Transitional cell carcinoma matrix stiffness regulates the osteopontin and YAP expression in recurrent patients

Molecular Biology Reports - Cells translate the mechanosensing of extracellular matrix component dysregulation and stiffness into the signal transduction including Osteopontin (OPN) through the...     

Evaluation of the in vivo antihypertensive effect and antioxidant activity of HL-7 and HL-10 peptide in mice

Molecular Biology Reports - The tendency to use bioactive peptides has increased in recent decades, and research would be essential for recognizing the therapeutic effects of peptides present in...     

Electrospray Production of Curcumin-walnut Protein Nanoparticles

Electrospray technique was applied to produce nanoparticles (NPs) from walnut protein isolate (WPI) in order to encapsulate curcumin. The optimized condition for the production of the WPI NPs was WPI concentration of 3.5%, a solvent mixture of deionized water: 2-propanol (2:1, v/v), electrospraying at a fixed distance, voltage and flow rate including 17.5 cm, 20 kV and 0.09 mL/h, respectively. The ratio of curcumin to WPI was 1:100 (w/w), and the encapsulation efficiency was around 61.5%. The addition of curcumin to WPI solution affected the size, form and surface properties of the electrosprayed NPs. FESEM, HRTEM, AFM, XRD, FT-IR and fluorescence spectroscopy, were applied for the characterization of the produced NPs via electrospraying. The in vitro study revealed that release of curcumin was limited in the stomach due to the protective effect of WPI NPs. In the small intestine, WPI particles were hydrolyzed and curcumin was released. Furthermore, the antioxidant activity of curcumin-WPI NPs after in vitro digestion was significantly higher than WPI NPs due to the bioactive peptides released from WPI NPs. It can be concluded that WPI NPs could act as a unique food-grade carrier to boost the solubility of curcumin.

     

Experimental Analysis of E2BB (LTIIb) Signal Peptide in Secretory Production of Reteplase in Escherichia coli

International Journal of Peptide Research and Therapeutics - Recombinant reteplase is the truncated form of tissue plasminogen activator. Signal peptides play a pivotal role in the secretion of...     

Growth,Physiological and Biochemical Traits of Sage under the Exogenous Stimulating and Stress Factors

Russian Journal of Plant Physiology - Sage (Salvia officinalis L.) belonging to the Lamiaceae family, as the main herb in the world is cultivated in many countries for extraction of essential oil...     

In Silico Designing a Candidate Vaccine Against Breast Cancer

International Journal of Peptide Research and Therapeutics - Breast cancer (BC) is the most common type of women’s cancer with a prevalence of about 25%, although it is rare in men...     

Identification and Validation of a Putative Polycomb Responsive Element in the Human Genome

Epigenetic cellular memory mechanisms that involve polycomb and trithorax group of proteins are well conserved across metazoans. The cis-acting elements interacting with these proteins, however, are poorly understood in mammals. In a directed search we identified a potential polycomb responsive element with 25 repeats of YY1 binding motifthatwe designate PRE-PIK3C2B as it occurs in the first intron of human PIK3C2B gene. It down regulates reporter gene expression in HEK cells and the repression is dependent on polycomb group of proteins (PcG). We demonstrate that PRE-PIK3C2B interacts directly with YY1 in vitro and recruits PRC2 complex in vivo. The localization of PcG proteins including YY1 to PRE-PIK3C2B in HEK cells is decreased on knock-down of either YY1 or SUZ12. Endogenous PRE-PIK3C2B shows bivalent marking having H3K27me3 and H3K4me3 for repressed and active state respectively. In transgenic Drosophila, PRE-PIK3C2B down regulates mini-white expression, exhibits variegation and pairing sensitive silencing (PSS), which has not been previously demonstrated for mammalian PRE. Taken together, our results strongly suggest that PRE-PIK3C2B functions as a site of interaction for polycomb proteins.     

Phytotoxicity and antifungal properties of the essential oil from the Juniperus polycarpos var. turcomanica (B. Fedsch.) R.P. Adams leaves

This study was performed to investigate the constituents, in vitro antifungal activity and phytotoxicity potential of the essential oil from Juniperus polycarpos var. turcomanica leaves. The essential oil was analyzed by GC–FID, and GC/MS, which predominantly contains α-pinene (51.21%), germacrene–B (4.80%), and ∆-cadinene (2.56%). The antifungal activity of the essential oil against some phytopathogenic fungi, including Alternaria alternata, Colletotrichum trichellum, Curvularia fallax, Cytospora sacchari, Fusarium oxysporum, and Macrophomina phaseolina was performed through disk diffusion and agar dilution assays. The essential oil of J. polycarpos var. turcomanica had high antifungal activity against tested phytopathogenic fungi. The most susceptible fungi to the essential oil were C. trichellum in agar dilution and M. phaseolina and C. fallax in disk diffusion methods, whereas, the most resistant fungus to the essential oil was obtained from A. alternata in both assays. Screening methods had an influence on antifungal activity of the essential oil as most of the tested fungi in this study were shown to be more resistant in disc diffusion methods. According to the phytotoxic assay results, the essential oil from J. polycarpos var. turcomanica had high phytotoxicity against three species of weeds, including P. oleracea L., A. retroflexus L., and D. stramonium L. The results of this research suggest that the herbicidal and antifungal activities of the essential oil from J. polycarpos var. turcomanica can be attributed to its major group of constituents, monoterpenes hydrocarbons.     

Prevention of thermal aggregation of an allosteric protein by small molecules: some mechanistic insights

Detailed knowledge of conformation and dynamics of native, intermediate and unfolded states of a protein is essential in searching for effective small molecules to prevent its aggregation. In a recent study we have demonstrated how allosteric effectors may influence protein-protein interactions at high temperatures using glutamate dehydrogenase (GDH) as a model allosteric protein. In the present study, thermal aggregation of this well-characterized enzyme was investigated in the presence of a number of amino acids (including Gly, Glu, Trp, Pro, Lys, Arg), polyamines (putrescine and spermidine) and chaperone-like molecules (cyclodextrins and caseins) as non-specific effectors. It was shown that some amino acids and polyamines may suppress aggregation via interaction with native species and may preserve the activity of the enzyme while cyclodextrins and caseins may exert their anti-aggregation potential via binding to aggregation-prone intermediates, without having any capacity to protect its native structure from unfolding. Observations describing the similarities and differences between the specific ligands and non-specific small molecules related to their interaction with native and aggregation-prone states of GDH are presented and discussed. It is argued that the type of studies described in the present communication is useful for the development of effective strategies for prevention of aggregation by small molecules.     

Biological activity of some plant essential oils against <Emphasis Type="Italic">Varroa destructor</Emphasis> (Acari: Varroidae), an ectoparasitic mite of <Emphasis Type="Italic">Apis mellifera</Emphasis> (Hymenoptera: Apidae)

This experiment was conducted to evaluate acaricidal activity of the essential oils of Thymus kotschyanus, Ferula assa-foetida and Eucalyptus camaldulensis against Varroa destructor under laboratory conditions. Moreover, fumigant toxicity of these oils was tested on Apis mellifera. After preliminary dose-setting experiments, mites and honey bees were exposed to different concentrations of the oil, with 10 h exposure time. Essential oil of T. kotschyanus appeared the most potent fumigant for V. destructor (LC₅₀ = 1.07, 95% confidence limit (CL) = 0.87–1.26 μl/l air), followed by E. camaldulensis (LC₅₀ = 1.74, 95% CL = 0.96–2.50 μl/l air). The lowest acaricidal activity (LC₅₀ = 2.46, 95% CL = 2.10–2.86 μl/l air) was attributed to essential oil of F. assa-foetida. Surprisingly, among the three oils tested, essential oil of T. kotschyanus had the lowest insecticidal activity against A. mellifera (LC₅₀ = 5.08, 95% CL = 4.54–5.06 μl/l air). These findings proved that essential oil of T. kotschyanus has potential of practical value for use as alternative acaricide in the management of varroa in apiaries.