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71.
Summary Three possible explanations of the negative capacitance seen in theChara corallina membrane impedance are critically examined. These explanations are based on: (1) voltage-dependent channel kinetics; (2) electro-osmosis; and (3) extracellular negative capacitance. It is shown that the first two can produce negative capacitance only with parameters which differ by several orders of magnitude from measured values. The last mechanism can produce a very large magnitude negative capacitance, in the appropriate frequency range. Possible experimental tests are discussed. 相似文献
72.
Dr. Corine Layet Terry Delovitch Pierre Ferrier Danielle H. Caillol Bertrand R. Jordan François A. Lemonnier 《Immunogenetics》1985,21(5):469-478
Radioimmunoassay of HLA-transformed mouse L cells expressing A3, A24, B7, or Cw3 HLA class I molecules with a set of monomorphic monoclonal antibodies distinguishes between A3–A24 and B7-Cw3 patterns of reactivity. Analyses with Bw6-specific monoclonal antibodies and a human alloantiserum demonstrate the expression by the HLA-Cw3 molecules of a Bw6 public specificity related to but not identical with that expressed by the HLA-B7 molecules. Exon-shuffling experiments and inhibition studies of monoclonal antibody cell-surface fixation indicate that similar parts of B7 and Cw3 molecules account for their serological cross-reactivity.Abbreviations used in this paper
2-m
beta-2 microglobulin
- EBV
Epstein-Barr virus
- mAb
monoclonal antibody
- PBS-BSA
phosphate-buffered saline supplemented with 0.2% bovine serum albumin
- PBL
peripheral blood lymphocyte
- RIA
radioimmunoassay 相似文献
73.
74.
Altered T cell development in mice with a targeted mutation of the CD3-epsilon gene. 总被引:21,自引:2,他引:19
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M Malissen A Gillet L Ardouin G Bouvier J Trucy P Ferrier E Vivier B Malissen 《The EMBO journal》1995,14(19):4641-4653
75.
Time-dependent Behavior of a Mathematical Model for Munch Translocation: Application to Recovery from Cold Inhibition 总被引:1,自引:1,他引:0
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Ferrier JM 《Plant physiology》1975,55(3):511-514
The time evolution of a Munch pressure-flow translocation system is calculated using a numerical computer method. Results are obtained for the time course of the system variables following application of a large resistance in the translocation path, intended to simulate a cold block. The resistance factor required to produce translocation inhibition indicates that even moderate inhibition is primarily due to sieve plate pore block-age, rather than to the viscosity increase. The calculated time for recovery from cold inhibition and the shape of the translocation recovery curve agree with experimental results. The time for translocation recovery and the level of velocity recovery depend on the rate of sucrose unloading in the sink; on the sucrose concentration in the sieve tube; on the position, length, and resistance factor of the cold block; and on the hydraulic conductivities. 相似文献
76.
A simpler iterative steady state solution of münch pressure-flow systems applied to long and short translocation paths
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A simple steady state iterative solution of Münch pressure-flow in unbranched sieve tubes containing only water and sucrose is derived. The iterative equations can be solved on a programmable desk calculator. Solutions are presented for steady state transport with specific mass transfer rates up to 1.5 × 10−5 mole second−1 centimeters−2 (= 18.5 grams hour−1 centimeters−2) over distances in excess of 50 meters. The calculations clearly indicate that a Münch pressure-flow system can operate over long distances provided (a) the sieve tube is surrounded by a semipermeable membrane; (b) sugars are actively loaded in one region and unloaded at another; (c) the sieve pores are unblocked so that the sieve tube hydraulic conductivity is high (around 4 centimeters2 second−1 bar−1); (d) the sugar concentration is kept high (around one molar in the source region); and (e) the average sap velocity is kept low (around 20-50 centimeters hour−1). The dimensions of sieve cells in several species of plants are reviewed and sieve tube hydraulic conductivities are calculated; the values range from 0.2 to 20 centimeters2 second−1 bar−1. For long distance pressure-flow to occur, the hydraulic conductivity of the sieve cell membranes must be about 5 × 10−7 centimeters second−1 bar−1 or greater. 相似文献
77.
Subcellular Pathology of Human Neurodegenerative Disorders: Alzheimer-Type Dementia and Huntington''s Disease 总被引:1,自引:1,他引:0
A. J. Cross T. J. Crow J. M. Dawson I. N. Ferrier J. A. Johnson T. J. Peters G. P. Reynolds 《Journal of neurochemistry》1986,47(3):882-889
Activities of enzyme markers of subcellular organelles have been measured in brain tissue from subjects with Alzheimer-type dementia (ATD) and Huntington's disease (HD). Significant increases in the activity of the lysosomal enzyme beta-glucuronidase were observed in both ATD temporal cortex and HD putamen. It is suggested that beta-glucuronidase activity may be a useful biochemical indicator of cellular damage in the CNS. A significant reduction in neutral alpha-glucosidase activity was observed in ATD temporal cortex and HD putamen. This change may reflect an alteration in glycoconjugate processing and may relate to the susceptibility of neurones to the degenerative processes of ATD and HD. 相似文献
78.
Régis Sodoyer Brigitte Kahn-Perles Torn Strachan Joséphine Sire Marie-José Santoni Corine Layet Pierre Ferrier Bertrand R. Jordan Francois A. Lemonnier 《Immunogenetics》1986,23(4):246-251
The individual contributions of the first two external domains of the HLA-B7 heavy chain to the expression of allele-specific (B7) and locus-specific (B and C) antigenic determinants were investigated using hybrid class I genes. Hybrid genes were constructed in vitro by exon shuffling between the parent genes HLA-B7, HLA-Cw3, HLA-A3, and H-2K
d, and their expression was monitored following transfection into mouse L cells. The results show that most allele-specific antigenic determinants are associated with the first external domain of the 137 heavy chain, whereas all the locus-specific antigenic determinants tested map to the second external domain.Abbreviations used in this paper BSA
bovine serum albumin
- FCS
fetal calf serum
- mAb
monoclonal antibody
- PBL
peripheral blood lymphocytes
- PBS
phosphate-buffered saline 相似文献
79.
J. Hughlings Jackson Thomas Buzzard R. Brudenell Carter H. Charlton Bastian W. R. Gowers David Ferrier J. A. Ormerrod Charles E. Beevor Howard H. Tooth James Taylor J. S. Risien Russell W. Aldren Turner Frederick E. Batten Felix Semon Victor Horsley Charles A. Ballance R. Marcus Gunn A. E. Cumberbatch 《BMJ (Clinical research ed.)》1900,2(2066):333
80.
Serotonin Receptor Changes in Dementia of the Alzheimer Type 总被引:11,自引:6,他引:5
A. J. Cross T. J. Crow I. N. Ferrier J. A. Johnson S. R. Bloom J. A. N. Corsellis 《Journal of neurochemistry》1984,43(6):1574-1581
Serotonin receptors were assessed in post-mortem brains of control and Alzheimer-type dementia (ATD) patients using ligand binding techniques. Differential losses of serotonin S1 and S2 receptors were present in neocortex, hippocampus, and amygdala of ATD patients, whereas no significant changes were observed in basal forebrain and basal ganglia. Losses of S1 receptors were significantly age-related in the ATD group, suggesting they occurred at a later stage of the disease process. Losses of S2 receptors were considerably greater (with a reduction to 35% of control in temporal cortex) and were not age-related in ATD. Significant correlations were observed within the ATD group between S2 receptor binding and somatostatin immunoreactivity in temporal and frontal cortices. Thus the loss of S2 receptors in ATD may be a relatively early change in the disease process, and may precede the changes in ascending serotonergic neurones. 相似文献