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51.
Bahram Jafar-Mohammadi Christopher J. Groves Katharine R. Owen Timothy M. Frayling Andrew T. Hattersley Mark I. McCarthy Anna L. Gloyn 《PloS one》2009,4(8)
Background
There is considerable interest in the hypothesis that low frequency, intermediate penetrance variants contribute to the proportion of Type 2 Diabetes (T2D) susceptibility not attributable to the common variants uncovered through genome-wide association approaches. Genes previously implicated in monogenic and multifactorial forms of diabetes are obvious candidates in this respect. In this study, we focussed on exons 8–10 of the HNF1A gene since rare, penetrant mutations in these exons (which are only transcribed in selected HNF1A isoforms) are associated with a later age of diagnosis of Maturity onset diabetes of the young (MODY) than mutations in exons 1–7. The age of diagnosis in the subgroup of HNF1A-MODY individuals with exon 8–10 mutations overlaps with that of early multifactorial T2D, and we set out to test the hypothesis that these exons might also harbour low-frequency coding variants of intermediate penetrance that contribute to risk of multifactorial T2D.Methodology and Principal Findings
We performed targeted capillary resequencing of HNF1A exons 8–10 in 591 European T2D subjects enriched for genetic aetiology on the basis of an early age of diagnosis (≤45 years) and/or family history of T2D (≥1 affected sibling). PCR products were sequenced and compared to the published HNF1A sequence. We identified several variants (rs735396 [IVS9−24T>C], rs1169304 [IVS8+29T>C], c.1768+44C>T [IVS9+44C>T] and rs61953349 [c.1545G>A, p.T515T] but no novel non-synonymous coding variants were detected.Conclusions and Significance
We conclude that low frequency, nonsynonymous coding variants in the terminal exons of HNF1A are unlikely to contribute to T2D-susceptibility in European samples. Nevertheless, the rationale for seeking low-frequency causal variants in genes known to contain rare, penetrant mutations remains strong and should motivate efforts to screen other genes in a similar fashion. 相似文献52.
Ahmad Separham Samad Ghaffari Bahram Sohrabi Naser Aslanabadi Mozhgan Hadavi Bavil Hasanali Lotfollahi 《Andrologie》2017,27(1):14
Background
Low level of testosterone may be associated with cardiovascular diseases in men, as some evidence suggests a protective role for testosterone in cardiovascular system. Little is known about the possible role of serum testosterone in response to reperfusion therapy in ST-elevation myocardial infarction (STEMI) and its relationship with ST-segment recovery. The present study was conducted to evaluate the association of serum testosterone levels with ST-segment resolution following primary percutaneous coronary intervention (PPCI) in male patients with acute STEMI.Methods
Forty-eight men (mean age 54.55 ± 12.20) with STEMI undergoing PPCI were enrolled prospectively. Single-lead ST segment resolution in the lead with maximum baseline ST-elevation was measured and patients were divided into two groups according to the degree of ST-segment resolution: complete (> or =50%) or incomplete (<50%). The basic and demographic data of all patients, their left ventricular ejection fraction (LVEF) and laboratory findings including serum levels of free testosterone and cardiac enzymes were recorded along with angiographic finding and baseline TIMI (Thrombolysis in Myocardial Infarction) flow and also in-hospital complications and then these variables were compared between two groups.Results
A complete ST-resolution (≥50%) was observed in 72.9% of the patients. The serum levels of free testosterone (P = 0.04), peak cardiac troponin (P = 0.03) were significantly higher and hs-CRP (P = 0.02) were lower in patients with complete ST-resolution compared to those with incomplete ST-resolution. In-hospital complications were observed in 31.2% of patients. The patients with a lower baseline TIMI flow (P = 0.03) and those who developed complications (P = 0.04) had lower levels of free testosterone. A significant positive correlation was observed between the left ventricular function and serum levels of free testosterone (P = 0.01 and r = +0.362).Conclusion
This study suggests that in men with STEMI undergoing PPCI, higher serum levels of testosterone are associated with a better reperfusion response, fewer complications and a better left ventricular function.53.
Background
Rapid identification of bacteria can play an important role at the point of care, evaluating the health of the ecosystem, and discovering spatiotemporal distributions of a bacterial community. We introduce a method for rapid identification of bacteria in live cell assays based on cargo delivery of a nucleic acid sequence and demonstrate how a mixed culture can be differentiated using a simple microfluidic system.Methods
C60 Buckyballs are functionalized with nucleic acid sequences and a fluorescent reporter to show that a diversity of microorganisms can be detected and identified in live cell assays. The nucleic acid complexes include an RNA detector, targeting a species-specific sequence in the 16S rRNA, and a complementary DNA with an attached fluorescent reporter. As a result, each bacterium can be detected and visualized at a specific emission frequency through fluorescence microscopy.Results
The C60 probe complexes can detect and identify a diversity of microorganisms that include gram-position and negative bacteria, yeast, and fungi. More specifically, nucleic-acid probes are designed to identify mixed cultures of Bacillus subtilis and Streptococcus sanguinis, or Bacillus subtilis and Pseudomonas aeruginosa. The efficiency, cross talk, and accuracy for the C60 probe complexes are reported. Finally, to demonstrate that mixed cultures can be separated, a microfluidic system is designed that connects a single source-well to multiple sinks wells, where chemo-attractants are placed in the sink wells. The microfluidic system allows for differentiating a mixed culture.Conclusions
The technology allows profiling of bacteria composition, at a very low cost, for field studies and point of care.54.
Azam Sadat Hosseini Alhashemi Abdolreza R. Karbassi Bahram Hassanzadeh Kiabi Seyed Masoud Monavari Seyed Mohammad Bagher Nabavi Mohammad Sadegh Sekhavatjou 《Biological trace element research》2011,142(3):500-516
Present study investigates relationships between total and bioaccessibility of trace elements (Cd, Co, Cr, Cu, Mn, NI, Pb,
V, and Zn) concentrations in sediment and their bioaccumulation in species in Shadegan wetland in southwest of Iran. Bioavailability
factor (BAF) and translocation factor (TF) were calculated in plants and trophic transfer factor (TTF) was determined in bird
species. For this purpose, sampling of sediments, aquatic plants including Phragmites australis, Typha australis, Scripus maritimus and two bird species encircling Porphyrio porphyrio and globally threatened Marmaronetta angustirostris were carried out during winter 2009. Result of chemical analysis show that bioaccessibility concentrations of Mn (8.31 mg/kg),
V (1.33 mg/kg), and Pb (1.03 mg/kg) are higher than other metals. The uptake trend of trace elements in plant decreases as
root > stem > leaf. Accumulation levels of trace elements in different tissues of P. porphyrio and M. angustirostris are almost identical and considerable. Accumulation and toxicity of Cd in birds is more than plants. In addition, BAF of
V, Pb, and Cr indicates high accumulation by plants and great pollution rate in the area of study. In S. maritimus TF for Mn, Cu, Pb, and V are high whereas in T. australis, Cu and Pb posses the highest TF. Also Cr, Co, Mn, Ni, and Zn have higher TF from stem to leaf than root to stem in P. australis. Finally, TTFs were compared in various bird species. 相似文献
55.
Atri MS Saboury AA Moosavi-Movahedi AA Goliaei B Sefidbakht Y Alijanvand HH Sharifzadeh A Niasari-Naslaji A 《Journal of biomolecular structure & dynamics》2011,28(6):919-928
α-Lactalbumin α-La), together with oleic acid can be converted to a complex, which kills tumor cells selectively. Cytotoxic α-La -oleic acid and α-La -linoleic acid complexes were generated by adding fatty acid to camel holo α-La at 60 ° C (referred to as La-OA-60 and La-LA-60 state, respectively). Structural properties of these complexes were studied and compared to the camel α-La. The experimental results show that linoleic acid induces α-La partial unfolding but oleic acid does not change the protein structure significantly. Also the stability of La-OA-60 and La-LA-60 toward thermal denaturation was measured. The order of temperature at the transition midpoint is as follows: La-LA-60 < La-OA-60 < α-La. La-OA-60 complex inhibited tubulin polymerization in vitro. Although the structures of La-OA-60 and La-LA-60 were different, these two complexes had similar cytotoxic effect to DU145 human prostate cancer cells. Samples of La-OA-60 that have been renatured after denaturation lost the specific biological activity toward tumor cells. 相似文献
56.
Uhlén M Björling E Agaton C Szigyarto CA Amini B Andersen E Andersson AC Angelidou P Asplund A Asplund C Berglund L Bergström K Brumer H Cerjan D Ekström M Elobeid A Eriksson C Fagerberg L Falk R Fall J Forsberg M Björklund MG Gumbel K Halimi A Hallin I Hamsten C Hansson M Hedhammar M Hercules G Kampf C Larsson K Lindskog M Lodewyckx W Lund J Lundeberg J Magnusson K Malm E Nilsson P Odling J Oksvold P Olsson I Oster E Ottosson J Paavilainen L Persson A Rimini R Rockberg J Runeson M Sivertsson A 《Molecular & cellular proteomics : MCP》2005,4(12):1920-1932
Antibody-based proteomics provides a powerful approach for the functional study of the human proteome involving the systematic generation of protein-specific affinity reagents. We used this strategy to construct a comprehensive, antibody-based protein atlas for expression and localization profiles in 48 normal human tissues and 20 different cancers. Here we report a new publicly available database containing, in the first version, approximately 400,000 high resolution images corresponding to more than 700 antibodies toward human proteins. Each image has been annotated by a certified pathologist to provide a knowledge base for functional studies and to allow queries about protein profiles in normal and disease tissues. Our results suggest it should be possible to extend this analysis to the majority of all human proteins thus providing a valuable tool for medical and biological research. 相似文献
57.
The generally positive relationship between biodiversity of groups of directly or indirectly interacting organisms is one of the most important ecological concepts (Gaston, 2000 Nature, 405 , 220–227; Scherber C, Eisenhauer N, Weisser WW et al., 2010 Nature, 468 , 553–556). In a recent issue of Molecular Ecology, Gao C, Shi N‐N, Liu Y‐X et al. (2013: 22 , 3403–3414) reported that the richness of plants and ectomycorrhizal fungi is positively correlated both at local and at global scales. Here, we challenge these findings by re‐analysis of data and ascribe the reported results to sampling effect and poor data compilation. 相似文献
58.
Cristina L. Vázquez Thomas R. Lerner Bahram Kasmapour Gang Pei Achim Gronow Maria V. Bianco Federico C. Blanco Christopher K. E. Bleck Robert Geffers Fabiana Bigi Wolf‐Rainer Abraham Maximiliano G. Gutierrez 《Cellular microbiology》2014,16(9):1425-1440
Some intracellular bacteria are known to cause long‐term infections that last decades without compromising the viability of the host. Although of critical importance, the adaptations that intracellular bacteria undergo during this long process of residence in a host cell environment remain obscure. Here, we report a novel experimental approach to study the adaptations of mycobacteria imposed by a long‐term intracellular lifestyle. Selected Mycobacterium bovis BCG through continuous culture in macrophages underwent an adaptation process leading to impaired phenolic glycolipids (PGL) synthesis, improved usage of glucose as a carbon source and accumulation of neutral lipids. These changes correlated with increased survival of mycobacteria in macrophages and mice during re‐infection and also with the specific expression of stress‐ and survival‐related genes. Our findings identify bacterial traits implicated in the establishment of long‐term cellular infections and represent a tool for understanding the physiological states and the environment that bacteria face living in fluctuating intracellular environments. 相似文献
59.
Age-stage,two-sex life table of the tomato looper,Chrysodeixis chalcites (Lepidoptera: Noctuidae), on different bean cultivars 总被引:1,自引:0,他引:1
Solmaz Alami Bahram Naseri Ali Golizadeh Jabraeil Razmjou 《Arthropod-Plant Interactions》2014,8(5):475-484
The tomato looper, Chrysodeixis chalcites (Esper), is one of the polyphagous pests of several economically crops worldwide. Two-sex life table parameters of C. chalcites reared on eight bean cultivars including white kidney bean (cultivars Daneshkadeh and Dehghan), red kidney bean (cultivars Goli and Naz), common bean (cultivars Khomein, Talash and Sadra) and cowpea (cultivar Mashhad) were studied under laboratory conditions (25 ± 1 °C, 65 ± 5 % RH, a 16:8-h light–dark photoperiod). The shortest larval period of C. chalcites was 14.15 days on common bean Sadra. The longest and shortest development time of total preadult was on white kidney bean Daneshkadeh and common bean Sadra (25.77 and 23.42 days, respectively). The highest total fecundity was on common bean Sadra (674.4 eggs), and the lowest was observed on white kidney bean Daneshkadeh (136.7 eggs). The intrinsic rate of increase (r m ) ranged from 0.0976 to 0.1599 female/female/day, which was lowest on white kidney bean Daneshkadeh and highest on common bean Sadra. The net reproductive rate (R 0) was highest on common bean Sadra (265.82 offspring) and lowest on white kidney bean Daneshkadeh (46.88 offspring). The results revealed that the cultivar Daneshkadeh was unsuitable host to C. chalcites in comparison to the other cultivars tested. 相似文献
60.
Mozhdeh Sharifipour Esmaeal Izadpanah Bahram Nikkhoo Samad Zare Ali Abdolmaleki Katayoun Hassanzadeh Farshid Moradi Kambiz Hassanzadeh 《Journal of biomedical science》2014,21(1):6