全文获取类型
收费全文 | 318542篇 |
免费 | 37318篇 |
国内免费 | 134篇 |
出版年
2018年 | 2748篇 |
2016年 | 3741篇 |
2015年 | 5067篇 |
2014年 | 5994篇 |
2013年 | 8631篇 |
2012年 | 9575篇 |
2011年 | 9746篇 |
2010年 | 6597篇 |
2009年 | 5893篇 |
2008年 | 8535篇 |
2007年 | 8962篇 |
2006年 | 8371篇 |
2005年 | 8078篇 |
2004年 | 8165篇 |
2003年 | 7902篇 |
2002年 | 7801篇 |
2001年 | 14292篇 |
2000年 | 14321篇 |
1999年 | 11475篇 |
1998年 | 4020篇 |
1997年 | 4129篇 |
1996年 | 3928篇 |
1995年 | 3799篇 |
1994年 | 3800篇 |
1993年 | 3689篇 |
1992年 | 9805篇 |
1991年 | 9386篇 |
1990年 | 9211篇 |
1989年 | 8940篇 |
1988年 | 8347篇 |
1987年 | 7986篇 |
1986年 | 7211篇 |
1985年 | 7319篇 |
1984年 | 6043篇 |
1983年 | 5301篇 |
1982年 | 4173篇 |
1981年 | 3691篇 |
1980年 | 3511篇 |
1979年 | 6094篇 |
1978年 | 4547篇 |
1977年 | 4272篇 |
1976年 | 3968篇 |
1975年 | 4295篇 |
1974年 | 4651篇 |
1973年 | 4715篇 |
1972年 | 4355篇 |
1971年 | 4065篇 |
1970年 | 3435篇 |
1969年 | 3334篇 |
1968年 | 2962篇 |
排序方式: 共有10000条查询结果,搜索用时 93 毫秒
801.
Introduction into the structure of the linear hexapeptide DSLET (Tyr-D-Ser-Gly-Phe-Leu-Thr) or DTLET (Tyr-D-Thr-Gly-Phe-Leu-Thr) of tert-butyl groups as constraints different from cyclization leads to a large increase in the selectivity for delta opioid binding site in the case of DSTBULET [Tyr-D-Ser-(OtBu)-Gly-Phe-Leu-Thr] (Ki delta = 6.14 nM; Ki mu = 374 nM) and BUBU [Tyr-D-Ser(OtBu)-Gly-Phe-Leu-Thr(OtBu)] (Ki delta = 4.68 nM; Ki mu = 475 nM) or a loss of affinity for DTTBULET [Tyr-D-Thr(OtBu)-Gly-Phe-Leu-Thr] (Ki delta = 866 nM; Ki mu = 4500 nM). This puzzling behavior is studied here by 400-MHz 1H NMR spectroscopy in DMSO-d6 solution and by theoretical calculations. When DSLET and DTLET are compared, the reduction in energetically accessible phi and psi angles induced by the tert-butyl group in the D-Ser2 residue decreases the degree of freedom in the N-terminal part of the peptides. For DSTBULET and BUBU, the rigidification of the backbone evidenced by the appearance of the large NOE's of Phe4 NH-Gly3 alpha and Gly3 NH-alpha and by the loss of the C7 folding around the D-Ser2 residue found in DSLET could explain the drastic loss of affinity for mu opioid receptors. In DTTBULET, a large change in the spatial orientation around the D-Thr2 (OtBu) residue forces the aromatic rings far from each other.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献
802.
803.
804.
Nicotine and nicotine salts are taken up by the roots of plants from solutions, and when 0.01–0.001 % nicotine is used the plants become toxic to Aphis fabae and to Pieris brassicae larvae and can be shown to contain nicotine. The results with Phaedon cochleariae adults and larvae are less satisfactory. No systemic action is observed when the nicotine is watered on to soil in which plants are growing and no nicotine can be detected in the plants. Apparently the nicotine is decomposed in the soil.
When applied several times to the upper surface of a bean leaf nicotine kills aphids on the underside. There is some evidence that nicotine can be translocated further through the plant following leaf applications, but the toxic action at any distance is very weak in the plants used in the present experiments and can only be produced by frequent applications of rather concentrated nicotine solutions. Leaf absorption and subsequent translocation has not been observed with nicotine salts.
The various organic bases, including some piperidine phosphonites and allied compounds tested, are of very little interest as contact or systemic insecticides against aphids. 相似文献
When applied several times to the upper surface of a bean leaf nicotine kills aphids on the underside. There is some evidence that nicotine can be translocated further through the plant following leaf applications, but the toxic action at any distance is very weak in the plants used in the present experiments and can only be produced by frequent applications of rather concentrated nicotine solutions. Leaf absorption and subsequent translocation has not been observed with nicotine salts.
The various organic bases, including some piperidine phosphonites and allied compounds tested, are of very little interest as contact or systemic insecticides against aphids. 相似文献
805.
806.
Yeast tRNA is a convenient substrate for the assay of the ribonucleolytic activity of human angiogenin. The optimal pH, [NaCl], and temperature for tRNA cleavage by angiogenin are approximately 6.8, 15-30 mM, and approximately 55 degrees C, respectively, as compared with approximately 8.0, 100-200 mM, and approximately 65 degrees C, respectively, for RNase A. Polyanions and metals both inhibit angiogenin and RNase A but to different extents. 相似文献
807.
808.
Monkeys are 'flat–footed' in comparison to humans, but they are still able to utilize elastic strain energy stores in their feet to reduce the metabolic energy cost of running. During contact with the ground, bending moments act on the foot to produce a 'reversed arch', storing strain energy which is returned in the subsequent elastic recoil. This energy–saving mechanism has been investigated in dynamic bending tests and is discussed in relation to the arched foot of humans. 相似文献
809.
The nisin resistance determinant and an origin of replication on pNP40, a plasmid of about 60 kilobases that is present in Streptococcus lactis subsp. diacetylactis DRC3, was cloned on a 7.6-kilobase EcoRI fragment. When self-ligated, this fragment existed as an independent replicon (pFM011) and contained a 2.6-kilobase EcoRI-XbaI fragment encoding nisin resistance. 相似文献
810.
Cultured cells from the anterior pituitary glands of adult rats were treated with the tripeptide aldehyde proteinase inhibitor, BOC-DPhe-Phe-Lys-H. The addition of this tripeptide aldehyde decreased the in vitro release of prolactin to 25% of the control value, while the release of growth hormone in the same cultures decreased to 33% of the control value. Prolactin immunostaining was stronger in semithin sections of proteinase-inhibitor-treated cultures than in control sections. After 2 h treatment with the inhibitor, prolactin- and growth hormone-containing secretory granules were numerous, and the number of crinophagic vacuoles had increased. In the presence of the inhibitor, the overall cytoarchitecture of parenchymal cells was well preserved, and the pathway of the uptake of cationic ferritin appeared to be unaffected. 相似文献