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71.
Akoachere M Squires RC Nour AM Angelov L Brojatsch J Abel-Santos E 《The Journal of biological chemistry》2007,282(16):12112-12118
Germination of Bacillus anthracis spores into the vegetative form is an essential step in anthrax pathogenicity. This process can be triggered in vitro by the common germinants inosine and alanine. Kinetic analysis of B. anthracis spore germination revealed synergy and a sequential mechanism between inosine and alanine binding to their cognate receptors. Because inosine is a critical germinant in vitro, we screened inosine analogs for the ability to block in vitro germination of B. anthracis spores. Seven analogs efficiently blocked this process in vitro. This led to the identification of 6-thioguanosine, which also efficiently blocked spore germination in macrophages and prevented killing of these cells mediated by B. anthracis spores. 6-Thioguanosine shows potential as an anti-anthrax therapeutic agent. 相似文献
72.
Alireza Shoae-Hassani Peyman Keyhanvar Alexander Marcus Seifalian Seyed Abdolreza Mortazavi-Tabatabaei Narmin Ghaderi Khosro Issazadeh Nour Amirmozafari Javad Verdi 《PloS one》2013,8(11)
Using phages is a novel field of cancer therapy and phage nanobioparticles (NBPs) such as λ phage could be modified to deliver and express genetic cassettes into eukaryotic cells safely in contrast with animal viruses. Apoptin, a protein from chicken anemia virus (CAV) has the ability to specifically induce apoptosis only in carcinoma cells. We presented a safe method of breast tumor therapy via the apoptin expressing λ NBPs. Here, we constructed a λ ZAP-CMV-apoptin recombinant NBP and investigated the effectiveness of its apoptotic activity on BT-474, MDA-MB-361, SKBR-3, UACC-812 and ZR-75 cell lines that over-expressing her-2 marker. Apoptosis was evaluated via annexin-V fluorescent iso-thiocyanate/propidium iodide staining, flow-cytometric method and TUNEL assay. Transfection with NBPs carrying λ ZAP-CMV-apoptin significantly inhibited growth of all the breast carcinoma cell lines in vitro. Also nude mice model implanted BT-474 human breast tumor was successfully responded to the systemic and local injection of untargeted recombinant λ NBPs. The results presented here reveal important features of recombinant λ nanobioparticles to serve as safe delivery and expression platform for human cancer therapy. 相似文献
73.
Badary OA AbdEl-Gawad HM Taha RA Ali AA Hamada FM 《Journal of biochemical and molecular toxicology》2003,17(2):86-91
The effects of benzo[a]pyrene (B[a]P) on some drug-metabolizing and antioxidant systems in liver, lung, and stomach were investigated in normal and protein malnutrition (PM) rats. PM significantly inhibited tissue glutathione (GSH) content and increased hepatic lipid peroxidation. Cytochrome P450 isoform CYP1A1 was significantly increased in various tissues (42-73%). Also, lung glutathione S-transferase (GST) activity was significantly decreased (19%) in PM rats. On the other hand, B[a]P significantly induced tissue GSH of control and PM rats. Also, hepatic lipid peroxidation were significantly increased in control rats treated with B[a]P. Superoxide dismutase (SOD) activity was decreased by B[a]P treatment in PM rat stomach. B[a]P significantly induced both quinone reductase (QR) (in all tissues) and hepatic GST of control and PM rats. GST activity in PM rat liver was significantly higher than that of control rat liver after B[a]P treatment. Also, B[a]P induced hepatic CYP1A1 by 32-fold and 27-fold (P < or = 0.05) in control and PM rats, respectively. Stomach and hepatic UDP-glucuronosyltransferase activities were significantly decreased (34%) and increased (74%), respectively by B[a]P in PM rats. The results suggest that PM status has a modifying effect on the response of some antioxidant and metabolizing systems to a well-known carcinogen risk. 相似文献
74.
75.
Maria Pérez-Marcos Maria Isabel Arnaldos Elena López-Gallego Aurelio Luna Azza Khedre Maria Dolores García 《法国昆虫学会纪事》2018,54(4):367-380
Summary. Since the entomosarcosaprophagous community is affected by environmental variables, it needs to be studied under different environmental conditions. In addition, because most studies are usually conducted with biomodels rather than human corpses, it is necessary to verify whether or not the type of decomposing animal matter affects the decomposition process itself, as well as the related fauna. For this reason, a study was conducted on the sarcosaprophagous Diptera community in two different environments of the Region of Murcia (SE Spain), using piglet and chicken carcasses, and Schoenly traps as collecting devices. To analyse possible differences regarding faunal composition among samples, a one-way Permutational Multivariate Analysis of Variance (PERMANOVA) was applied. The results indicate significant differences concerning the bait. In this sense, Calliphoridae, Muscidae, Fanniidae and Sarcophagidae families are the main groups responsible for such differences. In terms of environment-related differences, the only species that contribute are those of the dominant families, Calliphoridae and Muscidae. 相似文献
76.
77.
Nour E 《Biometrical journal. Biometrische Zeitschrift》1984,26(8):931-940
"A recent model for heterogeneous mortality by Vaupel et al....is shown to be based on incorrect definitions. An alternative formulation is presented. The results indicate that current methods for computing the survivorship and life expectation functions underestimate the true values. A method is given for determining the possible magnitude of this underestimation. The method is illustrated by a numerical example using U.S. data." 相似文献
78.
Propolis, a resinous substance collected by Apis mellifera bees from various plant sources and mixed with secreted beeswax, is a multifunctional material used by bees in the construction, maintenance, and protection of their hives. The collected propolis sample, from High Egypt, was dark-green with olive-odor. The minimal inhibition concentration (MIC) of propolis-ethanolic-extract, against Aeromonas hydrophila, was 80 μg Propolis-ethanolic-extract and crude propolis (1%) were added to artificial basal diet with (30% crude protein) to evaluate their efficacy on the fish growth-performance, immunostimulation and resistance to A. hydrophila. Two hundred and twenty-five Oreochromis niloticus (8 ± 0.45 g/fish) were divided into three equal treatments (T) of triplet replicates. The fish of T1 were fed on basal diet (control). The fish of T2 were given the basal diet, containing propolis-ethanolic-extract. The fish of T3 were given the basal diet containing crude propolis for 28 day. The fish were intraperitoneally challenged by A. hydrophila (0.2 × 107 cells ml−1) at the end of the feeding period and kept for 15 more days.The best growth rate and feed conversion ratio were obtained with T2. The increase in the average daily gain, specific growth rate and feed efficiency ratio were highly significances in T2 followed by T3 when compared with the control group. The HCT-level and monocyte-counts were increased (T2). No significant change, in the large lymphocytic-count was found among the three treatments (28–27–28%), while the neutrophil-count was significantly decreased (7%) with T2 and increased (13.11%) with the control. A significant increase in serum lysozyme and serum bactericidal activities was found with T2. The RLP against A. hydrophila was high with T2 and T3.The propolis-ethanolic-extract enhanced the growth, immunity and resistance of O. niloticus against A. hydrophila more than the crude propolis. 相似文献
79.
Van Eyken E Van Camp G Hendrickx JJ Demeester K Vandevelde A Azza JB Van de Heyning P Van Laer L 《Genetic testing》2007,11(3):231-234
GJB2 (Gap Junction protein beta type 2; Connexin 26, CX26) is known for its contribution to nonsyndromic recessive deafness (NSRD). One particular mutation, 35delG, a deletion of one guanine from a stretch of six leading to a frame shift early in the gene, has a high prevalence in populations from European descent. 35delG testing therefore has become a standard test in genetic diagnostic laboratories. Most of the currently available methods for the detection of 35delG are relatively time consuming, and not suited for high-throughput diagnostic testing. Within this paper we present a real-time PCR genotyping assay based on melting curve analysis, requiring only a single preparation step before the actual analysis. The assay was optimized on a panel of 48 samples with known 35delG genotypes and subsequently tested using a large Belgian population (N = 460) with unknown 35delG status. For the latter set of samples, real-time PCR results were validated with SNAPShot, an assay used in our laboratory for diagnostic purposes. The real-time PCR genotyping method has proven to be highly reliable, rapid, cost-effective, and suitable for high-throughput screening. We believe that this genetic test for 35delG will find widespread applications in the DNA diagnostic field. 相似文献
80.
Mohamed Eslam A. R. Abdel-Rahman Islam M. Zaki Magdi E. A. Al-Khdhairawi Ahmad Abdelhamid Mahmoud M. Alqaisi Ahmad M. Rahim Lyana binti Abd Abu-Hussein Bilal El-Sheikh Azza A. K. Abdelwahab Sayed F. Hassan Heba Ali 《Journal of molecular modeling》2023,29(3):1-1
Journal of Molecular Modeling - 相似文献