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351.
Ten known, viz. Criconema (Nothocriconemella) mutabile (Taylor, 1936) Raski & Luc, 1985, Neolobocriconema aberrans (Jairajpuri & Siddiqi, 1963) Andrássy, 1979, Macroposthonia sphaerocephala (Taylor, 1936) De Grisse & Loof, 1965, Nothocriconemoides basili (Jairajpuri, 1963) Loof & De Grisse, 1974, Hemicriconemoides cocophillus Loos, 1949, H. litchi Edward & Misra, 1963, H. mangiferae Siddiqi, 1961, Aulosphora indica (Siddiqi, 1961) Siddiqi, 1980, Paratylenchus obtusicaudatus Raski, 1975, and Tylenchulus semipenetrans Cobb, 1913, plus three new species of criconematids are recorded from Haryana state, India. Larvae of N. aberrans and N. basili are described for the first time. The main distinguishing features of the new species are as follows. M. paronostris n. sp.: R=117–128, R V=7–9, spear=55–62 μm, submedian lobes present, larvae with smooth annules; Criconemoides punicus n. sp.: R=75–85, R V=5–7, spear=73–81 μm, larvae with crenate annules: Hemicycliophora siddiqii n. sp.: R=185–198, R V=33–42, spear=57–59 μm, one incisure plus longitudinal lines on the cuticle. ac]19860702 相似文献
352.
Summary The nitrogen availability index determined as nitric acid potential gave significant correlations with the yield of bajra,
wheat and rice crops in experiments conducted on alluvial soils of Delhi at the IARI Farm. The coefficient of correlation
was also the highest when data from similar experiments on wheat on alluvial, grey brown, sierozem and medium black soils
from different places in India were pooled. It was found that the nitric acid potential seems to be independent of soil type
and is also applicable to various agroclimatic conditions. 相似文献
353.
The importance of cellular polyamine (PA) levels and the ratio of putrescine (Put) to spermidine (Spd) for plant regeneration ability via somatic embryogenesis in several commercially grown indica rice varieties is reported here. The genotypes namely NDR-624, IR-20, IR-36, BJ-1 (having Put:Spd ratio2.3) showed superior plant regeneration while KL, PB-1 and TN-1 (having Put:Spd ratio3.8) showed moderate plant regeneration ability. The genotypes namely HS, Bindli, DV-85, ACB-72, IR-64 and IR-72 (having Put:Spd ratio5.0) showed poor plant regeneration ability. In contrast KH-7 (Put:Spd ratio10.0) showed no response at all. Favorable modification of cellular PA titers and their Put:Spd ratio by the addition of exogenous PAs (Put, Spd) or their biosynthesis inhibitor, difluoromethylarginine (DFMA) led to the induction/promotion of plant regeneration in poorly responding genotypes. These results showed a close relationship between cellular PA levels and their Put:Spd ratio with in vitro morphogenetic capacity in indica rice and suggest that the cellular PAs and Put:Spd ratios are important determinants (biomarkers) of plant regeneration ability in indica rice, and the improvement/induction of plant regeneration in morphogenetically poor and recalcitrant species could be achieved by modulating PA metabolism. 相似文献
354.
355.
AAM Coelho-Castelo AP Trombone RS Rosada RR Santos Jr VLD Bonato A Sartori CL Silva 《Genetic vaccines and therapy》2006,4(1):1-10
In order to assess a new strategy of DNA vaccine for a more complete understanding of its action in immune response, it is important to determine the in vivo biodistribution fate and antigen expression. In previous studies, our group focused on the prophylactic and therapeutic use of a plasmid DNA encoding the Mycobacterium leprae 65-kDa heat shock protein (Hsp65) and achieved an efficient immune response induction as well as protection against virulent M. tuberculosis challenge. In the present study, we examined in vivo tissue distribution of naked DNA-Hsp65 vaccine, the Hsp65 message, genome integration and methylation status of plasmid DNA. The DNA-Hsp65 was detectable in several tissue types, indicating that DNA-Hsp65 disseminates widely throughout the body. The biodistribution was dose-dependent. In contrast, RT-PCR detected the Hsp65 message for at least 15 days in muscle or liver tissue from immunized mice. We also analyzed the methylation status and integration of the injected plasmid DNA into the host cellular genome. The bacterial methylation pattern persisted for at least 6 months, indicating that the plasmid DNA-Hsp65 does not replicate in mammalian tissue, and Southern blot analysis showed that plasmid DNA was not integrated. These results have important implications for the use of DNA-Hsp65 vaccine in a clinical setting and open new perspectives for DNA vaccines and new considerations about the inoculation site and delivery system. 相似文献
356.
G. Pichan R. K. Gauttam O. S. Tomar A. C. Bajaj 《International journal of biometeorology》1988,32(3):176-180
Physical work capacity (PWC180) was assessed with different levels of hypohydration in 25 heat-acclimatized male volunteers in hot dry (45°C DB, 30% RH) and hot humid (39°C DB, 60% RH) conditions equated to a heat stress level of 34°C on the WBGT scale. Heat acclimatization was carried out by exposing the subjects for 8 consecutive days in a climatic chamber with moderate work for two 50 min work cycles and 10 min intervening rest pauses. Acclimatization resulted in significant decreases in heart rate (27 bpm), oral temperature (0.8°C), mean skin temperature (1.2°C) and a significant increase in sweating rate (120 g h–1 m–2). Day-to-day variations in body hypohydration levels during heat acclimatization were not significantly different, although water intake was found to increase significantly from day 3 onwards when the subjects were in ad lib water intake state. The heat acclimatized subjects were then hypohydrated to varying degrees, viz. 1%, 2% and 3% body weight deficit, with moderate work in heat in the climatic chamber and after successful recovery from the effects of thermal stress and exercise; their physical work capacity was assessed individually. Physical work capacity was found to decrease significantly with hypohydration as compared to controls. The decrease was of the order of 9%, 11% and 22% in the hot dry condition and 6%, 8% and 20% in the hot humid condition with hypohydration levels of 1%, 2% and 3% respectively. The decrease was more pronounced during 3% hypohydration level under both heat stress conditions. This decrease was in spite of significant increases in maximal ventilation. However, the PWC180 under the two heat stress conditions, when compared, did not reveal any significant difference. It was concluded that the heat stress vehicle did not adversely affect the physical work capacity. On the other hand, the decreases in physical work capacity were found to be closely related to the primary hypohydration level in heat-acclimatized tropical subjects.Abbreviations
WBGT
wet bulb globe temperature
-
bam
beats per minute
-
YSI
Yellow Springs Instrument
-
EKG
electrocardiogram 相似文献
357.
358.
Hadi N Yehia Rockford K Draper Carole Mikoryak Erin Kate Walker Pooja Bajaj Inga H Musselman Meredith C Daigrepont Gregg R Dieckmann Paul Pantano 《Journal of nanobiotechnology》2007,5(1):8-17
This work concerns exposing cultured human epithelial-like HeLa cells to single-walled carbon nanotubes (SWNTs) dispersed
in cell culture media supplemented with serum. First, the as-received CoMoCAT SWNT-containing powder was characterized using
scanning electron microscopy and thermal gravimetric analyses. Characterizations of the purified dispersions, termed DM-SWNTs,
involved atomic force microscopy, inductively coupled plasma – mass spectrometry, and absorption and Raman spectroscopies.
Confocal microRaman spectroscopy was used to demonstrate that DM-SWNTs were taken up by HeLa cells in a time- and temperature-dependent
fashion. Transmission electron microscopy revealed SWNT-like material in intracellular vacuoles. The morphologies and growth
rates of HeLa cells exposed to DM-SWNTs were statistically similar to control cells over the course of 4 d. Finally, flow
cytometry was used to show that the fluorescence from MitoSOX™ Red, a selective indicator of superoxide in mitochondria, was
statistically similar in both control cells and cells incubated in DM-SWNTs. The combined results indicate that under our
sample preparation protocols and assay conditions, CoMoCAT DM-SWNT dispersions are not inherently cytotoxic to HeLa cells.
We conclude with recommendations for improving the accuracy and comparability of carbon nanotube (CNT) cytotoxicity reports. 相似文献
359.