全文获取类型
收费全文 | 703篇 |
免费 | 107篇 |
专业分类
810篇 |
出版年
2021年 | 10篇 |
2020年 | 6篇 |
2018年 | 6篇 |
2016年 | 15篇 |
2015年 | 26篇 |
2014年 | 22篇 |
2013年 | 25篇 |
2012年 | 32篇 |
2011年 | 33篇 |
2010年 | 27篇 |
2009年 | 19篇 |
2008年 | 30篇 |
2007年 | 33篇 |
2006年 | 18篇 |
2005年 | 23篇 |
2004年 | 17篇 |
2003年 | 24篇 |
2002年 | 20篇 |
2001年 | 20篇 |
2000年 | 21篇 |
1999年 | 8篇 |
1998年 | 11篇 |
1997年 | 21篇 |
1996年 | 8篇 |
1995年 | 9篇 |
1994年 | 12篇 |
1993年 | 8篇 |
1992年 | 14篇 |
1991年 | 22篇 |
1990年 | 13篇 |
1989年 | 20篇 |
1988年 | 12篇 |
1987年 | 11篇 |
1986年 | 12篇 |
1985年 | 16篇 |
1982年 | 11篇 |
1981年 | 7篇 |
1980年 | 11篇 |
1979年 | 7篇 |
1977年 | 12篇 |
1976年 | 6篇 |
1975年 | 8篇 |
1974年 | 8篇 |
1973年 | 6篇 |
1972年 | 11篇 |
1971年 | 11篇 |
1970年 | 8篇 |
1969年 | 8篇 |
1968年 | 10篇 |
1967年 | 9篇 |
排序方式: 共有810条查询结果,搜索用时 0 毫秒
11.
I Silberberg R L Baer S A Rosenthal G J Thorbecke V Berezowsky 《Cellular immunology》1975,18(2):435-453
In order to further define the role of Langerhans cells in contact allergic reactions, passive transfer studies were done in guinea pigs using 2,4-dinitro-1-chlorobenzene (DNCB)-sensitive donor cells. Langerhans cells were found in the lumen of dermal vessels resembling lymphatics at 2, 3, 15, and 48 hr after DNCB challenge. In contrast to the previously reported findings in actively sensitized guinea pigs, the changes involving Langerhans cells in passively sensitized guinea pigs were mainly noted in the dermis. These consisted of increased numbers of Langerhans cells and of mononuclear cells apposed to Langerhans cells 3 or more hours after challenge with DNCB. The increased Langerhans cell population in the dermis and the presence of Langerhans cells in dermal vessels in specifically challenged sites in adoptive immune reactions furnishes further support for a significant role of Langerhans cells in the interaction between antigen and sensitized cells. 相似文献
12.
Robert M. Baer 《Bulletin of mathematical biology》1975,37(1):59-69
An idealization of chemical combination is formulated as a model of computability, and it is shown that this model has universal computational power just in case assembly has at least two-dimensional space in which to occur. It is also shown that this model, under reinterpretation, corresponds to a cellular automaton in which growth occurs by differentiation only (i.e., the state into which any cell is born is thereadfter fixed). Hence this latter model of growth is also computationally universal. 相似文献
13.
The amount of insurance fraud is increasing in Canada. This should worry physicians, because all personal-injury claims must be substantiated by a medical certificate. The vast majority of physicians are honest and ethical, fraud investigators say, but some are being duped as patients scheme to cheat the insurance industry. In one sensational auto-insurance-fraud case, some Ontario physicians are being investigated about possible involvement in a self-referral scheme. Nicole Baer looks at insurance fraud and the challenges it poses for doctors. 相似文献
14.
Carlos García-Robledo Erin K. Kuprewicz Christina S. Baer Elizabeth Clifton Georgia G. Hernández David L. Wagner 《Biotropica》2020,52(4):590-597
Almost 40 years ago, Terry L. Erwin published a seemingly audacious proposition: There may be as many as 30 million species of insects in the world. Here, we translate Erwin's verbal argument into a diversity-ratio model—the Erwin Equation of Biodiversity—and discuss how it has inspired other biodiversity estimates. We categorize, describe the assumptions for, and summarize the most commonly used methods for calculating estimates of global biodiversity. Subsequent diversity-ratio extrapolations have incorporated parameters representing empirical insect specialization ratios, and how insect specialization changes at different spatial scales. Other approaches include macroecological diversity models and diversity curves. For many insect groups with poorly known taxonomies, diversity estimates are based on the opinions of taxonomic experts. We illustrate our current understanding of insect diversity by focusing on the six most speciose insect orders worldwide. For each order, we compiled estimates of the (a) maximum estimated number of species, (b) minimum estimated number of species, and (c) number of currently described species. By integrating these approaches and considering new information, we believe an estimate of 5.5 million species of insects in the world is much too low. New molecular methodologies (e.g., metabarcoding and NGS studies) are revealing daunting numbers of cryptic and previously undescribed species, at the same time increasing our precision but also uncertainty about present estimates. Not until technologies advance and sampling become more comprehensive, especially of tropical biotas, will we be able to make robust estimates of the total number of insect species on Earth. 相似文献
15.
Austin Burt Jacob G. Bundy Charles F. Baer 《Evolution; international journal of organic evolution》2016,70(10):2239-2246
A properly functioning organism must maintain metabolic homeostasis. Deleterious mutations degrade organismal function, presumably at least in part via effects on metabolic function. Here we present an initial investigation into the mutational structure of the Caenorhabditis elegans metabolome by means of a mutation accumulation experiment. We find that pool sizes of 29 metabolites vary greatly in their vulnerability to mutation, both in terms of the rate of accumulation of genetic variance (the mutational variance, VM) and the rate of change of the trait mean (the mutational bias, ΔM). Strikingly, some metabolites are much more vulnerable to mutation than any other trait previously studied in the same way. Although we cannot statistically assess the strength of mutational correlations between individual metabolites, principal component analysis provides strong evidence that some metabolite pools are genetically correlated, but also that there is substantial scope for independent evolution of different groups of metabolites. Averaged over mutation accumulation lines, PC3 is positively correlated with relative fitness, but a model in which metabolites are uncorrelated with fitness is nearly as good by Akaike's Information Criterion. 相似文献
16.
Anna G. Himler Eric J. Caldera Boris C. Baer Hermógenes Fernández-Marín Ulrich G. Mueller 《Proceedings. Biological sciences / The Royal Society》2009,276(1667):2611-2616
Asexual reproduction imposes evolutionary handicaps on asexual species, rendering them prone to extinction, because asexual reproduction generates novel genotypes and purges deleterious mutations at lower rates than sexual reproduction. Here, we report the first case of complete asexuality in ants, the fungus-growing ant Mycocepurus smithii, where queens reproduce asexually but workers are sterile, which is doubly enigmatic because the clonal colonies of M. smithii also depend on clonal fungi for food. Degenerate female mating anatomy, extensive field and laboratory surveys, and DNA fingerprinting implicate complete asexuality in this widespread ant species. Maternally inherited bacteria (e.g. Wolbachia, Cardinium) and the fungal cultivars can be ruled out as agents inducing asexuality. M. smithii societies of clonal females provide a unique system to test theories of parent–offspring conflict and reproductive policing in social insects. Asexuality of both ant farmer and fungal crop challenges traditional views proposing that sexual farmer ants outpace coevolving sexual crop pathogens, and thus compensate for vulnerabilities of their asexual crops. Either the double asexuality of both farmer and crop may permit the host to fully exploit advantages of asexuality for unknown reasons or frequent switching between crops (symbiont reassociation) generates novel ant–fungus combinations, which may compensate for any evolutionary handicaps of asexuality in M. smithii. 相似文献
17.
Carlos A. Mlaga Richard E. Weller Raymond L. Buschbom Janet F. Baer Barbara B. Kimsey 《Journal of medical primatology》1997,26(3):147-152
Abstract: The reproduction performance of captive owl monkeys, a breed used extensively in biomedical research, was observed at the Battelle Primate Facility (BPF). The colony grew through captive breeding, imports from the Peruvian Primatological Project, and others to a peak size of 730. It included seven karyotypes of Aotus sp. Results showed that owl monkeys can breed successfully in a laboratory in numbers sufficient to sustain modest research programs. Reproductive success increases when pairs are compatible, of the same karyotype, and stabilized; however, mated pairs of different karyotype are also productive. Under conditions of controlled lighting and heating, owl monkeys at BPF showed no birth peak nor birth season. 相似文献
18.
The tal gene undergoes chromosome translocation in T cell leukemia and potentially encodes a helix-loop-helix protein. 总被引:44,自引:13,他引:44 下载免费PDF全文
Q Chen J T Cheng L H Tasi N Schneider G Buchanan A Carroll W Crist B Ozanne M J Siciliano R Baer 《The EMBO journal》1990,9(2):415-424
We have analyzed t(1;14)(p32;q11) chromosome translocations from two patients with T cell acute lymphocytic leukemia. The chromosome 1 breakpoints of these patients lie within a kilobasepair of each other, and thus define a genetic locus (designated tal) involved in T cell oncogenesis. Moreover, we have identified sequences within tal that potentially encode an amphipathic helix-loop-helix motif, a DNA-binding domain found in a variety of proteins that control cell growth and differentiation. The homology domain of tal is especially related to that of lyl-1, a gene on chromosome 19 that has also been implicated in T cell oncogenesis. Hence, tal and lyl-1 encode a distinct family of helix-loop-helix proteins involved in the malignant development of lymphocytes. 相似文献
19.
Postcopulatory sexual selection, either in the form of sperm competition or cryptic female choice, is an important selective force that is thought to have generated the enormous variation in sperm morphology observed interspecifically. However, the evolutionary significance of intraspecific variation in sperm morphology, and the role that postcopulatory sexual selection plays in influencing this variation, remains poorly investigated in invertebrates. Here, we tested the hypothesis that postcopulatory sexual selection reduces variation in sperm morphology, both between and within males, in 27 species of eusocial ants and bees. These eusocial species offer an unusual opportunity to assess how selection acts on variance in sperm morphology, as haploid males produce clonal, haploid sperm that does not experience haploid-diploid conflict. We provide solid evidence that males of polyandrous ant and bee species indeed produce less-variable sperm, indicating that sperm competition selected for sperm of superior quality. Our results offer a mechanistic explanation for the evolution of high-quality sperm and provide comprehensive evidence that sperm morphology of social insects is influenced by sexual selection. 相似文献
20.
BRCA1 functions independently of homologous recombination in DNA interstrand crosslink repair 总被引:1,自引:0,他引:1
Bunting SF Callén E Kozak ML Kim JM Wong N López-Contreras AJ Ludwig T Baer R Faryabi RB Malhowski A Chen HT Fernandez-Capetillo O D'Andrea A Nussenzweig A 《Molecular cell》2012,46(2):125-135
Brca1 is required for DNA repair by homologous recombination (HR) and normal embryonic development. Here we report that deletion of the DNA damage response factor 53BP1 overcomes embryonic lethality in Brca1-nullizygous mice and rescues HR deficiency, as measured by hypersensitivity to polyADP-ribose polymerase (PARP) inhibition. However, Brca1,53BP1 double-deficient cells are hypersensitive to DNA interstrand crosslinks (ICLs), indicating that BRCA1 has an additional role in DNA crosslink repair that is distinct from HR. Disruption of the nonhomologous end-joining (NHEJ) factor, Ku, promotes DNA repair in Brca1-deficient cells; however deletion of either Ku or 53BP1 exacerbates genomic instability in cells lacking FANCD2, a mediator of the Fanconi anemia pathway for ICL repair. BRCA1 therefore has two separate roles in ICL repair that can be modulated by manipulating NHEJ, whereas FANCD2 provides a key activity that cannot be bypassed by ablation of 53BP1 or Ku. 相似文献