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Four hundred and ninety-eight predominantly pyocin-type 10 clinical strains of Pseudomonas aeruginosa were analyzed for resistance to carbenicillin, cefoperazone, cefotaxime, ceftazidime, gentamicin, amikacin and netilmicin. Based on NCCLS-recommended MIC breakpoints, 245 strains were found to be resistant, of which 41.6% were resistant to carbenicillin, 38% to gentamicin, 37.8% to netilmicin, 26.3% to cefoperazone, 17.9% to cefotaxime, 0.6% to amikacin and none to ceftazidime. Quadruple resistance to carbenicillin, cefoperazone, gentamicin and netilmicin was the most frequent pattern observed. Resistance to older antibiotics (kanamycin, streptomycin and tetracycline) and to mercuric chloride were also common. Conjugation experiments suggested that self-transmissible and non-transmissible plasmids occurred in at least 66 strains. 相似文献
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Noor Munirah Isa Azizan Baharuddin Saadan Man Lee Wei Chang 《Developing world bioethics》2015,15(3):143-151
The field of bioethics aims to ensure that modern scientific and technological advancements have been primarily developed for the benefits of humankind. This field is deeply rooted in the traditions of Western moral philosophy and socio‐political theory. With respect to the view that the practice of bioethics in certain community should incorporate religious and cultural elements, this paper attempts to expound bioethical tradition of the Malay‐Muslim community in Malaysia, with shedding light on the mechanism used by the National Fatwa Council to evaluate whether an application of biological sciences is ethical or not. By using the application of the genetically modified food as a case study, this study has found that the council had reviewed the basic guidelines in the main references of shari'ah in order to make decision on the permissibility of the application. The fatwa is made after having consultation with the experts in science field. The council has taken all factors into consideration and given priority to the general aim of shari'ah which to serve the interests of mankind and to save them from harm. 相似文献
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Tengku Haziyamin Tengku Abdul Hamid Raja Noor Zaliha Raja Abd Rahman Abu Bakar Salleh Mahiran Basri 《The protein journal》2010,29(4):290-297
The use of lipase in hydrophilic solvent is usually hampered by inactivation. The solvent stability of a recombinant solvent
stable lipase isolated from thermostable Bacillus sp. strain 42 (Lip 42), in DMSO and methanol were studied at different solvent-water compositions. The enzymatic activities
were retained in up to 45% v/v solvent compositions. The near-UV CD spectra indicated that tertiary structures were perturbed
at 60% v/v and above. Far-UV CD in methanol indicated the secondary structure in Lip 42 was retained throughout all solvent
compositions. Fluorescence studies indicated formations of molten globules in solvent compositions of 60% v/v and above. The
enzyme was able to retain its secondary structures in the presence of methanol; however, there was a general reduction in
β-sheet and an increase in α-helix contents. The H-bonding arrangements triggered in methanol and DMSO, respectively, caused
different forms of tertiary structure perturbations on Lip 42, despite both showing partial denaturation with molten globule
formations. 相似文献