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排序方式: 共有360条查询结果,搜索用时 140 毫秒
101.
Tedersoo L Bahram M Jairus T Bechem E Chinoya S Mpumba R Leal M Randrianjohany E Razafimandimbison S Sadam A Naadel T Kõljalg U 《Molecular ecology》2011,20(14):3071-3080
Mycorrhizal fungi play a key role in mineral nutrition of terrestrial plants, but the factors affecting natural distribution, diversity and community composition of particularly tropical fungi remain poorly understood. This study addresses shifts in community structure and species frequency of ectomycorrhizal (EcM) fungi in relation to host taxa, soil depth and spatial structure in four contrasting African ecosystems. We used the rDNA and plastid trnL intron sequence analysis for identification of fungi and host plants, respectively. By partitioning out spatial autocorrelation in plant and fungal distribution, we suggest that African EcM fungal communities are little structured by soil horizon and host at the plant species and family levels. These findings contrast with patterns of vegetation in these forests and EcM fungal communities in other tropical and temperate ecosystems. The low level of host preference indirectly supports an earlier hypothesis that pioneer Phyllanthaceae may facilitate the establishment of late successional Fabaceae and potentially other EcM host trees by providing compatible fungal inoculum in deforested and naturally disturbed ecosystems of tropical Africa. 相似文献
102.
Sedaghati Behnam Haddad Raheem Bandehpour Mojgan Kazemi Bahram 《In vitro cellular & developmental biology. Plant》2022,58(6):921-930
In Vitro Cellular & Developmental Biology - Plant - Plants have long played a major role in human health as a source of herbal remedies. Recently, transgenic plants have become convenient... 相似文献
103.
Raphael Carapito Ekaterina L. Ivanova Aurore Morlon Linyan Meng Anne Molitor Eva Erdmann Bruno Kieffer Angélique Pichot Lydie Naegely Aline Kolmer Nicodème Paul Antoine Hanauer Frédéric Tran Mau-Them Nolwenn Jean-Marçais Susan M. Hiatt Gregory M. Cooper Tatiana Tvrdik Alison M. Muir Seiamak Bahram 《American journal of human genetics》2019,104(2):319-330
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Andrew Schoenrock Bahram Samanfar Sylvain Pitre Mohsen Hooshyar Ke Jin Charles A Phillips Hui Wang Sadhna Phanse Katayoun Omidi Yuan Gui Md Alamgir Alex Wong Fredrik Barren?s Mohan Babu Mikael Benson Michael A Langston James R Green Frank Dehne Ashkan Golshani 《BMC bioinformatics》2014,15(1)
Background
Our knowledge of global protein-protein interaction (PPI) networks in complex organisms such as humans is hindered by technical limitations of current methods.Results
On the basis of short co-occurring polypeptide regions, we developed a tool called MP-PIPE capable of predicting a global human PPI network within 3 months. With a recall of 23% at a precision of 82.1%, we predicted 172,132 putative PPIs. We demonstrate the usefulness of these predictions through a range of experiments.Conclusions
The speed and accuracy associated with MP-PIPE can make this a potential tool to study individual human PPI networks (from genomic sequences alone) for personalized medicine.Electronic supplementary material
The online version of this article (doi:10.1186/s12859-014-0383-1) contains supplementary material, which is available to authorized users. 相似文献106.
Marc Bronietzki Bahram Kasmapour Maximiliano Gabriel Gutierrez 《Journal of visualized experiments : JoVE》2014,(85)
Phagocytic cells play a major role in the innate immune system by removing and eliminating invading microorganisms in their phagosomes. Phagosome maturation is the complex and tightly regulated process during which a nascent phagosome undergoes drastic transformation through well-orchestrated interactions with various cellular organelles and compartments in the cytoplasm. This process, which is essential for the physiological function of phagocytic cells by endowing phagosomes with their lytic and bactericidal properties, culminates in fusion of phagosomes with lysosomes and biogenesis of phagolysosomes which is considered to be the last and critical stage of maturation for phagosomes. In this report, we describe a live cell imaging based method for qualitative and quantitative analysis of the dynamic process of lysosome to phagosome content delivery, which is a hallmark of phagolysosome biogenesis. This approach uses IgG-coated microbeads as a model for phagocytosis and fluorophore-conjugated dextran molecules as a luminal lysosomal cargo probe, in order to follow the dynamic delivery of lysosmal content to the phagosomes in real time in live macrophages using time-lapse imaging and confocal laser scanning microscopy. Here we describe in detail the background, the preparation steps and the step-by-step experimental setup to enable easy and precise deployment of this method in other labs. Our described method is simple, robust, and most importantly, can be easily adapted to study phagosomal interactions and maturation in different systems and under various experimental settings such as use of various phagocytic cells types, loss-of-function experiments, different probes, and phagocytic particles. 相似文献
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109.
Kheradmand A Dezfoulian O Alirezaei M Rasoulian B 《Biochemical and biophysical research communications》2012,419(2):299-304
Under normal condition in the most mammals, spermatogenesis is closely associated with the balance between germ cells proliferation and apoptosis. The present study was designed to determine the effects of ghrelin treatment on in vivo quality and quantity expression of apoptosis and proliferation specific indices in rat testicular germ cells. Twenty eight adult normal rats were subdivided into equal control and treatment groups. Treatment group received 3 nmol of ghrelin as subcutaneous injection for 30 consecutive days or vehicle to the control animals. The rats from each group (n=7) were killed on days 10 and 30 and their testes were taken for immunocytochemical evaluation and caspase-3 assay. Immunohistochemical analysis indicated that the accumulations of Bax and PCNA peptides are generally more prominent in spermatocytes and spermatogonia of both groups. Likewise, the mean percentage of immunoreactive spermatocytes against Bax increased (P<0.01) in the ghrelin-treated group on day 10, while despite of 30% increment in the Bax level of spermatocytes in the treated rats on day 30, however, it was not statistically significant. During the experimental period, only a few spermatogonia represented Bax expression and the changes of Bax immunolabling cells were negligible upon ghrelin treatment. Likewise, there were immunostaining cells against Bcl-2 in each germ cell neither in the control nor in the treated animals. In fact, ghrelin balanced Bax/Bcl-2 ratio toward at increase of Bax level in the spermatocytes and therefore may stimulate apoptosis in these germ cells. In contrast, ghrelin administration significantly suppressed proliferation-associated peptide PCNA in the spermatocytes as well as spermatogonia (P<0.05). Whereas, caspase-3 activity did not show any marked alteration during the experiment in both groups (P>0.05). Upstream of Bax substance parallel to down-regulation of PCNA demonstrate that ghrelin may prevent massive accumulation of germ cells during normal spermatogenesis. These observations also indicate that ghrelin may be considered as a modulator of spermatogenesis in normal adult rats and could be potentially implicated for abnormal spermatogenesis in some testicular germ cell tumors. 相似文献
110.
Julià L Amengual Antoni Valero-Cabré Misericordia Veciana de las Heras Nurja Rojo Seán Froudist-Walsh Pablo Ripollés Nils Bodammer Bahram Mohammadi Jordi Montero Carles Grau Thomas F Münte Antoni Rodríguez-Fornells 《BMC neurology》2012,12(1):1-14