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21.
BACKGROUND: Biochemical testing for pheochromocytoma by measurement of fractionated plasma metanephrines is limited by false positive rates of up to 18% in people without known genetic predisposition to the disease. The plasma normetanephrine fraction is responsible for most false positives and plasma normetanephrine increases with age. The objective of this study was to determine if we could improve the specificity of fractionated plasma measurements, by statistically adjusting for age. METHODS: An age-adjusted metanephrine score was derived using logistic regression from 343 subjects (including 33 people with pheochromocytoma) who underwent fractionated plasma metanephrine measurements as part of investigations for suspected pheochromocytoma at Mayo Clinic Rochester (derivation set). The performance of the age-adjusted score was validated in a dataset of 158 subjects (including patients 23 with pheochromocytoma) that underwent measurements of fractionated plasma metanephrines at Mayo Clinic the following year (validation dataset). None of the participants in the validation dataset had known genetic predisposition to pheochromocytoma. RESULTS: The sensitivity of the age-adjusted metanephrine score was the same as that of traditional interpretation of fractionated plasma metanephrine measurements, yielding a sensitivity of 100% (23/23, 95% confidence interval [CI] 85.7%, 100%). However, the false positive rate with traditional interpretation of fractionated plasma metanephrine measurements was 16.3% (22/135, 95% CI, 11.0%, 23.4%) and that of the age-adjusted score was significantly lower at 3.0% (4/135, 95% CI, 1.2%, 7.4%) (p < 0.001 using McNemar's test). CONCLUSION: An adjustment for age in the interpretation of results of fractionated plasma metanephrines may significantly decrease false positives when using this test to exclude sporadic pheochromocytoma. Such improvements in false positive rate may result in savings of expenditures related to confirmatory imaging. 相似文献
22.
JONATHAN B. COHEN SARAH M. KARPANTY JAMES D. FRASER BRYAN D. WATTS BARRY R. TRUITT 《The Journal of wildlife management》2009,73(6):939-945
ABSTRACT Weekly counts of western Atlantic red knots (Calidris canutus rufa) at their Delaware Bay migration stopover site have suggested a major decline since the 1980s. We estimated red knot spring passage population size in the New Jersey Coast-Delaware Bay region (DENJ; 2004 and 2006) and Virginia (VA; 2006 and 2007), USA, by correcting weekly aerial counts for mean daily residence probability between counts in a Monte-Carlo simulation. We used daily telemetry relocations in mark-resight models to estimate mean daily residence probability. Average daily residence probability was approximately 1.0 in mid-May, 0.96–0.97 in the week of 22 May, and 0.64–0.77 after May 28 in DENJ in 2004 and 2006 and in VA in 2006. Average daily residency was approximately 0.88 in VA in 2007 from 22 May to 5 June. No birds moved from VA to DENJ in 2006 and only 2 birds (5.5%) moved in 2007. Stopover population sizes (±SE) in DENJ were 17,108 ± 1,322 in 2004 and 19,555 ± 831 in 2006, and in VA were 7,224 ± 389 in 2006 and 8,332 ± 718 in 2007, significantly greater than peak aerial counts. Years with similar peak counts had different residence probabilities; hence, adjustments for turnover should be used in the future to assess annual population changes. Our results suggest that VA can support a significant portion of this red knot subspecies during migration in at least some years. Managing red knots for recovery should entail improving our understanding of the use of other Atlantic Coast sites and protecting key coastal habitat from disturbance and development. 相似文献
23.
DUANE R. DIEFENBACH MARY JO CASALENA MICHAEL V. SCHIAVONE DAVID SWANSON MICHAEL REYNOLDS ROBERT C. BOYD ROBERT ERIKSEN BRYAN SWIFT 《The Journal of wildlife management》2009,73(6):996-999
ABSTRACT We estimated loss of butt-end leg bands on male wild turkeys (Meleagris gallapavo) captured in New York, Ohio, and Pennsylvania (USA) during December-March, 2006–2008. We used aluminum rivet leg bands as permanent marks to estimate loss of regular aluminum, enameled aluminum, anodized aluminum, and stainless steel butt-end leg bands placed below the spur. We used band loss information from 887 turkeys recovered between 31 days and 570 days after release (x̄ = 202 days). Band loss was greater for turkeys banded as adults (>1 yr old) than juveniles and was greater for aluminum than stainless steel bands. We estimated band retention was 79–96%, depending on age at banding and type of band, for turkeys recovered 3 months after release. Band retention was <50% for all age classes and band types 15 months after banding. We concluded that use of butt-end leg bands on male wild turkeys is inappropriate for use in mark-recapture studies. 相似文献
24.
Fatty acid synthesis from [14C]acetate was studied in the brownalgae, Fucus serratus and Fucus vesiculosus, which had beencollected from sites polluted by run-off from old copper mines.Algae collected from areas exposed to copper in situ showedchanges in the pattern of fatty acids labelled in vitro withan increase in oleate labelling and a decrease in palmitatelabelling in both species. The endogenous fatty acid patternsfor algae from polluted sites also differed from those fromunpolluted sites. Algae from sites polluted by copper containedmore oleate than samples from non-polluted sites. In addition,there were decreases in the polyunsaturated fatty acids -linolenate,octadecatetraenoate and docosapentaenoate for copper-exposedalgae. These differences are discussed with regard to the possiblemetabolic site of copper's action and to the relative toleranceof Fucus spp. to high environmental dissolved copper levels. Key words: Fucus spp., Copper pollution, Fatty acid synthesis 相似文献
25.
Short-Term In Vitro Culture and Molecular Analysis of the Microsporidian, Enterocytozoon bieneusi 总被引:2,自引:0,他引:2
GOVINDA S. VISVESVARA GORDON J. LEITCH NORMAN J. PIENIAZEK ALEXANDRE J. DA SILVA SARA WALLACE SUSAN B. SLEMENDA RAINER WEBER DAVID A. SCHWARTZ LEO GORELKIN C. MEL WILCOX RALPH T. BRYAN 《The Journal of eukaryotic microbiology》1995,42(5):506-510
ABSTRACT. The microsporidium, Enterocytozoon bieneusi , causes a severe, debilitating, chronic diarrhea in patients with the acquired immunodeficiency syndrome. Specific diagnosis of intestinal microsporidiosis, especially due to Enterocytozoon , is difficult and there is no known therapy that can completely eradicate this parasite. Preliminary studies indicate that a short term (about 6 months) in vitro culture of this parasite yielding low numbers of spores, may be established by inoculating human lung fibroblasts and/or monkey kidney cell cultures with duodenal aspirates and or biopsy from infected patients. The cultures may subsequently be used for the isolation and molecular analysis of parasite DNA. 相似文献
26.
GEORGINA BRYAN 《Ecological Entomology》1983,8(3):259-270
ABSTRACT.
- 1 Most Achrysocharoides species and their Phyllonorycter hosts (Lepidoptera, Gracillariidae) have two generations per year in Britain.
- 2 In those species with separate sex broods, peak male emergence tends to be earlier than peak female emergence. This female emergence lag is shorter in the second generation.
- 3 The mean brood size in the second generation is significantly smaller than in the first in A.cilla males and females, A.latreilli females, mixed sex broods of A.atys, and A.carpini females. A.cilla, A.latreilli and A.niveipes generally have a significantly greater proportion of males in the second generation, but A.atys does not.
- 4 There is a shift to killing later instar Phyllonorycter larvae in the second generation, when a much higher percentage parasitism is generally achieved.
- 5 The intergeneration differences in sex ratio and brood size may be explained by a change in oviposition behaviour of females of the first and second generations.
27.
Abstract.
- 1 Seasonal reproductive development was investigated in four obligate fungivorous species, D.phalerata Meigen, D.transversa Fallen, D.cameraria Haliday and D.confusa Staeger.
- 2 Samples of females taken from woodlands over a 2 year period were scored for reproductive status and fat content.
- 3 Populations of D.phalerata and D.cameraria maintained in an outdoor insectary provided information on developmental time and adult mortality throughout the year.
- 4 Experiments in controlled environmental chambers gave percentage diapause curves against day length for all four species.
- 5 In D.phalerata the influence of temperature and fungus upon diapause behaviour was also investigated.
- 6 In D.phalerata the optimal strategy for reproductive development can vary from month to month. Maturation of females may be immediate, dependent upon a fungal cue or completely prevented.
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