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21.

Background  

Cloning of cattle by somatic cell nuclear transfer (SCNT) is associated with a high incidence of pregnancy failure characterized by abnormal placental and foetal development. These abnormalities are thought to be due, in part, to incomplete re-setting of the epigenetic state of DNA in the donor somatic cell nucleus to a state that is capable of driving embryonic and foetal development to completion. Here, we tested the hypothesis that DNA methylation patterns were not appropriately established during nuclear reprogramming following SCNT. A panel of imprinted, non-imprinted genes and satellite repeat sequences was examined in tissues collected from viable and failing mid-gestation SCNT foetuses and compared with similar tissues from gestation-matched normal foetuses generated by artificial insemination (AI).  相似文献   
22.
BULMER1 has analysed data on multiple allelic series of genes determining enzymes and has shown that if the alleles are arranged in order of electrophoretic mobility, there is a marked tendency for the rare alleles to occur at the beginning or the end of a sequence and for the common alleles to occur in the middle of a sequence.  相似文献   
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