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901.
902.
903.
An increasing incidence of sex-chromosome variation in constitutive heterochromatin, including individuals with mosaic genotypes, has been observed in a single natural population of Nesokia indica, the Indian mole rat. Variations in the heterochromatic areas of the X chromosome are largely due to deletions at R-band-positive regions corresponding to folate-sensitive fragile sites. All individuals with either a pre- or post-zygotic loss or gain of sex-chromosome heterochromatin have so far proved to be infertile. Whether such F1 sterility is due to abnormal gonadal development, gametic incompetence, or other factors is not clear. More important is the indication that the constitutive heterochromatin of this species may contain coding DNA sequences with putative regulatory functions. 相似文献
904.
Recent data have demonstrated that differences in sIg density on B lymphocytes distinguish functionally distinct subpopulations of these cells. Other reports suggest that cyropreservation may change the frequency of sIg-bearing lymphocytes. To determine if cryopreservation alters either the frequency of sIg cells or the distribution of sIg density, PBM from normals and patients with CLL and LCL were analyzed using the FACS. Aliquots of Ficoll-Hypaque-separated PBM were controlled-rate frozen (1 °C/min) in 7.5% Me2SO in RPMI 1640 and thawed in a 37 °C water bath on the same day. Fresh and frozen-thawed PBM aliquots were labeled with fluorescein conjugates of F(ab′) fragments of affinity chromatography-purified anti-Fab or class-specific anti-μ, anti-δ, anti-γ, or anti-α. Histograms of relative cell fluorescence, reflecting sIg density, were prepared for each aliquot with the FACS. The frequency of sIg-bearing PBM labeled with each reagent was not significantly altered by freezing. Likewise, FACS profiles demonstrated that the distribution of sIg density on normal and CLL PBM was unchanged after freezing. However, the fluorescence peak produced by frozen-thawed unlabeled cells was occasionally slightly broader than that of fresh cells, suggesting increased autofluorescence induced by freezing. These data indicate that frozen cell preparations may be utilized for the study of B-lymphocyte subsets as determined by sIg density. 相似文献
905.
906.
B. G. Wise D. Abbott C. J. Kelleher J. Haken G. Burton L. D. Cardozo 《BMJ (Clinical research ed.)》1992,304(6819):119-120
907.
P. B. Zikmanis R. V. Kruce L. P. Auzina M. V. Margevica M. J. Beker 《Applied microbiology and biotechnology》1988,27(5-6):507-509
Summary In comparison with intact yeast, dehydrated-rehydrated cells of Saccharomyces cerevisiae show significantly higher ethanol production from exogenous substrate under both anaerobic and aerobic conditions, particularly when low concentration (0.1%) of glucose are used. For populations with a higher percentage of viable rehydrated cells (above 70%) a more notable decrease in the Pasteur effect (the difference between the quantity of ethanol formed under anaerobic and aerobic conditions) is observed. 相似文献
908.
We screened a total of 460 stool samples from humans with and without underlying disease for the black yeast and neurotrope,
Exophiala dermatitidis using conventional and molecular identification procedures. Sixteen (3.5%) samples were positive. Twelve out of the sixteen positive samples where the fungus was recovered were from
individuals who had diarrhoea at the moment of isolation. Two asymptomatic positive cases were within the age bracket of 21–30.
Susceptibility testing of the strains using the CLSI M38 protocol suggests that almost all the strains were susceptible to
amphotericin B, 5 fluorocytosine, itraconazole, fluconazole and voriconazole. Considering the health risk currently posed
by this organism in Asia, especially among healthy younger subjects of the population and the scarcity of data on this fungus
in Africa in general and Nigeria in particular, some recommendations were suggested. 相似文献
909.
910.
Summary The rhizosphere microflora of arecanut palm under continuous application of organic manures and inorganic fertilizers was
studied. The nutrients applied are 100 g N, 40 g P2O5 and 140 g K2O/palm/year in the form of organics and inorganics. The application of organic manure increased the microbial population.
The increase in microbial population was observed between the rhizosphere samples collected at 0–30cm and 30–60 cm depths.
The surface cultivation of soil increased the microbial population.Trichoderma sp. andAspergillus sp. dominated in therhizosphere of arecanut palm.
Contribution No. 208. Central Plantation Crops Research Institute, Vittal-574243, Karnataka, India. 相似文献