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Summary. Fast protein liquid chromatography (FPLC) and polyacrylamide gel electrophoresis were used to determine the ratio between I α-and II α-globin chains in Norwegian dairy goats. Three different phenotypes, designated normal (N) with I α- to II α-globin ratio 3:1, reversed (R) with ratio 1:2 and double-reversed (RR) with no I α-globin, were described. Family studies indicated that the R animals were heterozygous, and the RR animal homozygous, for a haplotype without a functional I α-globin product. Southern blot analysis of goat DNAs digested with six different restriction enzymes showed that the different ratios of α chain expression could not be due to a deletion of the I α-gene and/or duplication or triplication of the II α-globin genes. The homozygous reversed animal with no detectable I α-globin had a mild anaemia. 相似文献
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Methylmethacrylate (MMA) is the most commonly used embedding medium for sectioning undecalcified bone; however, a number of problems exist with its use in a research laboratory. MMA requires a long infiltration time and temperature control, and it reacts with many polymers. We used Kleer Set resin™ as an alternative embedding medium for sectioning undecalcified bone specimens. Fluorochrome labeled bone specimens were sectioned transversely using a ground section technique and longitudinally on a sledge macrotome. The slides were viewed using both transmitted light and epifluorescence microscopy. High quality sections were obtained using Kleer Set resin™ for both sectioning techniques. We have shown that this new embedding medium is simpler, safer, quicker to use and does not interfere with visualization of fluorochromes. 相似文献
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Restriction fragment length polymorphism (RFLP) analysis was used to characterize the beta-globin cluster in Norwegian dairy goats. In 122 individuals, different RFLP patterns were detected after BglII and PstI digestion and hybridization with a caprine beta-globin probe. The location of the polymorphic BglII and PstI sites were determined. The different RFLPs were in linkage disequilibrium with each other and with the beta-globin locus as studied at the protein level. A preferential association between certain RFLP haplotypes and beta-globin variants was observed. Polymorphic DNA fragments in the epsilon-globin genes were detected after MspI digestion and hybridization with a caprine epsilon-probe, and eight different band patterns were observed. Correlation between different MspI haplotypes and the beta-globin variants was determined. 相似文献
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