全文获取类型
收费全文 | 347篇 |
免费 | 17篇 |
出版年
2023年 | 6篇 |
2022年 | 3篇 |
2021年 | 8篇 |
2020年 | 7篇 |
2019年 | 5篇 |
2018年 | 17篇 |
2017年 | 22篇 |
2016年 | 20篇 |
2015年 | 20篇 |
2014年 | 20篇 |
2013年 | 32篇 |
2012年 | 36篇 |
2011年 | 25篇 |
2010年 | 17篇 |
2009年 | 15篇 |
2008年 | 17篇 |
2007年 | 25篇 |
2006年 | 6篇 |
2005年 | 12篇 |
2004年 | 13篇 |
2003年 | 18篇 |
2002年 | 6篇 |
2001年 | 2篇 |
2000年 | 3篇 |
1999年 | 2篇 |
1998年 | 1篇 |
1997年 | 1篇 |
1995年 | 1篇 |
1993年 | 1篇 |
1977年 | 1篇 |
1971年 | 2篇 |
排序方式: 共有364条查询结果,搜索用时 15 毫秒
101.
Ayşegül Güzel-Ozantürk Rıza Köksal Özgül Özlem Ünal Burcu Hişmi Halil İbrahim Aydın Serap Sivri Ayşegül Tokatlı Turgay Coşkun Erol Aksöz Ali Dursun 《Gene》2013
Lysinuric protein intolerance is an autosomal recessive metabolic disorder caused by defective transport of the cationic amino acids lysine, arginine and ornithine in the epithelial cells of the basolateral membrane in the small intestine and renal tubules. Mutations in the solute carrier family 7, member 7, SLC7A7, gene cause this multisystemic disease with a variety of clinical symptoms such as hepatosplenomegaly, osteoporosis, hypotonia, developmental delay, pulmonary insufficiency or end-stage renal disease. In the present study, genomic structure of SLC7A7 in six Turkish patients with lysinuric protein intolerance was examined in order to detect disease causing mutations by denaturing high pressure liquid chromatography and direct sequencing. Four novel mutations were identified in SLC7A7: c.223insGTC, p.Val74_Ile75insVal; c.283insTGG, p.Glu94_Thr95insTrp; c.344_347delTTGC, p.Leu115LeufsX53; and c.1099insT, p.Ile367TyrfsX16. Clinical and biochemical findings were evaluated together with these molecular analyses. 相似文献
102.
Tanrikulu S Doğru-Abbasoğlu S Ozderya A Ademoğlu E Karadağ B Erbil Y Uysal M 《Cell biochemistry and function》2011,29(3):244-248
Oxidative DNA damage, caused by either endogenous or exogenous sources of reactive oxygen species (ROS), has been linked several diseases including Graves' disease (GD). 7,8‐Dihydro‐8‐oxoguanine (8‐oxoG) is a major lesion produced by ROS and is considered a key biomarker of oxidative DNA damage. In humans, 8‐oxoG is mainly repaired by 8‐oxoguanine DNA N‐glycosylase‐1 (hOGG1), which is an essential component of the base excision repair (BER) pathway. The functional studies showed that hOGG1 Ser326Cys polymorphism is associated with the reduced DNA repair activity and increased risk for some oxidative stress‐related diseases. In this study, we firstly investigated hOGG1 Ser326Cys polymorphism in GD. According to our results, Cys/Cys genotype frequency in the GD patients (23.4%) was significantly higher than the controls (9.2%). Cys/Cys genotype had an 3.5‐fold [95% CI (confidence interval): 2.10–6.01, p < 0.001] the Cys allele had 1.83‐fold (95% CI: 1.43–2.34, p < 0.001) increase in the risk for developing GD. Our results suggest that Ser326Cys polymorphism of the hOGG1 gene is associated with GD risk. Copyright © 2011 John Wiley & Sons, Ltd. 相似文献
103.
Lalle M Currà C Ciccarone F Pace T Cecchetti S Fantozzi L Ay B Breton CB Ponzi M 《The Journal of biological chemistry》2011,286(2):1227-1236
The malaria parasite invades the terminally differentiated erythrocytes, where it grows and multiplies surrounded by a parasitophorous vacuole. Plasmodium blood stages translocate newly synthesized proteins outside the parasitophorous vacuole and direct them to various erythrocyte compartments, including the cytoskeleton and the plasma membrane. Here, we show that the remodeling of the host cell directed by the parasite also includes the recruitment of dematin, an actin-binding protein of the erythrocyte membrane skeleton and its repositioning to the parasite. Internalized dematin was found associated with Plasmodium 14-3-3, which belongs to a family of conserved multitask molecules. We also show that, in vitro, the dematin-14-3-3 interaction is strictly dependent on phosphorylation of dematin at Ser(124) and Ser(333), belonging to two 14-3-3 putative binding motifs. This study is the first report showing that a component of the erythrocyte spectrin-based membrane skeleton is recruited by the malaria parasite following erythrocyte infection. 相似文献
104.
Değirmenci A Döşkaya M Caner A Ciçek C Korkmaz M Gürüz Y Uner A 《Experimental parasitology》2011,127(1):1-8
Nitric oxide (NO) and NO-derived reactive nitrogen species (RNS) are present in the food vacuole (FV) of Plasmodium falciparum trophozoites. The product of PFL1555w, a putative cytochrome b5, localizes in the FV membrane, similar to what was previously observed for the product of PF13_0353, a putative cytochrome b5 reductase. These two gene products may contribute to NO generation by denitrification chemistry from nitrate and/or nitrite present in the erythrocyte cytosol. The possible coordination of NO to heme species present in the food vacuole was probed by resonance Raman spectroscopy. The spectroscopic data revealed that in situ generated NO interacts with heme inside the intact FVs to form ferrous heme nitrosyl complexes that influence intra-vacuolar heme solubility. The formation of heme nitrosyl complexes within the FV is a previously unrecognized factor that could affect the equilibrium between soluble and crystallized heme within the FV in vivo. 相似文献
105.
Atmospheric pollen was collected with a Burkard sporetrap in Ankara, Turkey from January 1993 to January1994. The sum of the annual totals of the dailyconcentration of pollen, belonging to 44 taxa, was57,735. A relatively high pollen concentration wasrecorded in June which could be caused by higher windspeed and lower rainfall. Pinaceae,Cupressaceae/Taxaceae, Gramineae, Platanus, Populus,Moraceae, Chenopodiaceae/Amaranthaceae, Acer, Quercus,Betula, Salix, Rumex and Plantago are found tobe the dominant pollen types in the atmosphere inAnkara. 相似文献
106.
107.
Malay O Bayraktar O Batigün A 《International journal of biological macromolecules》2007,40(4):387-393
This study aimed to investigate the pH-induced complexation of silk fibroin (SF) and hyaluronic acid (HA). SF-HA complex coacervation was investigated by monitoring turbidity of the SF-HA system under slow acidification. Gravimetric analysis was performed to determine the yield of complex coacervation and viscosity of the system was measured to study the formation of the complexes at different pH values. The influences of total biopolymer concentration and biopolymer weight ratio on complex coacervation were examined during the analyses. Formation of the complexes was evidenced by the minimum viscosity and the maximum turbidity observed in the system. SF-HA complexes were formed within the pH-window of 2.5-3.5 regardless of the total biopolymer concentration or biopolymer ratio. Complex coacervation of SF-HA showed a reversible behavior and coacervation could be handled even in excess amounts of the biopolymers, which pointed out a non-stoichiometric complexation. 相似文献
108.
Eren I Naziroğlu M Demirdaş A Celik O Uğuz AC Altunbaşak A Ozmen I Uz E 《Neurochemical research》2007,32(3):497-505
Venlafaxine is an approved antidepressant that is an inhibitor of both serotonin and norepinephrine transporters. Medical
treatment with oral venlafaxine can be beneficial to depression due to reducing free radical production in the brain and medulla
of depression- induced rats because oxidative stress may a play role in some depression. We investigated the effect of venlafaxine
administration and experimental depression on lipid peroxidation and antioxidant levels in cortex brain, medulla and erythrocytes
of rats. Thirty male wistar rats were used and were randomly divided into three groups. Venlafaxine (20 mg/kg) was orally
supplemented to depression-induced rats constituting the first group for four week. Second group was depression-induced group
although third group was used as control. Depressions in the first and second groups were induced on day zero of the study
by chronic mild stress. Brain, medulla and erythrocytes samples were taken from all animals on day 28. Depression resulted
in significant decrease in the glutathione peroxidase (GSH-Px) activity and vitamin C concentrations of cortex brain, glutathione
(GSH) value of medulla although their levels were increased by venlafaxine administration to the animals of depression group.
The lipid peroxidation levels in the three tissues and nitric oxide value in cortex brain elevated although their levels were
decreased by venlafaxine administration. There were no significant changes in cortex brain vitamin A, erythrocytes vitamin
C, GSH-Px and GSH, medulla vitamin A, GSH and GSH-Px values. In conclusion, cortex brain within the three tissues was most
affected by oxidative stress although there was the beneficial effect of venlafaxine in the brain of depression-induced rats
on investigated antioxidant defenses in the rat model. The treatment of depression by venlafaxine may also play a role in
preventing oxidative stress.
Abstract of the paper was submitted in 1st Ion Channels and Oxidative Stress Congress, 14–16 September 2006, Isparta, Turkey. 相似文献
109.
Antimicrobial activity of extracts of the lichen Parmelia sulcata and its salazinic acid constituent
Candan M Yilmaz M Tay T Erdem M Türk AO 《Zeitschrift für Naturforschung. C, Journal of biosciences》2007,62(7-8):619-621
The antimicrobial activity of the acetone, chloroform, diethyl ether, methanol, and petroleum ether extracts of the lichen Parmelia sulcata and its salazinic acid constituent have been screened against twenty eight food-borne bacteria and fungi. All of the extracts with the exception of the petroleum ether extract showed antimicrobial activity against Aeromonas hydrophila, Bacillus cereus, Bacillus subtilis, Listeria monocytogenes, Proteus vulgaris, Yersinia enterocolitica, Staphylococcus aureus, Streptococcus faecalis, Candida albicans, Candida glabrata, Aspergillus niger, Aspergillus fumigatus, and Penicillium notatum. Salazinic acid did not show antimicrobial activity against L. monocytogenes, P. vulgaris, Y. enterocolitica, and S. faecalis but showed activity against Pseudomonas aeruginosa and Salmonella typhimurium as well. The MIC values of the extracts and the acid for the bacteria and fungi have also been determined. 相似文献
110.
Erdoğan Ayşegül Karataş Ayça Büşra Demirel Zeliha Dalay Meltem Conk 《Journal of applied phycology》2022,34(1):301-309
Journal of Applied Phycology - Microalgae with high growth rates represent a potential source of carotenoids including fucoxanthin. For commercial fucoxanthin production, choosing the cultivation... 相似文献