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21.
Nguyen Van Tho Le Thi Huyen Trang Yoshitaka Murakami Emiko Ogawa Yasushi Ryujin Rie Kanda Hiroaki Nakagawa Kenichi Goto Kentaro Fukunaga Yuichi Higami Ruriko Seto Taishi Nagao Tetsuya Oguma Masafumi Yamaguchi Le Thi Tuyet Lan Yasutaka Nakano 《PloS one》2014,9(5)
Background
It is time-consuming to obtain the square root of airway wall area of the hypothetical airway with an internal perimeter of 10 mm (√Aaw at Pi10), a comparable index of airway dimensions in chronic obstructive pulmonary disease (COPD), from all airways of the whole lungs using 3-dimensional computed tomography (CT) analysis. We hypothesized that √Aaw at Pi10 differs among the five lung lobes and √Aaw at Pi10 derived from one certain lung lobe has a high level of agreement with that derived from the whole lungs in smokers.Methods
Pulmonary function tests and chest volumetric CTs were performed in 157 male smokers (102 COPD, 55 non-COPD). All visible bronchial segments from the 3rd to 5th generations were segmented and measured using commercially available 3-dimensional CT analysis software. √Aaw at Pi10 of each lung lobe was estimated from all measurable bronchial segments of that lobe.Results
Using a mixed-effects model, √Aaw at Pi10 differed significantly among the five lung lobes (R2 = 0.78, P<0.0001). The Bland-Altman plots show that √Aaw at Pi10 derived from the right or left upper lobe had a high level of agreement with that derived from the whole lungs, while √Aaw at Pi10 derived from the right or left lower lobe did not.Conclusion
In male smokers, CT-derived airway wall area differs among the five lung lobes, and airway wall area derived from the right or left upper lobe is representative of the whole lungs. 相似文献22.
Furukawa S Nojima N Nozaka S Hirayama S Satoh A Ogihara H Morinaga Y 《Bioscience, biotechnology, and biochemistry》2012,76(2):326-330
Lactic acid bacteria (LAB) mutants deficient in inter-species co-aggregation with yeast were spontaneously derived from Lactobacillus plantarum ML11-11, a significant mixed-species biofilm former in static co-cultures with budding yeasts. These non-co-aggregative mutants also showed significant decreases in mixed-species biofilm formation. These results suggest the important role of co-aggregation between LAB and yeast in mixed-species biofilm formation. Cell surface proteins obtained by 5 M LiCl extraction from the wild-type cells and non-co-aggregative mutant cells were analyzed by SDS-PAGE. There was an obvious difference in protein profiles. The protein band at 30 kDa was present abundantly in the wild-type cell surface fraction but was significantly decreased in the mutant cells. This band assuredly corresponded to the LAB surface factors that contribute to co-aggregation with yeasts. 相似文献
23.
Oguma K Katayama H Mitani H Morita S Hirata T Ohgaki S 《Applied and environmental microbiology》2001,67(10):4630-4637
UV inactivation, photoreactivation, and dark repair of Escherichia coli and Cryptosporidium parvum were investigated with the endonuclease sensitive site (ESS) assay, which can determine UV-induced pyrimidine dimers in the genomic DNA of microorganisms. In a 99.9% inactivation of E. coli, high correlation was observed between the dose of UV irradiation and the number of pyrimidine dimers induced in the DNA of E. coli. The colony-forming ability of E. coli also correlated highly with the number of pyrimidine dimers in the DNA, indicating that the ESS assay is comparable to the method conventionally used to measure colony-forming ability. When E. coli were exposed to fluorescent light after a 99.9% inactivation by UV irradiation, UV-induced pyrimidine dimers in the DNA were continuously repaired and the colony-forming ability recovered gradually. When kept in darkness after the UV inactivation, however, E. coli showed neither repair of pyrimidine dimers nor recovery of colony-forming ability. When C. parvum were exposed to fluorescent light after UV inactivation, UV-induced pyrimidine dimers in the DNA were continuously repaired, while no recovery of animal infectivity was observed. When kept in darkness after UV inactivation, C. parvum also showed no recovery of infectivity in spite of the repair of pyrimidine dimers. It was suggested, therefore, that the infectivity of C. parvum would not recover either by photoreactivation or by dark repair even after the repair of pyrimidine dimers in the genomic DNA. 相似文献
24.
Shindo K Tachibana A Tanaka A Toba S Yuki E Ozaki T Kumano T Nishiyama M Misawa N Kuzuyama T 《Bioscience, biotechnology, and biochemistry》2011,75(3):505-510
We performed combinational bioconversion of substituted naphthalenes with PhnA1A2A3A4 (an aromatic dihydroxylating dioxygenase from marine bacterium Cycloclasticus sp. strain A5) and prenyltransferase NphB (geranyltransferase from Streptomyces sp. strain CL190) or SCO7190 (dimethylallyltransferase from Streptomyces coelicolor A3(2)) to produce prenyl naphthalen-ols. Using 2-methylnaphthalene, 1-methoxynaphthalene, and 1-ethoxynaphthalene as the starting substrates, 10 novel prenyl naphthalen-ols were produced by combinational bioconversion. These novel prenyl naphthalen-ols each showed potent antioxidative activity against a rat brain homogenate model. 2-(2,3-Dihydroxyphenyl)-5,7-dihydroxy-chromen-4-one (2',3'-dihydroxychrysin) generated with another aromatic dihydroxylating dioxygenase and subsequent dehydrogenase was also geranylated at the C-5'-carbon by the action of NphB. 相似文献
25.
Xueyun Hu Satoru Makita Silvia Schelbert Shinsuke Sano Masanori Ochiai Tohru Tsuchiya Shigeaki F. Hasegawa Stefan H?rtensteiner Ayumi Tanaka Ryouichi Tanaka 《Plant physiology》2015,167(3):660-670
Chlorophyllase (CLH) is a common plant enzyme that catalyzes the hydrolysis of chlorophyll to form chlorophyllide, a more hydrophilic derivative. For more than a century, the biological role of CLH has been controversial, although this enzyme has been often considered to catalyze chlorophyll catabolism during stress-induced chlorophyll breakdown. In this study, we found that the absence of CLH does not affect chlorophyll breakdown in intact leaf tissue in the absence or the presence of methyl-jasmonate, which is known to enhance stress-induced chlorophyll breakdown. Fractionation of cellular membranes shows that Arabidopsis (Arabidopsis thaliana) CLH is located in the endoplasmic reticulum and the tonoplast of intact plant cells. These results indicate that CLH is not involved in endogenous chlorophyll catabolism. Instead, we found that CLH promotes chlorophyllide formation upon disruption of leaf cells, or when it is artificially mistargeted to the chloroplast. These results indicate that CLH is responsible for chlorophyllide formation after the collapse of cells, which led us to hypothesize that chlorophyllide formation might be a process of defense against chewing herbivores. We found that Arabidopsis leaves with genetically enhanced CLH activity exhibit toxicity when fed to Spodoptera litura larvae, an insect herbivore. In addition, purified chlorophyllide partially suppresses the growth of the larvae. Taken together, these results support the presence of a unique binary defense system against insect herbivores involving chlorophyll and CLH. Potential mechanisms of chlorophyllide action for defense are discussed.Plants have evolved both constitutive and inducible defense mechanisms against herbivores. Constitutive mechanisms include structural defenses (e.g. spines and trichomes) and specific chemical compounds. Constitutive defense mechanisms provide immediate protection against herbivore attacks, although they represent an energy investment by the plant regardless of whether herbivory occurs or not (Mauricio, 1998; Bekaert et al., 2012). By contrast, inducible defense mechanisms do not require an up-front energy cost, although such mechanisms may not be as immediate as constitutive ones when herbivore feeding occurs (Windram et al., 2012). Accordingly, plants exhibit both constitutive and inducible defense mechanisms against herbivory to balance the speed and cost of response. In this regard, it is plausible that the recruitment of abundant primary metabolites for defensive purposes might represent a substantial benefit to plants, providing both a swift and economical defense function.Toxic chemical compounds form an essential part in both constitutive and inducible defense mechanisms. However, these compounds are potentially a double-edged sword for plants, in a sense that they might pose toxic effects for both plants and herbivores. Plants have evolved an intricate binary system that prevents autointoxication by their own chemical compounds. Specifically, a toxic substance is stored in its inactive form and is spatially isolated from specific activating enzymes. These enzymes activate the substance when cells are disrupted by chewing herbivores (Saunders and Conn, 1978; Thayer and Conn, 1981; Morant et al., 2008). One of the most extensively studied binary defense systems is the glucosinolate/myrosinase system, in which the glucosinolate substrate and their hydrolyzing enzyme, a thioglucosidase myrosinase, are compartmentalized. Upon tissue damage, both the substrate and the enzyme come into contact to produce unstable aglycones, and various toxic compounds are then spontaneously produced (Bones and Rossiter, 1996). Another well-known example of the binary system is comprised of cyanogenic glucosides and β-glucosidase (Vetter, 2000; Mithöfer and Boland, 2012). In this system, nontoxic cyanogenic glycoside compounds are stored in the vacuole, whereas, the related glycosidase is localized in the cytoplasm. Upon cell destruction by chewing herbivores, the cyanogenic glycosides are hydrolyzed by glycosidase to yield unstable cyanohydrin that is either spontaneously or enzymatically converted into toxic hydrogen cyanide and a ketone or an aldehyde. Because the binary defense system is efficient and effective, a use of ubiquitous compounds for such systems would provide further benefits for plants.Tetrapyrrole compounds, in particular heme and chlorophyll, are abundant in plant cells. Despite their significant roles in various biological processes including photosynthesis and respiration, many tetrapyrroles are highly toxic to plant and animal cells, if present in excess amounts (Kruse et al., 1995; Meskauskiene et al., 2001). Their photodynamic properties can cause the generation of reactive oxygen species upon illumination, resulting in cell injury or direct cell death. For example, Tapper et al. (1975) showed that a tetrapyrrole compound (pheophorbide a), which is readily converted from dietary chlorophyll through the loss of magnesium and phytol, reduces the growth and survival rates of young albino rats through its photodynamic property. More recently, Jonker et al. (2002) demonstrated that dietary-derived pheophorbide a causes severe damages on the skin of mutant mice that lack a transporter to excrete pheophorbide a from cells. These studies indicate that incorporation of an excessive amount of tetrapyrrole compounds can induce photosensitization in animals. Previous studies also showed that tetrapyrroles have illumination-independent deleterious effects on insects. For example, pheophorbide a affected the assimilation of the plant sterols to synthesize developmental hormones of insects by inhibiting the activity of a key enzyme, cholesterol acyltransferase (Song et al., 2002). Moreover, some tetrapyrroles, including pheophorbide a, have been suggested to induce illumination-independent cell death in plants as well by an unknown mechanism (Hirashima et al., 2009). It is proposed that organisms use the toxicity of tetrapyrroles for their defense systems. The larvae of tortoise beetle (Chelymorpha alternans) even utilize pheophorbide a as a powerful deterrent in the fecal shield to protect themselves from their predators (Vencl et al., 2009). Kariola et al. (2005) suggested that a chlorophyll derivative, chlorophyllide, is involved in the defense against fungi, based on their observations that down-regulation of a chlorophyll-hydrolyzing enzyme, chlorophyllase (CLH), results in increased susceptibility of Arabidopsis (Arabidopsis thaliana) plants to the necrotrophic fungus Alternaria brassicicola.In this study, we examined the possibility that plants use tetrapyrroles for defense against herbivores by analyzing CLH, a well-known hydrolase common in plants. Chlorophyll consists of a tetrapyrrolic macrocycle and a hydrophobic phytol side chain (Fig. 1). Phytol hydrolysis results in the formation of chlorophyllide (Fig. 1), a less hydrophobic chlorophyll derivative, which has photochemical properties similar to chlorophyll. Two different plant enzymes are known to catalyze the cleavage of phytol, pheophytinase (PPH) and CLH. PPH is a chloroplast-located enzyme that specifically catalyzes the removal of phytol from Mg-free chlorophyll catabolites (Schelbert et al., 2009). This enzyme was only recently discovered and has been shown to be responsible for chlorophyll degradation during leaf senescence. By contrast, CLH has a broader substrate specificity and removes the side chain from chlorophyll or other chlorophyll derivatives (McFeeters et al., 1971). CLH activity was first reported in leaf extracts in 1913 (Willstätter and Stoll, 1913), but despite a century of research, in vivo function and intracellular localization of this enzyme remained controversial. Some reports have indicated CLH to localize to chloroplasts (Azoulay Shemer et al., 2008; Azoulay-Shemer et al., 2011), while Schenk et al. (2007), by examining the intracellular localization of transiently expressed CLH-GFP fusions, proposed Arabidopsis CLH to localize outside the chloroplast. Schenk et al. (2007) also reported that the lack of CLH does not affect chlorophyll degradation during leaf senescence. However, it remains possible that CLH is specifically involved in chlorophyll degradation in response to stresses that activate jasmonate signaling, such as wounding or pathogen attack. This hypothesis is based on the observation that the expression of a CLH gene was highest when methyl-jasmonate (MeJA; a derivative of jasmonic acid) was applied to Arabidopsis plants (Tsuchiya et al., 1999).Open in a separate windowFigure 1.Early steps of proposed chlorophyll breakdown pathways. MCS, Magnesium-dechelating substance.Here, we report that CLH is not involved in endogenous chlorophyll breakdown even when leaf senescence was promoted by jasmonate signaling. CLH is shown to localize to the chlorophyll-free endoplasmic reticulum (ER) and the tonoplast of intact plant cells. We found that CLH promotes the conversion of chlorophyll into chlorophyllide when leaf cells are disrupted or when CLH is genetically mislocalized to chloroplasts. To examine the possibility that plants use chlorophyll and CLH to form a binary defense system against herbivores, a generalist herbivore, Spodoptera litura larvae, was employed to investigate the toxicity of Arabidopsis leaves with genetically enhanced CLH activity and purified chlorophyllide. The results support our hypothesis, indicating plants to deploy an abundant photosynthetic pigment for defense against herbivores, which would be economic and provide adaptation benefits to plants. A potential mechanism of chlorophyllide action as part of the plant defense system is discussed based on the examination of chlorophyllide binding to the insect gut. 相似文献
26.
This paper describes courtship of Drosophila quadrilineata of the immigrans group and reports a species-specific element, abdomen bending, that has not been described in Drosophila. The male bends his abdomen right and left as a display to the female. During courtship the male circles around the female, bends his abdomen, and taps her. The male licks the female ovipositor repeatedly, often after the females extrusion. Although the females ovipositor extrusion is thought to indicate rejection of a courting male in many species, the male of D. quadrilineata continues courtship. It is possible that the extrusion is a females mating signal to the courting male. The male of D. quadrilineata mounts the female in a rearward position and the genitalia of both sexes couple after mounting. This mounting position is shared by the species belonging to the immigrans group. We never observed behavior in which it appeared as if the male displayed the black stripes to the female. Males court one another and a courtship chain is frequently formed even in the absence of females. The males abdomen bending and the females extrusion followed by the males licking are discussed. 相似文献
27.
Kimura T Yoshida A Tabuchi Y Ikari A Takeguchi N Asano S 《Journal of biochemistry》2002,131(6):923-932
Stable cell lines expressing the gastric proton pump alpha- and/or beta-subunits were constructed. The cell line co-expressing the alpha- and beta-subunits showed inward Rb(+) transport, which was activated by Rb(+) in a concentration-dependent manner. In the alpha+beta-expressing cell line, rapid recovery of intracellular pH was also observed after acid load, indicating that this cell line transported protons outward. These ion transport activities were inhibited by a proton pump inhibitor, 2-methyl-8-(phenylmethoxy)imidazo[1,2-a]pyridine-3-acetonitrile (SCH 28080). In a membrane fraction of the alpha+beta-expressing cell line, K(+)-stimulated ATPase (K(+)-ATPase) activity and the acylphosphorylation of the alpha-subunit were observed, both of which were also inhibited by SCH 28080. The specific activity and properties of the K(+)-ATPase were comparable to those found in the native gastric proton pump. In the stable cell lines, the alpha-subunit was retained in the intracellular compartment and was unstable in the absence of the beta-subunit, but it was stabilized and reached the cell surface in the presence of the beta-subunit. On the other hand, the beta-subunit was stable and able to travel to the cell surface in the absence of the alpha-subunit. These cell lines are ideal for the structure-function study of ion transport by the gastric proton pump as well as for characterization of the cellular regulation of surface expression of the functional proton pump. 相似文献
28.
29.
Chung CP Oeser A Avalos I Gebretsadik T Shintani A Raggi P Sokka T Pincus T Stein CM 《Arthritis research & therapy》2006,8(6):R186-7
The prevalence of ischemic heart disease and atherosclerosis is increased in patients with rheumatoid arthritis (RA). In the general population, but not in patients with systemic lupus erythematosus, the Framingham risk score identifies patients at increased cardiovascular risk and helps determine the need for preventive interventions. We examined the hypothesis that the Framingham score is increased and associated with coronary-artery atherosclerosis in patients with RA. The Framingham score and the 10-year cardiovascular risk were compared among 155 patients with RA (89 with early disease, 66 with long-standing disease) and 85 control subjects. The presence of coronary-artery calcification was determined by electron-beam computed tomography. The Framingham score was compared in patients with RA and control subjects, and the association between the risk score and coronary-artery calcification was examined in patients. Patients with long-standing RA had a higher Framingham score (14 [11 to 18]) (median [interquartile range]) compared to patients with early RA (11 [8 to 14]) or control subjects (12 [7 to 14], P < 0.001). This remained significant after adjustment for age and gender (P = 0.015). Seventy-six patients with RA had coronary calcification; their Framingham risk score was higher (14 [12 to 17]) than that of 79 patients without calcification (10 [5 to 14]) (P < 0.001). Furthermore, a higher Framingham score was associated with a higher calcium score (odds ratio [OR] = 1.20, 95% confidence interval [CI] 1.12 to 1.29, P < 0.001), and the association remained significant after adjustment for age and gender (OR = 1.15, 95% CI 1.02 to 1.29, P = 0.03). In conclusion, a higher Framingham risk score is independently associated with the presence of coronary calcification in patients with RA. 相似文献
30.