RNAi knockdown of Oryza sativa root meander curling gene led to altered root development and coiling which were mediated by jasmonic acid signalling in rice

Jasmonic acid (JA) is a well-known defence hormone, but its biological function and mechanism in rice root development are less understood. Here, we describe a JA-induced putative receptor-like protein (OsRLK, AAL87185) functioning in root development in rice. RNA in situ hybridization revealed that the gene was expressed largely in roots, and a fusion protein showed its localization on the plasma membrane. The primary roots in RNAi transgenic rice plants meandered and curled more easily than wild-type (WT) roots under JA treatment. Thus, this gene was renamed Oryza sativa root meander curling (OsRMC). The transgenic primary roots were shorter, the number of adventitious roots increased and the number of lateral roots decreased as compared to the WT. As well, the second sheath was reduced in length. Growth of both primary roots and second sheaths was sensitive to JA treatment. No significant change of JA level appeared in the roots between the transgenic rice line and WT. Expression of RSOsPR10, involved in the JA signalling pathway, was induced in transgenic rice. Western blotting revealed OsRMC induced by JA. Our results suggest that OsRMC of the DUF26 subfamily involved in JA signal transduction mediates root development and negatively regulates root curling in rice.     

基于高通量测序技术对山羊盲肠细菌多样性的分析

【背景】由于反刍动物特殊的生理结构,以往研究者主要集中对其瘤胃微生物的结构与组成进行了大量研究,严重忽略了盲肠微生物在营养物质消化和肠道健康方面发挥的重要作用。【目的】采用高通量测序技术分析山羊盲肠细菌的多样性及菌群结构。【方法】选用12只10月龄健康母羊,其平均体重为20.70±1.60kg,饲喂20d后,采集每只山羊的盲肠内容物,提取微生物总DNA,用细菌通用引物对细菌16S rRNA基因的高可变区进行PCR扩增,利用Illumina MiSeq平台对扩增子进行高通量测序,并用QIIME等软件对测序序列进行生物信息学分析。【结果】山羊盲肠微生物测序共获得813 496条有效序列与6 883个OTU,并且稀释曲线和Coverage指数反映此次测序结果比较全面的覆盖了山羊盲肠微生物群落。α多样性和β多样性分析表明,山羊个体之间盲肠微生物的多样性存在差异。在门水平,各样品的优势菌门均为厚壁菌门(Firmicutes)和拟杆菌门(Bacteroidetes);属水平,核心菌群由梭菌属(Clostridium)、瘤胃球菌属(Ruminococcus)和6个未分类的细菌组成。PICRUSt基因预测表明,山羊盲肠微生物以代谢功能为主,主要包括:碳水化合物代谢、氨基酸代谢、能量代谢和脂质代谢等。【结论】山羊盲肠与瘤胃细菌的多样性存在显著差异,与粪便微生物组成相似;与单胃动物相比,两者盲肠微生物的组成既有共性,也存在差异。     

31例非特异性阴道炎病人阴道微生物群的研究

本文对31例非特异性阴道炎病人和31例正常人阴道微生物群进行定性、定量分析.结果表明,阴道炎病人的阴道乳杆菌的平均浓度明显低于正常人,而肠杆菌科、葡萄球菌、棒状杆菌、类杆菌属、支原体却明显增多,阴道炎病人未发现特异性病原体,菌群失调可能是其发病因素。乳杆菌为阴道正常优势菌,对改善阴道的微环境,防止条件致病菌引起的内源性感染具有重要的生理作用。     

钙调素化学发光标记的研究

用发光物质N-氨基丁基N-乙基异鲁米诺(ABEI),采用碳二亚胺缩合法,成功地标记了动物及植物钙调素(CaM),标记率为[ABEI]/[CaM]≈0.8-0.9.对ABEI标记CaM的反应条件、结合物的质量和保存时间进行了观察.此标记物稳定,-20℃可保存10个月以上,适用于化学发光免疫法测定生物样品中CaM含量.     

Antibodies to the alpha-subunit of insulin receptor from eggs of immunized hens

Simple methods for the generation, purification, and assay of antibodies to the alpha-subunit of insulin receptor from eggs of immunized hens have been described. Chicken antibodies against the alpha-subunit inhibit insulin binding to the receptor and stimulate glucose oxidation as well as autophosphorylation of the beta-subunit. Thus the properties of chicken antibodies are very similar to those of antibodies found in human autoimmune diseases and different from rabbit antibodies obtained against the same antigen.     

利用絮凝进行微藻采收的研究进展

微藻可生产不饱和脂肪酸及色素等多种高附加值产品,同时也可用来生产可再生清洁能源如生物柴油等,具有良好的应用前景。但是,目前微藻细胞的采收成本高居不下,已成为限制微藻生物技术大规模应用的重要因素之一。与其他方法相比,絮凝采收成本低、操作简便,是很有应用前景的采收方法。本文综述了国内外利用化学絮凝、物理絮凝及生物絮凝等方法对不同微藻细胞进行采收的研究,重点对生物絮凝方法进行了总结。利用微生物絮凝剂及微藻细胞的自絮凝进行微藻生物量的回收,是微藻采收技术中环境友好、低成本和行之有效的新方法之一。     

Novel quantitative trait loci (QTL) for Fusarium head blight resistance in wheat cultivar Chokwang

Fusarium head blight (FHB) is one of the most destructive diseases in wheat. This study was to identify new quantitative trait loci (QTL) for FHB resistance and the molecular markers closely linked to the QTL in wheat cultivar Chokwang. The primers of 612 simple sequence repeats (SSRs) and 12 target-region-amplified polymorphism (TRAP) marker were analyzed between resistant (Chokwang) and susceptible (Clark) parents. One hundred and seventy-two polymorphic markers were used to screen a population of 79 recombinant inbred lines (RILs) derived from the cross of Chokwang and Clark. One major QTL, Qfhb.ksu-5DL1, was identified on chromosome 5DL. The SSR marker Xbarc 239 was mapped in the QTL region, and also physically located to the bin of 5DL1-0.60-0.74 by using Chinese Spring deletion lines. Another QTL Qfhb.ksu-4BL1was linked to SSR Xbarc 1096 and tentatively mapped on 4BL. A QTL on 3BS, Qfhb.ksu-3BS1, was also detected with marginal significance in this population. Different marker alleles for these QTL were detected between Chokwang and Sumai 3 and its derivatives. These results suggested that Chokwang contains new QTL for FHB resistance that are different from those in Sumai 3. Pyramiding resistance QTL from various sources may enhance FHB resistance in wheat cultivars.     

电针对帕金森病模型小鼠黑质铁染色细胞和铁蛋白表达的影响

目的探讨电针防治帕金森病的作用机制。方法采用1-甲基-4-苯基1,2,3,6四氢吡啶腹腔注射建立帕金森病小鼠模型,利用组织化学技术以及免疫组织化学技术观察电针对帕金森病小鼠脑黑质铁染色细胞和铁蛋白表达的影响。结果模型组1周、2周、4周小鼠黑质铁染色阳性细胞的数量较正常组明显增多,染色强度也明显增强（P〈0．001;P〈0．01;P〈0．001）,电针在3个存活期内均可明显减少帕金森病小鼠黑质铁阳性细胞的数量和染色强度（P〈0．001;P〈0．05;P〈0．001）;模型组1周、2周黑质部位铁蛋白的表达与正常组相比都有不同程度下降（P〈0．01;P〈0．001）,而电针组2周帕金森病小鼠黑质铁蛋白的表达与模型组相比显著增加（P〈0．01）。结论电针可以通过降低脑内铁的含量,同时增加铁蛋白的表达,从而提高帕金森病模型小鼠脑抗氧化能力,达到保护黑质多巴胺能神经元的目的。