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A method for the counting of mitochondria 总被引:2,自引:0,他引:2
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G F Bahr J C Lee 《Analytical and quantitative cytology and histology / the International Academy of Cytology [and] American Society of Cytology》1986,8(3):224-226
Chinese hamster metaphases were stained with dansyl chloride to determine protein. Using a microfluorometer, the fluorescence of chromosome number one and the total of the fluorescence of the metaphase were measured. The two measurements were found to be correlated with a coefficient of 0.640, suggesting that protein content in a single chromosome is a dependent variable of the protein content of the respective metaphase. 相似文献
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Variability of dry mass as a fundamental biological property demonstrated for the case of Vaccinia virions. 下载免费PDF全文
The dry mass of individual vaccinia virions, as an example of a presumably uniform biological population, prepared in different lost at the Institute for Tropical Hygiene in Hamburg, was tested for variability by quantitative electron microscopy. A value of 5.26 X 10(-15) g for the median weight of the particle was calculated from 7,300 determinations. By assessing the variability of polystyrene latex spheres, which were used as mass standards, we demonstrated that the variability of dry mass of vaccinia virions is fivefold greater than the variability (standard) introduced by the method for determining mass. It was concluded that while genetic control in a presumably homozygous virion is strict with respect to quality, quantity of viral components (other than DNA) varies in fashion that can be aptly described by a log-normal distribution. It is recognized that this observation is empirically supported by the paradigm that any composite biological entity is subject to quantitative variability, the more so the heavier the individual representatives of a species are. In addition, the effects that extractions and staining have on the dry mass of vaccinia are reported, as well as is the median for the dry mass of other strains of pox viruses. 相似文献
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