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排序方式: 共有214条查询结果,搜索用时 15 毫秒
31.
Background
Epidemiological studies have shown that the offspring of mothers who experience diabetes mellitus during pregnancy are seven times more likely to develop health complications later in life compared to offspring born to nondiabetic mothers.Aim of the study
We investigated whether supplementation with a natural antioxidant (thymoquinone; TQ) in female rats with streptozotocin (STZ)-induced gestational diabetes (GD) improved diabetic complications and T cell immune responses in their offspring.Methods
Three groups of female rats were tested: nondiabetics, diabetics treated with TQ during pregnancy and lactation periods and diabetics that were not treated with TQ (n = 10 female rats in each group).Results
Our data demonstrated a significant decrease in the numbers of neonates born to diabetic rats compared with those born to control rats. GD led to macrosomic pups with several postpartum complications, such as a significant increase in plasma levels of the pro-inflammatory cytokines IL-1β, IL-6, and TNF-α (but not of IL-10); a marked decrease in the plasma level of IL-2; a marked reduction in the proliferative capacity of superantigen (SEB)-stimulated T-lymphocytes; and an obvious reduction in the number of circulating and thymus homing T cells. TQ supplementation of diabetic mothers during pregnancy and lactation periods had an obvious and significant effect on the number and mean body weight of neonates. Furthermore, TQ significantly restored the IL-2 level and T cell proliferation and subsequently rescued both circulating and thymus homing T cells in the offspring.Conclusions
Our data suggest that nutritional supplementation of GD mothers with the natural antioxidant TQ during pregnancy and lactation periods improves diabetic complications and maintains an efficient T cell immune response in their offspring, providing a protective effect in later life. 相似文献32.
Tongue keratinocytes have a high mitotic index (MI) with an evident circadian variation. Our study set out to compare and contrast two phases of the cell cycle: DNA synthesis (S-phase), with inmunocytochemical detection by bromodeoxyuridine (BrdU), and mitosis (M-phase), by the colchicine-arrest of metaphase method, exploring both the dorsal and ventral surfaces of the mouse tongue throughout a circadian period. Adult male mice standardized for light periodicity used for MI experiment were injected intraperitoneally with colchicine. Other animals were injected intraperitoneally with 5-BrdU for S-phase determination. Animals given both treatments were divided into six groups and killed at 4 h intervals until 20:00 h. Tongue samples were processed for histology and immuno-histochemistry. S and M indices were expressed as labelled nuclei or colchicine metaphases, respectively, per 1000 nuclei. Peak MI occurred at 12:00, with the minimum value at 20:00 on dorsal and ventral tongue surfaces. Peak S-phase was at 04:00, whereas the minimum value was at 16:00 for both surfaces. These results show that the proliferative activity of the tongue epithelium is of similar intensity and temporal distribution on both surfaces. 相似文献
33.
Hala Badr Khalil Sabrina C Brunetti Uyen Minh Pham Deborah Maret André Laroche Patrick J Gulick 《BMC genomics》2014,15(1)
Background
The caleosin genes encode proteins with a single conserved EF hand calcium-binding domain and comprise small gene families found in a wide range of plant species. Some members of the gene family have been shown to be upregulated by environmental stresses including low water availability and high salinity. Caleosin 3 from wheat has been shown to interact with the α-subunit of the heterotrimeric G proteins, and to act as a GTPase activating protein (GAP). This study characterizes the size and diversity of the gene family in wheat and related species and characterizes the differential tissue-specific expression of members of the gene family.Results
A total of 34 gene family members that belong to eleven paralogous groups of caleosins were identified in the hexaploid bread wheat, T. aestivum. Each group was represented by three homeologous copies of the gene located on corresponding homeologous chromosomes, except the caleosin 10, which has four gene copies. Ten gene family members were identified in diploid barley, Hordeum vulgare, and in rye, Secale cereale, seven in Brachypodium distachyon, and six in rice, Oryza sativa. The analysis of gene expression was assayed in triticale and rye by RNA-Seq analysis of 454 sequence sets and members of the gene family were found to have diverse patterns of gene expression in the different tissues that were sampled in rye and in triticale, the hybrid hexaploid species derived from wheat and rye. Expression of the gene family in wheat and barley was also previously determined by microarray analysis, and changes in expression during development and in response to environmental stresses are presented.Conclusions
The caleosin gene family had a greater degree of expansion in the Triticeae than in the other monocot species, Brachypodium and rice. The prior implication of one member of the gene family in the stress response and heterotrimeric G protein signaling, points to the potential importance of the caleosin gene family. The complexity of the family and differential expression in various tissues and under conditions of abiotic stress suggests the possibility that caleosin family members may play diverse roles in signaling and development that warrants further investigation.Electronic supplementary material
The online version of this article (doi:10.1186/1471-2164-15-239) contains supplementary material, which is available to authorized users. 相似文献34.
X S Zhou S Shahabuddin B R Zahn M A Babcock M S Badr 《Journal of applied physiology》2000,89(1):192-199
We hypothesized that a decreased susceptibility to the development of hypocapnic central apnea during non-rapid eye movement (NREM) sleep in women compared with men could be an explanation for the gender difference in the sleep apnea/hypopnea syndrome. We studied eight men (age 25-35 yr) and eight women in the midluteal phase of the menstrual cycle (age 21-43 yr); we repeated studies in six women during the midfollicular phase. Hypocapnia was induced via nasal mechanical ventilation for 3 min, with respiratory frequency matched to eupneic frequency. Tidal volume (VT) was increased between 110 and 200% of eupneic control. Cessation of mechanical ventilation resulted in hypocapnic central apnea or hypopnea, depending on the magnitude of hypocapnia. Nadir minute ventilation in the recovery period was plotted against the change in end-tidal PCO(2) (PET(CO(2))) per trial; minute ventilation was given a value of 0 during central apnea. The apneic threshold was defined as the x-intercept of the linear regression line. In women, induction of a central apnea required an increase in VT to 155 +/- 29% (mean +/- SD) and a reduction of PET(CO(2)) by -4.72 +/- 0.57 Torr. In men, induction of a central apnea required an increase in VT to 142 +/- 13% and a reduction of PET(CO(2)) by -3.54 +/- 0.31 Torr (P = 0.002). There was no difference in the apneic threshold between the follicular and the luteal phase in women. Premenopausal women are less susceptible to hypocapnic disfacilitation during NREM sleep than men. This effect was not explained by progesterone. Preservation of ventilatory motor output during hypocapnia may explain the gender difference in sleep apnea. 相似文献
35.
36.
Abdelfattah Badr Hoida Zaki Mousa O. Germoush Abdelrahim Q. Tawfeek Mohamed A. El-Tayeb 《Acta Physiologiae Plantarum》2013,35(6):1933-1941
The hazardous potential of the Metosulam herbicide, particularly the cytogenetic and physiological effects on Vicia faba cv Assuit 25 plants has been studied. The results showed that the mitotic index (MI) decreased and chromosomal aberrations frequency increased by increasing of the concentration of herbicide and prolonging the duration of treatment. In the roots treated with highest concentration used (1 × 10?5 %) for 24 h, complete inhibition of cell division was observed. The chromosomal anomalies include chromosomal bridges and breaks that are regarded were indicative of a mutagenic potential of the herbicide. Seedling growth (fresh and dry weight) adversely affected as the duration and concentration of Metosulam herbicide increased. Soluble sugars, soluble proteins, total free amino acids and photosynthetic pigment content decreased significantly in root, stem and leaves of Vicia faba with increasing both the herbicide concentration and treatment duration. In contrast, proline content was highly accumulated, especially at the highest concentration (10?4 %) and the longest duration used (24 h). The results of antioxidant enzymes reveal that while the peroxidase activity decreased by increasing the concentration of herbicide and duration, the activities of catalase and ascorbate peroxidase increased. 相似文献
37.
Badr Hadhidh Alharbi Mohammad Jahangir Pasha Abdulrahman Hussein Alhudhodi Abdulilah Khalid Alduwais 《Soil & Sediment Contamination》2018,27(8):674-691
ABSTRACTSpills and leakage from underground fuel storage tanks (UFSTs) can potentially contaminate soil and groundwater and pose harmful effects to public health and the environment. This study evaluated the feasibility of using volatile organic compounds (VOCs), total petroleum hydrocarbons (TPHs), electrical conductivity (EC), and pH to examine the contamination caused by leaking UFSTs. Screening water assessments for VOCs and general water quality parameters were conducted on the premises of 53 gas stations in Riyadh, Saudi Arabia, to identify potentially contaminated sites, and 25 ground bores were drilled for the quantification of TPH concentrations, EC, and pH values in 407 soil samples. The experimental approach followed in this study included geochemical analyses based on borehole drilling at five targeted gas stations, analyses of water samples from underground storage reservoirs, and analyses of soil core samples obtained from different depths to determine the degree of TPH contamination. Thirty-five VOCs were identified in the water samples collected from gas stations. Methylene chloride, tribromomethane, toluene, chlorobenzene, dibromochloromethane, and benzene were frequently encountered in most of the water samples. Some of these samples exceeded the World Health Organization and Saudi Arabian guidelines for acceptable levels of pH, total dissolved solids, chloride, nitrate, sulfate, calcium, and total hardness. The measured TPH levels were clearly indicative of subsoil contamination and subsequent accumulation in soil over time, particularly at depths of 1–6 m; there was not a noticeable dependence or impact on pH. 相似文献
38.
Kathrin Krause Kyle Caution Asmaa Badr Kaitlin Hamilton Abdulmuti Saleh Khushbu Patel 《Autophagy》2018,14(11):1928-1942
CASP4/caspase-11-dependent inflammasome activation is important for the clearance of various Gram-negative bacteria entering the host cytosol. Additionally, CASP4 modulates the actin cytoskeleton to promote the maturation of phagosomes harboring intracellular pathogens such as Legionella pneumophila but not those enclosing nonpathogenic bacteria. Nevertheless, this non-inflammatory role of CASP4 regarding the trafficking of vacuolar bacteria remains poorly understood. Macroautophagy/autophagy, a catabolic process within eukaryotic cells, is also implicated in the elimination of intracellular pathogens such as Burkholderia cenocepacia. Here we show that CASP4-deficient macrophages exhibit a defect in autophagosome formation in response to B. cenocepacia infection. The absence of CASP4 causes an accumulation of the small GTPase RAB7, reduced colocalization of B. cenocepacia with LC3 and acidic compartments accompanied by increased bacterial replication in vitro and in vivo. Together, our data reveal a novel role of CASP4 in regulating autophagy in response to B. cenocepacia infection. 相似文献
39.
Stéphane Pfendler Badr Alaoui-Sossé Laurence Alaoui-Sossé Faisl Bousta Lotfi Aleya 《Journal of applied phycology》2018,30(3):1607-1616
Photosynthetic biofilms proliferating on heritage monuments represent a major threat for curators leading to biodegradation and esthetic issues. Previous studies demonstrated that UV-C, used as a tool for biofilm eradication, is a promising avenue to combat microbial proliferation. In this study, this environmentally friendly method was tested on biofilm-forming Chlorella vulgaris suspension. Algal physiological response to UV-C was then assessed. Results showed that >?10 kJ m?2 UV-C exposure was enough to directly kill cells whereas low UV-C exposure reduced quantum yield of photosystem II and inhibited both respiration and photosynthesis. Clear relationships between UV-C exposure times and physiological responses were found. In addition, the use of VIS-light after UV-C treatment enhances chlorophyll bleaching. Our findings contribute to a better understanding of the physiological responses of Chlorella vulgaris to UV-C radiation allowing thus an optimization of the UV-C treatment reported in our previous studies. 相似文献
40.
It has been suggested that peroxisome proliferators stimulate Kupffer cells, an effect which may be involved in their mechanism of action. To evaluate this hypothesis, this study was designed to investigate the effect of stimulating Kupffer cells on basal as well as induced peroxisomal enzyme activity. Twenty four hours following treatment of male Sprague-Dawley rats with the peroxisome proliferating agent perfluorooctanoic acid (PFOA), in corn oil or with corn oil alone, hepatic peroxisomal -oxidation was 4.6 ± 0.2 and 1.8 ± 0.1 U/g liver, respectively. As expected, PFOA did not influence the catalase activity. Stimulating Kupffer cells in vivo by zymosan A (25 mg/kg, iv) prior to treatment with corn oil or PFOA diminished basal as well as PFOA-induced peroxisomal b-oxidation by 20-35%. Activation of Kupffer cells by zymosan A also diminished catalase activity by over 60%. Furthermore, PFOA reduced blood colloidal carbon clearance by 35% within 2 h of its administration. The data suggest that activation of Kupffer cells exerts a negative effect on basal as well as PFOA-induced peroxisomal enzyme activities. Data also suggest that PFOA inhibits Kupffer cells. Activated Kupffer cells may indeed produce factors which interfere with normal hepatic peroxisomal functions and responses. 相似文献