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121.
Ornatowska M Azim AC Wang X Christman JW Xiao L Joo M Sadikot RT 《American journal of physiology. Lung cellular and molecular physiology》2007,293(6):L1377-L1384
Triggering receptor expressed on myeloid cells 1 (TREM-1) is a recently discovered molecule that is expressed on the cell surface of monocytes and neutrophils. Engagement of TREM-1 triggers synthesis of proinflammatory cytokines in response to microbes, but the extent and mechanism by which TREM-1 modulates the inflammatory response is poorly defined. In the present study, we investigated the functional effects of blocking TREM-1 on the Toll-like receptor (TLR)4-mediated signaling pathway in macrophages. By transfecting cells with small hairpin interfering RNA molecules to TREM-1 (shRNA), we confirmed that TREM-1 mRNA and protein expression was greatly attenuated in RAW cells in response to treatment with LPS. PCR array for genes related to or activated by the TLR pathway revealed that although the expression of TLR4 itself was not significantly altered by silencing of TREM-1, expression of several genes, including MyD88, CD14, IkappaBalpha, IL-1beta, MCP-1, and IL-10 was significantly attenuated in the TREM-1 knockdown cells in response to treatment with LPS. These data indicate that expression of TREM-1 modulates the TLR signaling in macrophages by altering the expression of both adaptor and effector proteins that are critical to the endotoxin response. 相似文献
122.
Syed Ishtiaq Anjum Abdul Haleem Shah Muhammad Aurongzeb Junaid Kori M. Kamran Azim Mohammad Javed Ansari Li Bin 《Saudi Journal of Biological Sciences》2018,25(2):388-392
Gut microbiota has been recognized to play a beneficial role in honey bees (Apis mellifera). Present study was designed to characterize the gut bacterial flora of honey bees in north-west Pakistan. Total 150 aerobic and facultative anaerobic bacteria from guts of 45 worker bees were characterized using biochemical assays and 16S rDNA sequencing followed by bioinformatics analysis. The gut isolates were classified into three bacterial phyla of Firmicutes (60%), Proteobacteria (26%) and Actinobacteria (14%). Most of the isolates belonged to genera and families of Staphylococcus, Bacillus, Enterococcus, Ochrobactrum, Sphingomonas, Ralstonia, Enterobacteriaceae, Corynebacterium and Micrococcineae. Many of these bacteria were tolerant to acidic environments and fermented sugars, hence considered beneficial gut inhabitants and involved the maintenance of a healthy microbiota. However, several opportunistic commensals that proliferate in the hive environment including members Staphylococcus haemolyticus group and Sphingomonas paucimobilis were also identified. This is the first report on bee gut microbiota from north-west Pakistan geographically situated at the crossroads of Indian subcontinent and central Asia. 相似文献
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A rapid and efficient method for microdissection of the mouse egg is described. The dissection is carried out in hanging drops of medium surrounded by heavy liquid paraffin oil at room temperature. Eggs are first deformed into a cylindrical shape and then dissected at a predetermined site with a glass needle on a Leitz micromanipulator. The survival rate of the dissected fragments is 75–90% and between 20 and 30 eggs can be dissected in an hour. Development of the dissected eggs is at least as good as that described after other types of manipulation. Cytoplasts and karyoplasts of various sizes can be prepared, as well as gynogenetic and androgenetic eggs with different amounts of cytoplasm. This procedure may help to examine nuclear-cytoplasmic interactions in eggs reconstituted from a variety of fragments. 相似文献
127.
Hamide Hatamihanza Seyed Ebrahim Alavi Hasan Ebrahimi Shahmabadi Azim Akbarzadeh 《International journal of peptide research and therapeutics》2020,26(2):745-753
Tumor necrosis factor receptor 1 (TNFR1) is expressed on tumor cells and induces cell apoptosis through triggering proapoptotic cascade in these cells. Ago 相似文献
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Alireza Nakhaee Mohammad Bokaeian Azim Akbarzadeh Mohammad Hashemi 《Biological trace element research》2010,136(2):221-231
High blood glucose concentration in diabetes induces free radical production and, thus, causes oxidative stress. Damage of
cellular structures by free radicals play an important role in development of diabetic complications. In this study, we evaluated
effects of sodium tungstate on enzymatic and nonenzymatic markers of oxidative stress in brain of streptozotocin (STZ)-induced
diabetic rats. Rats were divided into four groups (ten rats in each group): untreated control, sodium tungstate-treated control,
untreated diabetic, and sodium tungstate-treated diabetic. Diabetes was induced with an intraperitoneal STZ injection (65 mg/kg
body weight), and sodium tungstate with concentration of 2 g/L was added to drinking water of treated animals for 4 weeks.
Diabetes caused a significant increase in the brain thiobarbituric acid reactive substances (P < 0.01) and protein carbonyl levels (P < 0.01) and a decrease in ferric reducing antioxidant power (P < 0.01). Moreover, diabetic rats presented a reduction in brain glucose-6-phosphate dehydrogenase (21%), superoxide dismutase
(41%), glutathione peroxidase (19%), and glutathione reductase (36%) activities. Sodium tungstate reduced the hyperglycemia
and restored the diabetes-induced changes in all mentioned markers of oxidative stress. However, catalase activity was not
significantly affected by diabetes (P = 0.4), while sodium tungstate caused a significant increase in enzyme activity of treated animals (P < 0.05). Data of present study indicated that sodium tungstate can ameliorate brain oxidative stress in STZ-induced diabetic
rats, probably by reducing of the high glucose-induced oxidative stress and/or increasing of the antioxidant defense mechanisms. 相似文献
130.
Epiphyton might have distinctive influence on the morphology of substrate macrophyte. In this article, we evaluate the influence
of epiphyton on the morphological characteristics of their substrate submerged macrophyte, Potamogeton perfoliatus under two light intensities. The experiment was carried out for a period of 84 days in 12 glass aquaria under laboratory
conditions. It was based on a 2 × 2 factorial design with epiphyton status (present or absent) and light intensity (200 or
80 μE m−2 s−1). Both epiphyton and light intensity had significant effects on the morphology and biomass allocation of the experimental
plants. The average number of leaves, total length of newly recruited shoots and diameter of stems were greater in the epiphyton-free
control plants than in the epiphyton-colonized plants under low light conditions. The plants with epiphyton allocated more
biomass in their rhizomes and roots (% relative to total biomass basis) when compared to the control plants in both light
intensities. There were also significant epiphyton–light interactions. The control plants under low light intensity showed
higher internodal elongation in their main shoots when compared to the plants under high light intensity as an adaptation
mechanism. Whereas the plants with epiphyton did not show such an adaptation. The new shoots of the control plants under low
light intensity did not show any internodal elongation as observed in the main shoots. Furthermore, the length of the leaves
of main shoots was larger in control plants with epiphyton and high light intensity than in plants with epiphyton and low
light intensity, but such a variation was absent in the new shoots. We conclude that the long-term colonization by epiphyton
and their shading effects induced the observed morphological changes in plants. 相似文献