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The reproductive periodicity of the sparid, Acanthopagrus pacificus, over four temporal scales is described. Acanthopagrus pacificus had a short spawning season between June and September, and within this, a peak reproductive period from July to early September. During the peak period there were several spawning peaks corresponding to a lunar periodicity, with intense reproductive activity on new and full moons that peaked during the period of the full moon when the tidal range was greatest. At the smallest temporal scale, spawning occurred at night on ebb tides. Because this study draws on data collected in 1991 and 1995, it provides a useful baseline against which to judge future changes in reproductive periodicity. 相似文献
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Growth of a human leukemic T-cell line (CEM C7) in 10(-6) M dexamethasone results in inhibition of growth and rapid loss of cell viability after a delay of approximately 18 to 24 hours. Analysis of dexamethasone-treated cells by flow-microfluorometry showed that they were arrested in the G1 phase of the cell cycle. Loss of cell viability began at the same time as G1 accumulation was first detectable, and 20% of all cells were found to be blocked in G1 at this time suggesting that loss of viability and G1 arrest were coincident events. Half-maximal and maximal effects on both viability and G1 arrest after 48 hours in steroid were nearly identical with respect to steroid concentration and corresponded to half-maximal and full occupancy of glucocorticoid specific receptor by hormone, consistent with a glucocorticoid receptor mediated mechanism for both phenomena. Most non-viable cells were arrested in G1, and accumulation of cells in G1 was irreversible; removal of steroid in the presence of colcemid did not result in a decreased fraction of G1 cells. Furthermore, dexamethasone treatment did not protect cells against the effects of 33258 Hoechst-amplified killing of bromodeoxyuridine substituted cells exposed to light. These results show that dexamethasone arrests these leukemic cells in G1 and strongly suggest that dexamethasone-treated cells are killed upon entry into G1. 相似文献