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Jinhee Lee Ahyun Son Paul Kim Soon Bin Kwon Ji Eun Yu Gyoonhee Han Baik L. Seong 《Biotechnology and bioengineering》2019,116(3):i-i
Transglutaminase (TGase) induces the cross-linking of proteins by catalyzing an acyl transfer reaction. TGase is a zymogen, activated by the removal of its pro-region. Because the pro-region is crucial for folding and inhibition of the TGase activity, the recombinant expression of the mature TGase (mTGase) without the pro-region, usually results in inactive inclusion bodies or low protein yield. Here, Streptomyces netropsis TGase was fused with Escherichia coli lysyl-tRNA synthetase (LysRS), as a module with chaperoning activity in an RNA dependent manner (chaperna). The TGase activity from purified fusion protein induced via the removal of LysRS by tev protease in vitro. Moreover, active mTGase was produced in E. coli via an intracellular cleavage system, wherein LysRS-mTGase was cleaved by the coexpressed tev protease in vivo. The results suggest that LysRS essentially mimics pro-region, which exerts a dual function—folding of TGase into active conformation and keeping it as dormant state—in an RNA-dependent manner. Thus, trans-acting RNAs, prompt the cis-acting chaperone function of LysRS, while being mechanistically similar to the intramolecular chaperone function of the pro-region. These results could be implemented and extended for the folding of “difficult-to-express” recombinant proteins, by harnessing the chaperna function. 相似文献
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Oyunbileg Erdenetsogt Jun Nuri Sung Moon-hee Yoon Daewui Baik Inkyung 《Sleep and biological rhythms》2018,16(4):399-404
Sleep and Biological Rhythms - To evaluate the effects of poly-gamma-glutamic acid (γ-PGA) administration on inflammatory and metabolic biomarkers in sleep-restricted rats, we conducted an... 相似文献
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One/third of microregenerants derived from the clone of a single cell of the Pottia intermedia gametophyte survived and formed protonemal mats on a surface of the Knop agar with 0.5 μM HgCl2. A high survival rate (in percents) argues for the epigenetic character of adaptation. Epigenetic adaptation of the clone
is accompanied by increasing of a cell number in leaves, by increasing of the peroxidase activity and by the intensification
of the activity zone of the peroxidase isoform, a molecular weight of which was 66 kDa, and by the appearance of two isoforms
of this enzyme on the electrophoregram. The significantly weak increasing of the peroxidase activity was manifested in the
regenerants from the Pottia clone, which demonstrated an adaptation to 0.2 μM HgCl2 on a mass scale [8]; the epigenetically adapted regenerants grown in a metal free media were different from the physiologically
adapted regenerants only by the intensification of the zone of activity of the isoform with a molecular weight of 66 kDa.
This could be the reason to consider the epigenetic adaptation to the elevated mercury concentration as an intensified epigenecopy
of the modification and to point out to the similarity of the mechanisms of both types of adaptation. 相似文献
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