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Internal Representations of Temporal Statistics and Feedback Calibrate Motor-Sensory Interval Timing
Humans have been shown to adapt to the temporal statistics of timing tasks so as to optimize the accuracy of their responses, in agreement with the predictions of Bayesian integration. This suggests that they build an internal representation of both the experimentally imposed distribution of time intervals (the prior) and of the error (the loss function). The responses of a Bayesian ideal observer depend crucially on these internal representations, which have only been previously studied for simple distributions. To study the nature of these representations we asked subjects to reproduce time intervals drawn from underlying temporal distributions of varying complexity, from uniform to highly skewed or bimodal while also varying the error mapping that determined the performance feedback. Interval reproduction times were affected by both the distribution and feedback, in good agreement with a performance-optimizing Bayesian observer and actor model. Bayesian model comparison highlighted that subjects were integrating the provided feedback and represented the experimental distribution with a smoothed approximation. A nonparametric reconstruction of the subjective priors from the data shows that they are generally in agreement with the true distributions up to third-order moments, but with systematically heavier tails. In particular, higher-order statistical features (kurtosis, multimodality) seem much harder to acquire. Our findings suggest that humans have only minor constraints on learning lower-order statistical properties of unimodal (including peaked and skewed) distributions of time intervals under the guidance of corrective feedback, and that their behavior is well explained by Bayesian decision theory. 相似文献
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Sequence complexity and diversity of polyadenylated RNA molecules transcribed in human myeloid cells
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Alberto Buson Alessandro Negro Luigi Grassato Massimo Tagliaro Marina Basaglia Claudio Grandi Angelo Fontana Marco P. Nuti 《FEMS microbiology letters》1996,145(1):55-62
Abstract A clone positive for d-carbamoylase activity (2.7 kb Hin dIII- Bam H1 DNA fragment) was obtained by screening a genomic library of Agrobacterium radiobacter in Escherichia coli . This DNA fragment contains an open reading frame of 912 bp which is predicted to encode a peptide of 304 amino acids with a calculated molecular mass of 34247 Da. The d-carbamoylase gene. named cauA , was placed under the control of T7 RNA-dependent promoter and expressed in E. coli BL21 (DE3). After induction with isopropyl-thio-β-d-galactopyranoside, the synthesis of d-carbamoylase in E. coli reached about 40% of the total protein. The expressed protein was shown to possess a molecular mass, on SDS-PAGE, of 36 kDa and showed an enhanced allowed us to establish that a Pro14 →Leu14 exchange leads to an inactive enzyme species, while a Cys279 →Ser279 exchange did not impair the functional properties of the enxyme. 相似文献
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Maintenance of photosynthesis at low leaf water potential in wheat : role of potassium status and irrigation history 总被引:2,自引:2,他引:0 下载免费PDF全文
The interaction of low water potential effects on photosynthesis, and leaf K+ levels in wheat (Triticum aestivum L.) plants was studied. Plants were grown at three K+ fertilization levels; 0.2, 2, and 6 millimolar. With well watered plants, 2 millimolar K+ supported maximal photosynthetic rates; 0.2 millimolar K+ was inhibitory, and 6 millimolar K+ was superoptimal (i.e. rates were no greater than at 2 millimolar K+). Photosynthesis was monitored at high (930 parts per million) and low (330 parts per million) external CO2 throughout a series of water stress cycles. Plants subjected to one stress cycle were considered nonacclimated; plants subjected to two successive cycles were considered acclimated during the second cycle. Sensitivity of photosynthesis to declining leaf water potential was affected by K+ status; 6 millimolar K+ plants were less sensitive, and 0.2 millimolar K+ plants were more sensitive than 2 millimolar K+ plants to declining water potential. This occurred with nonacclimated and acclimated plants at both high and low assay CO2. It was concluded that the K+ effect on photosynthesis under stress was not mediated by treatment effects on stomatal resistance. Differences between the K+ treatments were much less pronounced, however, when photosynthesis of nonacclimated and acclimated plants was plotted at a function of declining relative water content during the stress cycles. These results suggest that K+ effects on the relationship between relative water content and water potential in stressed plants was primarily responsible for the bulk of the K+-protective effect on photosynthesis in stressed plants. In vitro experiments with chloroplasts and protoplasts isolated from 2 millimolar K+ and 6 millimolar K+ plants indicated that upon dehydration, K+ efflux from the chloroplast stroma into the cytoplasm is less pronounced in 6 millimolar K+ protoplasts. 相似文献