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961.
G N Kryzhanovski? N G Lutsenko V N Grafova E I Danilova V K Lutsenko 《Biulleten' eksperimental'no? biologii i meditsiny》1986,101(4):399-401
The ability of serotonin derivatives to stimulate cAMP accumulation in isolated nerve terminals and lumbar enlargement of the spinal cord of normal rats was compared. The effect of the compounds on the intensity of spinal pain syndrome was also assessed. It has been established that substitutes injected into NH2-group of serotonin in 5-OH position attenuate the ability to stimulate cAMP accumulation in synaptosomes, with the effect more pronounced with substitutes of larger volume. A certain correlation between the ability of serotonin derivatives to stimulate adenylate cyclase in vivo and in vitro, on the one hand, and their analgetic effect, on the other hand, is suggested. 相似文献
962.
It was demonstrated that the nuclear matrix of rat liver possesses the protease activity. The specific activity of nuclear matrix proteases exceeds that of intact nuclei 7-fold. The optimum activity of nuclear matrix proteases is observed at pH 8-9. The protease activity of the nuclear matrix is inhibited by p-chloromercuribenzoate, N-ethylmaleimide, EDTA, phenylmethylsulfonyl fluoride. This suggests that thiol, serine and metalloproteases are associated with the nuclear matrix. 相似文献
963.
T. S. Serchenya A. G. Pryadko O. V. Sviridov 《Applied Biochemistry and Microbiology》2009,45(3):326-333
Four monoclonal antibodies (mAbs), G6, F9, H8, and B2, against human alpha-1-microglobulin (A1M) have been produced and characterized. The parameters of affinity (Kp ~ 109 M?1), epitope specificity (the additively binding G6/F9, G6/H8, G6/B2, F9/H8, and F9/B2 pairs), and the observed effect of reversibility of structural changes induced by chemical agents allow use of these mAbs in biospecific methods of A1M purification and quantitative determination. The application of mAbs to an A1M enzyme immunoassay (analytical sensitivity—0.5 μg/l) and one step isolation of pure A1M by immunoaffinity chromatography was described. 相似文献
964.
965.
966.
G V Kolusheva 《Arkhiv anatomii, gistologii i émbriologii》1987,93(7):10-15
The electron microscopic method has revealed early maturation of the protein-synthesizing apparatus of some cells, on the one hand, and underdevelopment of neuroblast volumes, appearance of artificial contacts, axonal degeneration and destruction in large trunks, on the other hand. Compensatory-restorative processes in the cell on the 14th and 21st days of the postnatal development result in a complete restoration of morphological organization of neurons and interneuronal connections in the hypothalamus of offspring of the morphine-dependent rats. 相似文献
967.
M R Kopantseva M Z Liudvig I I Uspenski? N A Tamarina V A Tsatrian L I Korochkin 《Zhurnal obshche? biologii》1990,51(1):125-140
A comparative electrophoretic study or ejaculatory bulb proteins in 29 different Drosophila species has been carried out. In all analyzed species, ejaculatory bulb contains a major component (designated as PEB). It has molecular mass of 61-65 kDa in the species of virilis group, 33-36 kDa in species of obscura group, and 34-56 kDa in species of melanogaster group. Using immunoblotting technique, we have demonstrated that PEB is introduced into organs of female sex tract during mating. The nature and significance of revealed interspecific differences in PEB proteins has been discussed. 相似文献
968.
969.
When the roots of Vicia faba L. beans were subjected to hypoxic stress, the activity of H+-ATPase on the peribacteroid membrane, as well as the transport of dicarboxylates (malate and succinate) mediated by this
enzyme, decreased. Since malate and succinate are the main carbon-containing metabolites involved in the energy supply to
bacteroids, this caused a change of the relation type from mutualism to commensalism, and the domination of the eukaryotes
over the prokaryotes consequently increased. 相似文献
970.
A M Belles T J Montville B P Wasserman 《Journal of industrial microbiology & biotechnology》2000,24(1):71-74
Alcohol-extractable, hydrophobic zein proteins contaminate starch granule surfaces and can be removed by enzymatic digestion
with thermolysin. The goal of this research was to find practical alternatives to thermolysin that might be used during the
corn wet-milling process. All of the commercial thermostable alkaline proteases studied (SP 709, Neutrase, and Spezyme FAN)
removed the zein proteins from various types of cornstarch, as demonstrated by the lack of protein bands below 30 kDa under
the reducing conditions of SDS-PAGE gel. Each enzyme removed the zein proteins as effectively as thermolysin removed them.
However, the removal of the zein protein did not reduce the quantity of free fatty acids associated with the starch. Journal of Industrial Microbiology & Biotechnology (2000) 24, 71–74.
Received 27 May 1999/ Accepted in revised form 01 October 1999 相似文献