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171.
Karen T. Oliva Mustafa Ayhan Gillian Barker Nicole L. Dellios Michael A. Quinn Gregory E. Rice 《Clinical proteomics》2007,3(1-4):22-29
Objective
The aim of this study was to evaluate a multiple immunoaffinity protein depletion (multiple affinity removal system, MARS) pre-treatment strategy with subsequent two-dimensional polyacrylamide gel electrophoresis (2D PAGE) and peptide mass finger printing analysis for the detection of ovarian cancer-associated plasma proteins.Materials and Methods
Following immunoaffinity depletion, total plasma protein content was reduced by 84.2?±?1.8% (mean?±?SE, n?=?32). The number of proteins detected in the control and ovarian cancer groups was 349 and 357, respectively. This represented an increase in spot detection of almost twofold when compared to 2D PAGE displays of untreated plasma (174 spots). Of the proteins displayed, post-depletion, 300 (control) and 302 (ovarian cancer, OC) were common within each group. PDQuest analysis indicated that 109 protein spots were statistically different between the two groups and, of these, 59 exhibited greater than or equal to twofold difference in spot density (Student’s t test, p?=?0.01). Thirty-nine of these proteins were successfully identified with reliable confidence.Results and Discussion
The data obtained in this study demonstrates that immunodepletion of plasma before 2D PAGE profiling have generated identifiable plasma proteins that are differentially expressed in the high-grade ovarian cancer sample set compared to controls. This approach, therefore, may be useful in identifying candidate biomarkers for inclusion in multi-marker tests for ovarian cancer that may exhibit greater sensitivity and specificity than those currently available. It was evident, however, from the predominant identification of host response proteins that immunodepletion did not generate sufficient levels of enrichment of lower abundance tumor-specific proteins to facilitate detection. 相似文献172.
In this study, efficacy of seven different commercial disinfectant preparations was investigated against characteristic bacteria of a poultry slaughterhouse in Ankara (Turkey) by using paper disc-agar diffusion method and surface effectiveness test. According to paper disc-agar diffusion method, some disinfectants had wide efficacy against the test bacteria. The disinfectant effectiveness generally increased by the increasing of disinfectant concentration. However, some disinfectants were ineffective even though at their highest concentrations. The most effective disinfectant was B which contains QAC as active agent.Staphylococcus spp.,Staphyloccus aureus andEscherichia coli were the most sensitive bacteria to the disinfectants. Some pathogenic isolates, especiallySalmonella spp. andCampylobacter spp., were the most resistant ones to many of the disinfectants tested. The results of paper disc-agar diffusion method indicated the importance of characteristic bacterial strains of food plants as the test bacteria for disinfectant efficacy tests. Conversely of paper disc-agar diffusion method, all disinfectants were effective against the isolates by surface effectiveness test depending on exposure time. The disinfectants, except A and F, produced at least 3 log unit reduction during 5 min of exposure. However, all disinfectants at their lowest concentrations were effective against all tested bacteria during 15 and 30 min by this test. 相似文献
173.
Gulcin Tezcan Berrin Tunca Ahmet Bekar Murat Yalcin Saliha Sahin Ferah Budak Gulsah Cecener Unal Egeli Cevdet Demir Gokcen Guvenc Gozde Yilmaz Leman Gizem Erkan Hulusi Malyer Mevlut Ozgur Taskapilioglu Turkkan Evrensel Ayhan Bilir 《Cellular and molecular neurobiology》2015,35(2):175-187
Glioblastoma multiforme (GBM) is one of the deadliest human malignancies. A cure for GBM remains elusive, and the overall survival time is less than 1 year. Thus, the development of more efficient therapeutic approaches for the treatment of these patients is required. Induction of tumor cell death by certain phytochemicals derived from medicinal herbs and dietary plants has become a new frontier for cancer therapy research. Although the cancer suppressive effect of Ficus carica (fig) latex (FCL) has been determined in a few cancer types, the effect of this latex on GBM tumors has not been investigated. Therefore, in the current study, the anti-proliferative activity of FCL and the effect of the FCL–temozolomide (TMZ) combination were tested in the T98G, U-138 MG, and U-87 MG GBM cell lines using the WST-1 assay. The mechanism of cell death was analyzed using Annexin-V/FITC and TUNEL assays, and the effect of FCL on invasion was tested using the chick chorioallantoic membrane assay. To determine the effect of FCL on GBM progression, the expression levels of 40 GBM associated miRNAs were analyzed in T98G cells using RT-qPCR. According to the obtained data, FCL causes cell death in GBM cells with different responses to TMZ, and this effect is synergistically increased in combination with TMZ. In addition, the current study is the first to demonstrate the effect of FCL on modulation of let-7d expression, which may be an important underlying mechanism of the anti-invasive effect of this extract. 相似文献
174.
Ayhan Ulubelen Mahmut Miski Candan Johansson Esther Lee Tom J. Mabry Stephen A. Matlin 《Phytochemistry》1985,24(6):1386-1387
Six known terpenoids: vergatic acid, ursolic acid, crataegolic acid, lupane-3β-,11α, 20-triol, sclareol and sitosteryl 3β-glucoside were isolated from the leaves of Salvia palaestina and were identified by spectral data. Among the compounds, sclareol showed high activity against Staphylococcus aureus, S. epidermis, Escherichia coli, Proteus vulgaris and Pseudomonas aeruginosa, while the triterpenoids were not tested due to solubility problems. 相似文献
175.
In addition to two known furanogermacranes, a new furanogermacrane and a new and highly unstable furanoeremophilane were obtained from the fruits of Smyrnium cordifolium. The latter compound could be the precursor of a number of eremophilanolides obtained from some other Smyrnium species. 相似文献
176.
In order to assess the relationship between the oppiid mite species (Acari, Oribatida) collected from Turkey, traditional
and geometric morphometric methods were used. We have taken photographs of 128 specimens belonging to 11 species from six
subfamilies of Oppiidae. These photographs were digitized and 17 interval measurements were taken for traditional morphometric
analysis, and 20 landmarks were recognized on the ventral surface of each specimen for geometric morphometric analysis. The
effectiveness to separate taxa was compared between the two morphometric analyses. In both dendrograms Oppia nitens formed a single cluster, Oxyoppia ilicaensis was singled out from the remaining nine species, which were clustered together. Two species, Lauroppia fallax and Rhinoppia obsoleta, clustered together implying a very close relationship. 相似文献
177.
Ouvrier A Alves G Damon-Soubeyrand C Marceau G Cadet R Janny L Brugnon F Kocer A Pommier A Lobaccaro JM Drevet JR Saez F 《PloS one》2011,6(11):e26966
This work shows that an overload of dietary cholesterol causes complete infertility in dyslipidemic male mice (the Liver X Receptor-deficient mouse model). Infertility resulted from post-testicular defects affecting the fertilizing potential of spermatozoa. Spermatozoa of cholesterol-fed lxr-/- animals were found to be dramatically less viable and motile, and highly susceptible to undergo a premature acrosome reaction. We also provide evidence, that this lipid-induced infertility is associated with the accelerated appearance of a highly regionalized epididymal phenotype in segments 1 and 2 of the caput epididymidis that was otherwise only observed in aged LXR-deficient males. The epididymal epithelial phenotype is characterized by peritubular accumulation of cholesteryl ester lipid droplets in smooth muscle cells lining the epididymal duct, leading to their transdifferentiation into foam cells that eventually migrate through the duct wall, a situation that resembles the inflammatory atherosclerotic process. These findings establish the high level of susceptibility of epididymal sperm maturation to dietary cholesterol overload and could partly explain reproductive failures encountered by young dyslipidemic men as well as ageing males wishing to reproduce. 相似文献
178.
Two 14-day-old seedlings of maize (Zea mays L.) cultivars (3223 and Vero) were exposed to different concentrations of lead [0, 2, 5 and 8 mM Pb(NO3)2·4H2O] for 8 days. Exposure of maize cultivars to excess Pb resulted in a significant root growth inhibition though shoot growth
and absolute water content remained less affected. The results of chlorophyll a fluorescence indicated that the highly toxic Pb level affected photochemical efficiency in 3223, while no significant effect
was observed in the Vero. At the highly toxic Pb concentration, higher membrane leakage was observed in 3223 leaves than that
of Vero. This result was related to the accumulation of Pb. On the other hand, the results suggested that there were similar
responses in total soluble POD and GR activities with increasing Pb concentrations between both cultivars. But APX activity
significantly decreased at highly toxic Pb level in the Vero while a significant increase observed in the 3223. However, SOD
activity in 3223 significantly decreased at the highly toxic Pb concentration compared with that at 2 mM Pb concentration.
The results of the present study indicated that, Vero withstands excess Pb with its higher Pb accumulation capacity in roots
and better upregulated protective mechanisms compared to 3223. Therefore, Vero is more tolerant to Pb toxicity compared to
3223 which was found to be a less tolerant cultivar. 相似文献
179.
Epididymis response partly compensates for spermatozoa oxidative defects in snGPx4 and GPx5 double mutant mice 总被引:2,自引:0,他引:2
Noblanc A Peltier M Damon-Soubeyrand C Kerchkove N Chabory E Vernet P Saez F Cadet R Janny L Pons-Rejraji H Conrad M Drevet JR Kocer A 《PloS one》2012,7(6):e38565
We report here that spermatozoa of mice lacking both the sperm nucleus glutathione peroxidase 4 (snGPx4) and the epididymal glutathione peroxidase 5 (GPx5) activities display sperm nucleus structural abnormalities including delayed and defective nuclear compaction, nuclear instability and DNA damage. We show that to counteract the GPx activity losses, the epididymis of the double KO animals mounted an antioxydant response resulting in a strong increase in the global H(2)O(2)-scavenger activity especially in the cauda epididymis. Quantitative RT-PCR data show that together with the up-regulation of epididymal scavengers (of the thioredoxin/peroxiredoxin system as well as glutathione-S-transferases) the epididymis of double mutant animals increased the expression of several disulfide isomerases in an attempt to recover normal disulfide-bridging activity. Despite these compensatory mechanisms cauda-stored spermatozoa of double mutant animals show high levels of DNA oxidation, increased fragmentation and greater susceptibility to nuclear decondensation. Nevertheless, the enzymatic epididymal salvage response is sufficient to maintain full fertility of double KO males whatever their age, crossed with young WT female mice. 相似文献
180.
Harman E Karadeniz M Avci CB Zengi A Erdogan M Yalcin M Cetinkalp S Ahmet Gokhan O Gunduz C Saygili F Yilmaz C 《Gynecological endocrinology : the official journal of the International Society of Gynecological Endocrinology》2012,28(10):830-833
Objective: Infertility and reproductive impairment can be compromised by abnormalities in both endocrine and immune system. TNF-α promotes apoptotic cell death in fetal membrane tissues and pro-inflammatory, proapoptotic, and procoagulant properties of TNF-α probably contribute to widely accepted abortogenic profile of this cytokine. The aim of this study was to assess the alteration in the levels of TSH, FT3, FT4, TNF-α, osteopontin in pregnant and controls. Methods: Study subjects were 28 pregnant women, 28 non-pregnant women, and 28 healthy controls. All subjects underwent venous blood drawing for levels of TNF-α, osteopontin, and also hormonal assays including the levels of anti-TPO, anti-TG antibodies, TSH, FT3, FT4. Results: Both patient and control groups are similar in terms of age. Pregnancy age in conceived patients is 23.64 ± 2.040. No statistically meaningful relation was found in correlation analysis between TNF-α and osteopontin among the groups (p = 0.963). Anti-thyroglobuline antibody and anti-microsomal antibody levels were found to be higher in patients with non-pregnant patients with Hashimoto thyroiditis than the control group (p < 0.001). No statistically meaningful relation was found in terms of TNF-α (p = 0.66) and osteopontin serum levels (p = 0.50) in patient groups with or without miscarriage history. Conclusions: In our study, no statistically meaningful relation was found in terms of TNF-α and osteopontin serum levels in patient groups with and without miscarriage history. 相似文献