光动力疗法治疗感染性疾病的动物模型

光动力疗法(photodynamic therapy,PDT)是利用特定波长的激发光照射生物靶标上的光敏剂,从而产生活性氧并有效杀伤多种耐药病原体的新型治疗方式,具有作用广、安全可控、不易耐受等优点。大量体外实验已证实了PDT疗效,但目前动物实验数据较少,且治疗参数不一,一定程度上影响了PDT在临床治疗中的广泛应用。本文综述近年来PDT用于体内抗感染治疗的动物模型构建、治疗方案设计等方面的研究进展,为未来PDT抗感染研究及临床应用提供参考。     

小脑间位核和顶核对下丘脑外侧区神经元电活动的影响

电刺激猫小脑问位核和顶核可以影响下丘脑外侧区神经元的电活动,其中有一些神经元是葡萄糖敏感神经元.这一结果揭示小脑不仅具有经典的躯体运动调节功能,同时也可以通过小脑-下丘脑通路参与机体非躯体活动的调节.     

外源碳输入对中亚热带森林深层土壤碳矿化和微生物决策群落的影响

在陆地生态系统中,深层土壤是重要的有机碳库.外源碳输入可改变土壤有机碳(SOC)矿化速率(激发效应),进而影响土壤碳排放.然而深层土壤对外源碳输入的响应程度和方向如何还不清楚,引起激发效应的机理尚不明确.本文利用¹³C标记葡萄糖添加试验,分析亚热带森林不同层次SOC矿化的激发作用,并通过微生物决策群落(r-K策略者)的相对变化来探讨激发效应的机理.结果表明: 深层土壤矿化速率显著低于表层土壤,添加标记葡萄糖后能增加所有层次土壤原有SOC的矿化(正激发效应),但是深层土壤的激发效应强度(156%)显著高于表层土壤(45%).葡萄糖添加显著降低了各层次土壤微生物的最大比生长速率,表明r策略者相对比例下降而K策略者相对比例增加.推测SOC矿化的正激发效应主要由K策略者的相对比例变化引起.此外,葡萄糖添加后可溶性有机碳和可溶性氮的比值在深层土壤中(76.03)显著高于表层土壤(13.00),暗示深层土壤存在更为强烈的氮限制作用.深层土壤微生物为获取氮源,可能会加剧对原有SOC的矿化,进而产生更强烈的激发效应.深层土壤SOC矿化受碳源和氮源的限制,更容易受外源碳输入的影响,对未来气候变化也更敏感.     

一种新的硒酶——硫氧还蛋白还原酶

必需微量元素硒的生物功能主要是通过各种硒酶和硒蛋白来实现的,如谷胱甘肽过氧化物酶（ＧＰｘ）类具有抗氧化功能,碘甲腺原氨酸脱碘酶（ＤＩ）类在甲状腺激素的生物合成和代谢中具有重要作用。已有研究表明,在哺乳动物各种组织中可能大约存在２５种左右硒蛋白,但是迄...     

A novel receptor-targeted gene delivery system for cancer gene therapy

Some growth factor receptors, such as insulin like growth factor Ⅰ and Ⅱ receptor (IGF Ⅰ R, IGF Ⅱ R) and epidermal growth factor receptor (EGF R), have been proved to be over-expressed in a variety of human cancers derived from different tissue origins. Based on this molecular alteration, a polypeptide conjugate gene delivery system was designed and synthesized. It contains three essential moieties: a ligand oligopeptide (LOP) for receptor recognition, a polycationic polypeptide (PCP) such as protamine (PA) or poly-L-lysine (PL) as a backbone for DNA binding and an endosome-releasing oligopeptide (EROP) such as influenza baenagglutinin oligopeptide (HA20) for endosomolysis. These components are covalently conjugated as LOP-PCP-HA20 or in the form of a mixture of LOP-PCP and HA20-PCP. A 14 amino acid E5 was designed and synthesized as LOP for IGF Ⅰ R and IGF Ⅱ R, and a 16 amino acid GE7 as LOP for EGF R. Both E5 and GE7 systems could form stable complex with the plasmid DNA as E5-PCP/DNA/PCP-HA20 a     

The phylogenetic relationships among the possible donors of B-genome of common wheat based on internal transcribed spacer (ITS) sequences

The ITS regions of 5 species in Aegilops sect. Sitopsis, the possible donors of Bgenome of common wheat, were amplified by PCR, cloned and sequenced. The phylogenetic relationships among 5 species in Aegilops sect. Sitopsis were constructed based on ITS1 + ITS2 sequences. The results demonstrated that Ae. speltoides was a distinct species in Aegilops sect. Sitopsis. The average of the pairwise distances between Ae. speltoides and the other four species was three times as high as that among the latter four. Ae. speltoides was the earliest lineage of the section under question. Relationship between Ae. longissima and Ae. sharonensis was the closest in Aegilops sect. Sitopsis. Sequence of ITS regioncould be used as a molecular marker to identify origin of B-genome in polyploid wheats.     

内蒙古准格尔旗对臼沟本溪组及腕足动物群落

介绍了内蒙古准格尔旗对臼沟本溪组含腕足动物地层,对该地区本溪组腕足动物群和性质特点进行了分析。并在识别腕足动物群落的基础上,从种的分异度,种的相对丰度,群落空间分布与能量分配等角度探讨了该腕足动物群落的一些结构模式,指出Ｅｃｈｉｎｏｃｏｃｈｕｓ ｅｌｅｇａｎｓ－Ｏｈｏｒｉｓｔｉｔｅｓ ｗｅｉｎｉｎｇｅｎｓｉｓ腕足动物群落已基本属于演替阶段中的顶极群落。