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141.
Species delimitation, an issue central to systematics and biodiversity studies, is addressed in the epunctifera group of the stem borer genus Sesamia Guenée (Lepidoptera: Noctuidae). This group is composed of four sub‐Saharan species: Sesamia poephaga Tams & Bowden; Sesamia epunctifera Hampson; Sesamia penniseti Tams & Bowden; and Sesamia poebora Tams & Bowden, the taxonomic status of which was unclear. The first species was considered a possible synonym of the second, and the third species was considered a possible synonym of the fourth. An analysis combining morphological, ecological and molecular data enables us to conclude that S. epunctifera and S. poephaga are different species, and that S. poebora is a synonym of S. penniseti. Two new species were discovered: Sesamia firmata sp.n. and Sesamia veronica sp.n. Sesamia firmata sp.n. has atypical genitalic morphology, suggesting a strong selection resulting in a reinforcement of pre‐zygotic isolation. Some specimens previously identified as S. penniseti on the basis of morphology are sisters to S. epunctifera on the mitochondrial tree, and are connected to S. penniseti on the nuclear tree. The mitochondrial distance from S. penniseti and S. epunctifera is 7.6% and 3.9%, respectively, suggesting an ancient mitochondrial introgression from S. epunctifera into S. penniseti. The possible causes of the reinforcement and introgressive hybridization are discussed. This case of mitochondrial introgression, uncommon in Lepidoptera, in which females are the heterogametic sex, may be an exception to Haldane's rule. The hybrid is assigned the rank of species and named Sesamia pennipuncta sp.n.  相似文献   
142.
Repression of competition (RC) within social groups has been suggested as a key mechanism driving the evolution of cooperation, because it aligns the individual’s proximate interest with the interest of the group. Despite its enormous potential for explaining cooperation across all levels of biological organization, ranging from fair meiosis, to policing in insect societies, to sanctions in mutualistic interactions between species, there has been no direct experimental test of whether RC favours the spread of cooperators in a well‐mixed population with cheats. To address this, we carried out an experimental evolution study to test the effect of RC upon a cooperative trait – the production of iron‐scavenging siderophore molecules – in the bacterium Pseudomonas aeruginosa. We found that cooperation was favoured when competition between siderophore producers and nonsiderophore‐producing cheats was repressed, but not in a treatment where competition between the two strains was permitted. We further show that RC altered the cost of cooperation, but did not affect the relatedness among interacting individuals. This confirms that RC per se, as opposed to increased relatedness, has driven the observed increase in bacterial cooperation.  相似文献   
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A method is presented that will give comparable results for the evaluation of electrophoretic data on hemolyzed mouse serum patterns. Not only may hemoglobin be determined in the serum, but its contribution to the total protein may be calculated with the aid of a reference curve derived from the data obtained from the hemolyzed red cells of mice taken from the corresponding mouse blood pool.  相似文献   
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GAGIANAS  A. A.; BERG  A. R. 《Annals of botany》1977,41(6):1135-1148
The effect of morphactin (methyl 2-chloro-9-hydroxyfluorene-9-carboxylate)on basipetal transport of auxin (Indol-3-ylacetic acid-2-14C)was studied in bean (Phaseolus vulgaris) hypocotyl with thedonor-receiver block method. Morphactin (5 x 10–6m) reduced IAA (5 x 10–6m) transportintensity by an average of 83 per cent and auxin transport capacityby 90 per cent, but transport velocity was not affected. Morphactin did not inhibit uptake of IAA into hypocotyl tissue,but it did prevent transfer of IAA from the tissue into receiverblocks. Chromatographic analysis of the tissue after 4 h IAA-2-14Ctransport showed that 54 per cent of the total activity wasin the form of IAA in the control and 42 per cent in the morphactintreated tissue. No difference was found in the rate of decarboxylationof IAA-1-14C between control and morphactin treated tissue sections.Nor could any difference between control and morphactin be shownin the radioactivity associated with a TCA ppt fraction. Ina study of the transportable auxin pool, morphactin decreasedthe size of the pool and increased the half-life of decay ofauxin transport from 1•22 h to 3•85 h. In a kineticanalysis of the reversal of morphactin (5 x 10–6m) inhibitionby increasing concentration of IAA-2-14C (5 x 10–6m to2 x 10–5m), it was shown that IAA transport resemblesMichaelis-Menten enzyme reaction kinetics, and that inhibitionby morphactin fitted a ‘mixed type’ model. IAA hada dissociation constant of 8•5 x 10–6m and morphactinthat of 4•3 x 10–7m with a Km for the transport processof 8•5 x 10–6m.  相似文献   
150.
A study of the development of methanogenic fixed films on pieces of polyvinyl chloride plastic, etched glass and baked clay showed that support material markedly affected the rate of attachment and growth of bacteria converting acetic acid to methane. Film development, as indicated by the rate of acetate conversion to methane and carbon dioxide, was threefold faster on fired clay than on either PVC plastic or etched glass. Scanning electron micrographs showed that the film of bacteria attached to clay was thick and uniform, while the film attached to PVC plastic was thin although still uniform. Attachment to etched glass was spotty. The characteristics of clay which made it a superior support appeared to be its rough, porous surface which offered attachment sites to the micro-organisms and the presence of minerals in the clay, particularly iron which is known to stimulate methanogenesis and growth.  相似文献   
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