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31.
Karamouzis MV Badra FA Papavassiliou AG 《The international journal of biochemistry & cell biology》2007,39(5):851-856
Breast cancer is the most common cancer in women and the ErbB receptor family holds crucial role in its pathogenesis. Among them, epidermal growth factor receptor and HER-2 are the most studied members and their overexpression has been associated with aggressive clinical behaviour. These data were further strengthened by the clinical success of trastuzumab, a monoclonal antibody against HER-2 in breast cancer patients with HER-2 overexpression and/or amplification. However, trastuzumab failure in some patients may partly be attributed to co-expression of other ErbB receptors. Herein, we provide updated views regarding the role of HER-3 and HER-4 in breast cancer. Accumulated evidence implies that these receptors should be considered more than heterodimerisation partners. Their expression profile might be useful in predicting responsiveness to current treatment options, while new strategies targeting their ligands and downstream effectors are being developed. 相似文献
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Inferences for a semiparametric model with panel data 总被引:1,自引:0,他引:1
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A.A.leslie Gunatilaka Sarath R. Sirimanne Subramaniam Sotheeswaran H.T.Badra Sriyani 《Phytochemistry》1982,21(3):805-806
From the bud exudates of Gardenia cramerii and G. fosbergii, two species endemic to Sri Lanka, a new flavonoid with an unusual B-ring oxidation pattern, 5,5′-dihydroxy-6,7,2′,3′-tetramethoxyflavone, was characterized. Two other rare flavonoids, 5,3′,5′-trihydroxy-3,6,7,4′-tetramethoxyflavone and 5-hydroxy-6,7,3′,4′,5′-pentamethoxyflavone were also isolated from both Gardenia species. 相似文献
34.
Badra Bouamama Asma Ben Salem Fatma Ben Youssef Soumaya Chaieb Mohamed-Hbib Jaafoura Ahmed Mliki Abdelwahed Ghorbel 《In vitro cellular & developmental biology. Plant》2011,47(2):321-327
The regeneration ability of a Tunisian barley accession originated from Kerkena islands was monitored through somatic embryogenesis
and organogenesis. To prevent or to reduce normal germination, longitudinally bisected as well as base-wounded mature caryopses
were cultured on a modified Chée and Pool-based medium (CP) enriched with different phytohormonal combinations. The greatest
embryogenesis response was obtained when base-wounded caryopses were cultured on CP enriched with 2 mg/l chlorophenoxyacetic
acid + 2.5 mg/l kinetin (76.85%). The same combination coupled to longitudinally bisected caryopses led to the embryogenic
induction at the hypocotyl base of the germinated caryopses (61.9%). Embryogenic calluses differentiated into globular, heart-shaped,
torpedo, and fully differentiated stages of somatic embryos on hormone-free Murashige and Skoog-based medium. Rooted plantlets
were successfully transferred to soil and grown to maturity in the greenhouse and produced fertile seeds within 3 mo. On the
other hand, organogenesis was achieved on CP enriched with 2 mg/l 2,4-dichlorophenoxyacetic acid + 2.5 mg/l kinetin. Histological
aspects and scanning electron microscopy of both regeneration methods confirmed further the embryogenic and organogenic nature
of the established processes. This efficient plant regeneration system provides a foundation for generating transgenic plants
and germplasm preservation of “Kerkena” barley accession. 相似文献
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A.A.Leslie Gunatilaka H.T.Badra Sriyani Subramaniam Sotheeswaran 《Phytochemistry》1984,23(11):2679-2681
The petrol extractives of the bark of Garcinia quaesita gave hermonionic acid, its decarboxylated product and a new phenol, quaesitol. 相似文献
38.
为建立鸭乙型肝炎病毒LJ-76的转染细胞系,将LJ-76病毒DNA插入到pUC19的EcoRⅠ位点上,分离得到含有双拷贝LJ-76DNA的重组质粒.通过磷酸钙沉淀方法,将经CsCl等密度离心纯化的LJ-76DNA双体导入到人肝癌细胞BEL7402中.收集转染细胞的培养液进行蔗糖密度梯度离心,所得沉淀经检测发现含有LJ-76DNA并具有特异性DHBV内源性DNA多聚酶活性;对上述样品通过DotEIA检测DHBV核心抗原及表面抗原结果为阳性.Southernblot分析表明转染细胞内存在病毒DNA复制中间体cccDNA、ssDNA和rcDNA,而cccDNA被认为是复制活动较为活跃的标志.电镜观察转染细胞的上清发现有病毒颗粒的存在. 相似文献
39.
IV Zlatkin M Schneider FJ de Bruijn LJ Forney PhD 《Journal of industrial microbiology & biotechnology》1996,17(3-4):219-227
Culturable bacteria from the deep subsurface (179 m) at Cerro Negro, New Mexico were isolated and characterized. The average number of viable aerobic bacteria was estimated to be 5×105g–1 of sediment, but only about 0.1% of these could be recovered on agar medium when incubated under aerobic conditions. Of 158 strains isolated from this depth, 92 were characterized by cellular fatty acid profiles (FAME), 36 by analysis of partial 16S rDNA sequences, and 44 by rep-PCR genome fingerprint analysis using three different sets of oligonucleotide primers (REP, BOX, or ERIC). These analyses showed the majority of isolates (67%) were Gram-positive bacteria and primarily members of genera with a high %G+C DNA. The remaining isolates were -subdivisionProteobacteria (19%) and members of the flavobacteria group (14%). The diversity indices based on these different methods of characterization were very high suggesting this subsurface habitat harbors a highly diverse microbial community. 相似文献
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Manel Borji Badra Bouamama-Gzara Farhat Chibani Caroline Teyssier Awatef Ben Ammar Ahmed Mliki Sami Zekri Abdelwahed Ghorbel 《Plant Cell, Tissue and Organ Culture》2018,132(2):329-342
To better understand micromorphological and structural changes, histological sections provide additional insight into cellular process and developmental pathways occurring in oat somatic embryogenesis. Environmental scanning electron microscopy (ESEM) and transmission electron microscopy (TEM) were also used to follow the ultrastructural modifications during this system. Histological observations allowed following the events leading to the development of mature somatic embryos. The scheme includes the following steps: cell reactivation, the first organized cell division in diads, triads, tetrads as well as octant stages, the observation of an extracellular matrix (ECM) as a fibrillar material that bounded the surface of individualized proembryos. The transition from proembryo stage to an early globular somatic embryo was noted, where the embryogenic cortex is surrounded by the protoderm. The late globular stage was marked by bipolarity. The early and late transitional stages, the coleoptilar, mature and germinated stages were also described. The ESEM allowed us to follow some rearrangements, related to the morphology and surfaces involved in somatic embryos development. These events are proembryo formation, transition from proembryo to globular stage, marked by protoderm formation, scutellum and coleoptile development and finally somatic embryos germination. The TEM showed that embryogenic cells were very rich in organelles; mitochondria, rough endoplasmic reticulum, Golgi apparatus and ribosomes. Cells of proembryos, globular and late somatic embryos showed more vacuoles and differentiated organelles. The ECM was also detected by TEM as fibrillar material coating the cell walls. These results on structural and ultrastructural changes provided new insights and findings on oat somatic embryogenesis. 相似文献