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41.
Status of complete proteome analysis by mass spectrometry: SILAC labeled yeast as a model system 总被引:5,自引:1,他引:4
Background
Mass spectrometry has become a powerful tool for the analysis of large numbers of proteins in complex samples, enabling much of proteomics. Due to various analytical challenges, so far no proteome has been sequenced completely. O'Shea, Weissman and co-workers have recently determined the copy number of yeast proteins, making this proteome an excellent model system to study factors affecting coverage. 相似文献42.
Background
Optical imaging is an attractive non-invasive way to evaluate the expression of a transferred DNA, mainly thanks to its lower cost and ease of realization. In this study optical imaging was evaluated for monitoring and quantification of the mouse knee joint and tibial cranial muscle electrotransfer of a luciferase encoding plasmid. Optical imaging was applied to study the kinetics of luciferase expression in both tissues. 相似文献43.
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Raphael B. Costa Gregório MF Camargo Iara DPS Diaz Natalia Irano Marina M. Dias Roberto Carvalheiro Arione A. Boligon Fernando Baldi Henrique N. Oliveira Humberto Tonhati Lucia G. Albuquerque 《遗传、选种与进化》2015,47(1)
Background
An important goal of Zebu breeding programs is to improve reproductive performance. A major problem faced with the genetic improvement of reproductive traits is that recording the time for an animal to reach sexual maturity is costly. Another issue is that accurate estimates of breeding values are obtained only a long time after the young bulls have gone through selection. An alternative to overcome these problems is to use traits that are indicators of the reproductive efficiency of the herd and are easier to measure, such as age at first calving. Another problem is that heifers that have conceived once may fail to conceive in the next breeding season, which increases production costs. Thus, increasing heifer’s rebreeding rates should improve the economic efficiency of the herd. Response to selection for these traits tends to be slow, since they have a low heritability and phenotypic information is provided only later in the life of the animal. Genome-wide association studies (GWAS) are useful to investigate the genetic mechanisms that underlie these traits by identifying the genes and metabolic pathways involved.Results
Data from 1853 females belonging to the Agricultural Jacarezinho LTDA were used. Genotyping was performed using the BovineHD BeadChip (777 962 single nucleotide polymorphisms (SNPs)) according to the protocol of Illumina - Infinium Assay II ® Multi-Sample HiScan with the unit SQ ™ System. After quality control, 305 348 SNPs were used for GWAS. Forty-two and 19 SNPs had a Bayes factor greater than 150 for heifer rebreeding and age at first calving, respectively. All significant SNPs for age at first calving were significant for heifer rebreeding. These 42 SNPs were next or within 35 genes that were distributed over 18 chromosomes and comprised 27 protein-encoding genes, six pseudogenes and two miscellaneous noncoding RNAs.Conclusions
The use of Bayes factor to determine the significance of SNPs allowed us to identify two sets of 42 and 19 significant SNPs for heifer rebreeding and age at first calving, respectively, which explain 11.35 % and 6.42 % of their phenotypic variance, respectively. These SNPs provide relevant information to help elucidate which genes affect these traits. 相似文献45.
Eduardo?M?Del Aguila Marcio?B?Dutra Joab?T?Silva Vania?MF?PaschoalinEmail author 《BMC molecular biology》2005,6(1):9
Background
Preparation of RNA free from DNA is a critical step before performing RT-PCR assay. Total RNA isolated from several sources, including those obtained from Saccharomyces cerevisiae, using routine methodologies are frequently contaminated with DNA, which can give rise to amplification products that mimic the amplicons expected from the RNA target. 相似文献46.
Eduardo?S?SilvaEmail author Gerard?J?Schoone Celia?MF?Gontijo Reginaldo?P?Brazil Raquel?S?Pacheco Henk?DFH?Schallig 《Kinetoplastid biology and disease》2005,4(1):4
Background
The direct agglutination test (DAT) has proved to be a very important sero-diagnostic tool combining high levels of intrinsic validity and ease of performance. Otherwise, fast agglutination screening test (FAST) utilises only one serum dilution making the test very suitable for the screening of large populations. 相似文献47.
Alternative antigens reduce cross-reactions in an ELISA for the detection of Salmonella enteritidis in poultry 总被引:1,自引:0,他引:1
Two alternative antigens for the use in detection of antibodies to salmonellas were investigated: firstly, lipopolysaccharide (LPS) from members of the D2 group, having antigens O : 9, 46, and flagella antigens. Whereas LPS from the D2 group did not discriminate sufficiently with control sera, flagella antigens reacted specifically with antibodies directed to serotype specific H antigens. When flagella antigens were used to screen sera from birds of commercial flocks, however, cross-reactivity between flagella antigens was observed. When both LPS and flagella antigens were used to screen sera from chickens infected with Salmonella, enteritidis, the sera gave higher titres with flagella antigens during the early stages of infection, and titres with flagella antigens dropped earlier after infection had ended than titres with lipopolysaccharide. 相似文献
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Marc F. D. Baay Jitze M. Duk Klaas H. Groenier Matthé P. M. Burger Henk W. A. de Bruijn Harry Hollema Ernst Stolz P. Herbrink 《Cancer immunology, immunotherapy : CII》1997,44(4):211-215
To investigate the clinical significance of the enhanced sensitivity of antibody detection by radio immunoprecipitation assays
(RIPA), using in vitro translated HPV-16 E6 and E7 proteins, over synthetic-peptide enzyme-linked immunosorbent assay (ELISA),
RIPA for HPV-16 E6 and E7 were performed. The results obtained with E6 and E7 RIPA were related to clinico-pathological data
from cervical carcinoma patients positive for HPV type 16 DNA in their primary tumour. The data obtained with E6 and E7 RIPA
were then compared to the results obtained using the E7/6-35 synthetic-peptide ELISA. The prevalence of antibodies to E6,
E7, E6 and/or E7 and E6 and E7, as determined by RIPA, was significantly higher in cervical cancer patients than in both controls
and cervical intraepithelial neoplasia patients. Odds ratios, calculated for cervical carcinoma patients versus controls,
ranged from 7.4 to 15.4. Antibodies to E6 and/or E7 were largely restricted to patients with HPV DNA in their primary tumour.
Analysis of the relation between prevalence of antibodies to E6 and E7 and clinico-pathological parameters was limited to
85 patients positive for HPV-16 DNA. The strongest relation with clinico-pathological data, such as lesion size, lymph node
involvement, and prognosis, was found for E7 synthetic-peptide ELISA, whereas E6 and E7 RIPA did not reach significance. The
significance of these findings is discussed.
Received: 17 February 1997 / Accepted: 13 March 1997 相似文献
50.
Identification of the domain which determines the g,m serotype of the flagellin of Salmonella enteritidis. 总被引:1,自引:0,他引:1
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A J van Asten K A Zwaagstra M F Baay J G Kusters J H Huis in't Veld B A van der Zeijst 《Journal of bacteriology》1995,177(6):1610-1613
Clones expressing fragments of the flagellin protein of Salmonella enteritidis were constructed and screened with a g,m-specific monoclonal antibody. Results showed that the g,m epitope is localized between amino acids 258 and 348 of the flagellin. The fliC gene, encoding the flagellin of S. enteritidis, was proven to be the only flagellin gene present in S. enteritidis. 相似文献