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161.
ANDALO CHRISTOPHE; RAQUIN CHRISTIAN; MACHON NATHALIE; GODELLE BERNARD; MOUSSEAU MARIANNE 《Annals of botany》1998,81(3):405-411
Individuals ofArabidopsis thaliana, collected in different naturalpopulations, were grown in controlled and elevated CO2in a glasshouse.Following germination, root growth of progeny of different linesof these populations was studied in control and elevated atmosphericCO2. No significant direct effect of atmospheric CO2concentrationcould be demonstrated on root growth. An important parentaleffect was apparent, namely that root length and branching weredecreased in seeds collected from a mother plant which had beengrown in elevated CO2. This was correlated with smaller seeds,containing less nitrogen. These parental effects were geneticallyvariable. We conclude that CO2may affect plant fitness via parentaleffects on seed size and early root growth and that the geneticvariability shown in our study demonstrates thatArabidopsispopulationswill evolve in the face of this new selective pressure.Copyright1998 Annals of Botany Company Root growth, root branching, seed, elevated CO2, natural population,Arabidopsis thaliana, parental effect. 相似文献
162.
MARIE CHABOT-FLETCHER MAUREEN L. HO JOHN J. BRETON CHARLES R. HANNING BERNARD Y. AMEGADZIE 《Cell biology international》1996,20(11):777-779
A recombinant baculovirus was constructed to express a cDNA encoding RelA (p65), a member of the NF-κB/Rel family of proteins. Infection of Spodoptera frugiderda insect cells with the recombinant baculovirus resulted in the production of the biologically active protein as measured by immunoblotting using RelA-specific antisera and by electrophoretic mobility shift assays. The recombinant protein bound specifically to an oligonucleotide containing the NF-κB consensus motif but not to that containing the unrelated Oct-1 consensus motif. Thus insect cell-derived RelA possess properties similar to the native protein and may be used in physical, biochemical, and pharmacological studies. 相似文献
163.
New aspects of rubber biosynthesis 总被引:5,自引:0,他引:5
BERNARD L. ARCHER BRAIN G. AUDLEY 《Botanical journal of the Linnean Society. Linnean Society of London》1987,94(1-2):181-196
New aspects of rubber biosynthesis. Following a review of the site of rubber biosynthesis in Hevea brasiliensis and Parthenium argentalum, evidence is given for the initiation of polyisoprene molecules from (ranMerpenoid precursors including geranylgeranyl pyrophosphate. All franj-14 C-geranylgeraniol has been isolated from incubations of H. brasiliensis latex serum with 14 C-isopentenyl pyrophosphate. Gel-filtration chromatography of the serum yields very small rubber particles of high biosynthetic activity, and two proteinaceous fractions. One of these increases the biosynthesis of rubber and may contain the enzyme, isopentenyldiphosphate δ-isomerase, whilst the other appears to inhibit rubber formation. The nature and molecular weight of the rubber formed in vitro is discussed and a mechanism for the de novo formation of rubber particles is suggested. 相似文献
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166.
BERNARD THOIRON ARLETTE THOIRON JEANNE LE GUIEL ULRICH LüTTGE MICHEL THELLIER 《Physiologia plantarum》1979,46(4):352-356
When the ambient atmosphere of Acer pseudoplatanus cells in suspension culture is rapidly changed by opening the culture flasks and gently stirring (‘mild gas-shock’) or by filtering and suspending in new medium (‘strong gas-shock’), drastic modifications of the rates of leucine, methionine, glucose, adenine, sulphate and phosphate uptake are observed. Following the gas-shock, rates of uptake rapidly decrease within a few minutes. Subsequently the rates increase again to the intial level within several hours. The uptake of potassium, which is known to be passively distributed between the medium and the interior of many plant cells, at least at high external concentrations, is apparently independent of gas-shock. The shock and recovery kinetics are similar for all solutes investigated (except K+), in particular for different solutes studied in double labelling experiments with the same batch of cells. At the maximum of the after-effect of shock, i.e. at minimum rates of uptake, uptake shows a highly reduced dependence on temperatures. Gas-shock probably inactivates, denatures, structurally alters or releases membrane macromolecules engaged in transport. These molecules are then re-synthesized and re-incorporated into the membrane during recovery. 相似文献
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168.
GEORGES HEMERY FRANK D'AMICO† IKER CASTEGE BERNARD DUPONT‡ JEAN D'ELBEE§ YANN LALANNE† CLAUDE MOUCHES† 《Global Change Biology》2008,14(1):27-38
Large‐scale univariate climate indices (such as NAO) are thought to outperform local weather variables in the explanation of trends in animal numbers but are not always suitable to describe regional scale patterns. We advocate the use of a Multivariate Oceanic and Climatic index (MOCI), derived from ‘synthetic’ and independent variables from a linear combination of the total initial variables objectively obtained from Principal Component Analysis. We test the efficacy of the index using long‐term data from marine animal populations. The study area is the southern half of the Bay of Biscay (43°–47°N; western Europe). Between 1974 and 2000 we monitored cetaceans and seabirds along 131000 standardized line transects from ships. Fish abundance was derived from commercial fishery landings. We used 44 initial variables describing the oceanic and atmospheric conditions and characterizing the four annual seasons in the Bay of Biscay. The first principal component of our MOCI is called the South Biscay Climate (SBC) index. The winter NAO index was correlated to this SBC index. Inter‐annual fluctuations for most seabird, cetacean and fish populations were significant. Boreal species (e.g. gadiformes fish species, European storm petrel and Razorbill …) with affinities to cold temperate waters declined significantly over time while two (Puffin and Killer Whale) totally disappeared from the area during the study period. Meridional species with affinities to hotter waters increased in population size. Those medium‐term demographic trends may reveal a regime shift for this part of the Atlantic Ocean. Most of the specific observed trends were highly correlated to the SBC index and not to the NAO. Between 40% and 60% of temporal variations in species abundance were explained by the multivariate SBC index suggesting that the whole marine ecosystem is strongly affected by a limited number of physical parameters revealed by the multivariate SBC index. Aside the statistical error of the field measurements, the remaining variation unexplained by the physical characteristics of the environment correspond to the impact of anthropogenic activities such overfishing and oil‐spills. 相似文献
169.
DAWIT T. DEGEFU BRETT P. HURLEY JEFF GARNAS MICHAEL J. WINGFIELD RODRIGO AHUMADA BERNARD SLIPPERS 《Ecological Entomology》2013,38(1):112-116
1. Two cossid moths, Coryphodema tristis Drury and Chilecomadia valdiviana Philippi, have recently become pests on Eucalyptus nitens H. Deane & Maiden in South Africa and Chile, respectively. Both C. tristis and C. valdiviana have large host ranges and high levels of similarity in their host distributions. Their infestations of E. nitens are the first records of these moths on Myrtaceae. 2. The contemporaneous adoption of E. nitens as a novel host, despite widespread availability of native and introduced Myrtaceae, suggests a non‐random pattern of invasion. Phylogenetic relatedness among the two species linked to cryptic invasion of one or both moths at some time in the recent past provides a possible explanation for this pattern. 3. To test this hypothesis, variation in mtDNA sequences for the COI gene of C. tristis and C. valdiviana were analyzed. The COI mtDNA sequence data showed that C. tristis and C. valdiviana are highly divergent genetically, indicating that both are native on their respective continents with independent evolutionary trajectories. 4. The parallel host range expansions to E. nitens on different continents appear to be unrelated events, likely driven by characteristics of the biology and/or ecology of the host. 相似文献
170.
Analyses of morphological data on the higher-level phylogenetics of Thysanoptera have suggested two alternative hypotheses: (1) sister-taxon status of the monophyletic suborders Terebrantia and Tubulifera. and (2) paraphyly of Terebrantia with respect to Tubulifera. Analyses of nucleotide sequence data from the mitochondrial gene cytochrome oxidase I and the nuclear gene I8S rONA using maximum parsimony. neighbour joining. and maximum likelihood provide strong support for the hypothesis of a sister-taxon relationship between the Terebrantia and Tubulifera. These data resolve this long-standing controversy in thysanopteran phylogenetics and provide a framework for further studies of higher-level relationships in this order. 相似文献