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71.
Miao Yan Xin Li Jinbao Gu Guojun Gao Ziyu Wu Peng Xue 《Journal of biochemical and molecular toxicology》2023,37(3):e23265
Prostate cancer (PCa) is a challenging issue for men's health worldwide due to its uncontrolled proliferation and high metastatic potential. Increasing evidence has supported plant extracts and natural plant derivatives as promising antitumor therapy with less toxic side effects. Yuanhuacine is an active component isolated from Daphne genkwa and can effectively suppress the tumorigenesis of several cancers. However, its role in PCa remains unclear. In this study, yuanhuacine dose-dependently inhibited the proliferation and induced apoptosis of PCa cells. Moreover, yuanhuacine also restrained the invasion and migration of PCa cells. Mechanically, yuanhuacine decreased the ubiquitination and degradation of p53 protein, and ultimately increased p53 levels, which was regulated by inhibiting the phosphorylation and total protein levels of mouse double minute 2 (MDM2). Moreover, elevation of MDM2 reversed the suppressive efficacy of yuanhuacine in PCa cell viability, invasion, and migration. The network pharmacologic and bioinformatics analysis confirmed that MDM2 might be a common target of D. genkwa and LINC00665. Furthermore, yuanhuacine inhibited LINC00665 expression. Upregulation of LINC00665 reversed yuanhuacine-mediated inhibition in MDM2 protein expression and suppressed p53 levels by enhancing its ubiquitination in yuanhuacine-treated cells. Importantly, the inhibitory effects of yuanhuacine on cell viability and metastatic potential were offset after LINC00665 elevation. Together, the current findings highlight that yuanhuacine may possess tumor-suppressive efficacy by inhibiting LINC00665-mediated MDM2/p53 ubiquitination signaling. Therefore, this study indicates that yuanhuacine may be a promising candidate for the treatment of PCa. 相似文献
72.
73.
Béatrice Drouet Luis Garcia Dominique Simon-Chazottes Marie Geneviève Mattei Jean-Louis Guénet Arnold Schwartz Gyula Varadi Martine Pinçon-Raymond 《Mammalian genome》1993,4(9):499-503
Using both chromosomal in situ hybridization and molecular techniques, we report the genetic localization of the gene coding for the alpha 1 subunit of the skeletal slow Ca2+ current channel/DHP receptor gene (Cchl1a3) on human Chromosome (Chr) 1 (1q31–1q32 region) and on mouse Chr 1 region (F-G). On the basis of single-strand conformation polymorphism (SSCP-PCR) analysis in an interspecific backcross, we have determined that the Cchl1a3=mdg (muscular dysgenesis) locus is very closely linked to the myogenin (Myog) locus. 相似文献
74.
Chair of Committee for Mouse Chromosome 19 相似文献
75.
76.
In this paper, the author studies the quartic bioecology differential system:Here n and q are positive constants, l、m and p are constants. The author also proves some theorems of existence and uniqueness of limit cycles of this differential system. 相似文献
77.
长年饲养在高温(28—30℃)环境中雌性中华大蟾蜍,它们的卵母细胞可以长足,但经激素处理时,生发泡不破裂,仅显示成熟过程早期阶段的变化。值得注意的是,在孕酮刺激后的高温卵卵质中,出观了一种能诱发低温卵恢复减数分裂的物质,称作为“依赖冬眠因子的促成熟物质”(HF-MPS)。HF-MPS 与MPF 有不少相似之处,如孕酮处理后,它们在卵质中出现的时间相仿,它们的形成均不依赖于转录水平,而是依赖于翻译水平的蛋白质合成活动;但亦存在不同之处,如MPF 诱发低温卵GVBD 时程不受温度影响,而HF-MPS 在10℃环境中,诱发低温卵GVBD 的时间明显延缓;MPF 不仅能诱发低温卵GVBD,而且同样能诱发高温卵GVBD,然而,HF-MPS 只能诱发低温卵GVBD。由此表明,MPF 和HF-MPS 似乎是截然不同的两类活性蛋白质。高温卵缺少低温诱发产生的“冬眠因子”,所以不能恢复cdc 2基因的转录活动,不能实现MPF 自身催化扩增作用,不能保证孕酮处理后的卵母细胞完成正常成熟的全过程变化。足见,低温是中华大蟾蜍卵母细胞恢复减数分裂过程中的必要条件,是导致中华大蟾蜍现有区域分布的内在原因之一。 相似文献
78.
K. Schuster-Gossler D. Simon-Chazottes J. -L. Guénet J. Zachgo A. Gossler 《Mammalian genome》1996,7(1):20-24
We have produced a transgenic mouse line, Gtl2
lacZ
(Gene trap locus 2), that carries an insertional mutation with a dominant modified pattern of inheritance:heterozygous Gtl2
lacZ
mice that inherited the transgene from the father show a proportionate dwarfism phenotype, whereas the penetrance and expressivity
of the phenotype is strongly reduced in Gtl2
lacZ
mice that inherited the transgene from the mother. On a mixed genetic background this pattern of inheritance was reversible
upon transmission of the transgene through the germ line of the opposite sex. On a predominantly 129/Sv genetic background,
however, transgene passage through the female germ line modified the transgene effect, such that the penetrance of the mutation
was drastically reduced and the phenotype was no longer obvious after subsequent male germ line transmission. Expression of
the transgene, however, was neither affected by genetic background nor by parental legacy. Gtl2
lacZ
maps to mouse Chromosome 12 in a region that displays imprinting effects associated with maternal and paternal disomy. Our
results suggest that the transgene insertion in Gtl2
lacZ
mice affects an endogenous gene(s) required for fetal and postnatal growth and that this gene(s) is predominantly paternally
expressed.
Received: 30 May 1995 / Accepted: 7 August 1995 相似文献
79.
80.
Garðar Guðmundsson 《Vegetation History and Archaeobotany》1996,5(1-2):13-23
Lyme-grass (Elymus arenarius L.) was harvested and processed for human consumption along the southern coast of Iceland until the beginning of this century.
The origin of this exploitation is not known, but lyme-grass may have been gathered for this purpose already in the Viking
Age. The system of processing lyme-grass is complex, and special implements and facilities are needed. The method is similar
to traditional processing methods for cereals. Extensive research on Icelandic ethnographic observations was undertaken to
establish the exact methods and sequence of operations that was involved. On the basis of this, a set of archaeological experiments
was performed to test the viability of the processing techniques outlined in the written records. A model describing the harvesting
and processing of lyme-grass is presented. 相似文献