelial cells. The sealing of one teat for 24 h is accompanied by an increase in both the number
of stroma cells and the labelling of myoepithelial cells. Thus, the lactoferrin present in the interstitial space of the mouse
mammary epithelium originates in part from stroma cells. Possible roles of lactoferrin at the basal side of the mammary epithelium
are discussed. 相似文献