全文获取类型
收费全文 | 312篇 |
免费 | 26篇 |
出版年
2023年 | 3篇 |
2022年 | 3篇 |
2021年 | 4篇 |
2019年 | 5篇 |
2018年 | 3篇 |
2016年 | 6篇 |
2015年 | 15篇 |
2014年 | 15篇 |
2013年 | 11篇 |
2012年 | 19篇 |
2011年 | 20篇 |
2010年 | 10篇 |
2009年 | 9篇 |
2008年 | 11篇 |
2007年 | 15篇 |
2006年 | 11篇 |
2005年 | 8篇 |
2004年 | 5篇 |
2003年 | 14篇 |
2002年 | 5篇 |
2001年 | 3篇 |
2000年 | 3篇 |
1998年 | 7篇 |
1995年 | 3篇 |
1994年 | 3篇 |
1993年 | 5篇 |
1991年 | 7篇 |
1990年 | 5篇 |
1989年 | 8篇 |
1988年 | 7篇 |
1987年 | 5篇 |
1986年 | 9篇 |
1985年 | 3篇 |
1984年 | 4篇 |
1983年 | 6篇 |
1982年 | 4篇 |
1981年 | 3篇 |
1980年 | 3篇 |
1977年 | 8篇 |
1976年 | 4篇 |
1974年 | 3篇 |
1973年 | 4篇 |
1972年 | 3篇 |
1971年 | 2篇 |
1970年 | 3篇 |
1969年 | 2篇 |
1968年 | 2篇 |
1967年 | 3篇 |
1966年 | 5篇 |
1965年 | 3篇 |
排序方式: 共有338条查询结果,搜索用时 31 毫秒
71.
D. Aviv S. Bleichman P. Arzee-Gonen E. Galun 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1984,67(6):499-504
Summary Cybrid plants having the nuclear genomes of one species and either or both plastomes and chondriomes of another species were obtained by fusing protoplasts of Nicotiana sylvestris, as recipients, with X-irradiated protoplasts of N. rustica as donors of chloroplasts and mitochondria. Forty-nine flowering plants, derived from 28 calli, were analysed. As expected, they all had N. sylvestris (i.e. recipients) morphology. Chloroplast DNA restriction patterns indicated that 8 and 41 plants had N. rustica and N. sylvestris plastomes, respectively. Some of the plants with either type of plastomes produced sterile pollen but none showed anther malformation typical to alloplasmic male sterility. Chondriome identification by mitochondrial DNA restriction analysis of cybrid plants revealed only restriction patterns which were either similar or identical to those of N. sylvestris while no cybrids with N. rustica restriction patterns were detected. 相似文献
72.
Isolation of two cDNA clones from tomato containing two different superoxide dismutase sequences 总被引:6,自引:0,他引:6
Rafael Perl-Treves Benedetta Nacmias Dvora Aviv Elisha P. Zeelon Esra Galun 《Plant molecular biology》1988,11(5):609-623
A cDNA library was derived from the poly(A)+ RNA of young tomato leaves. The library was cloned in a gt11 system and screened by synthetic oligonucleotide probes having sequences that match the codes of conserved regions of amino acid sequences of Cu,Zn superoxide dismutase (SOD) proteins from a wide range of eukaryotic organisms. Two cDNAs were isolated, cloned and sequenced. One of the cDNAs, P31, had a full-size open reading frame of 456 bp with a deduced amino acid sequence having an 80% homology with the deduced amino acid sequence of the cytosolic SOD-2 cDNA of maize. The other cDNA, T10 (extended by T1), had a 651 bp open reading frame that revealed, upon computer translation, 90% homology to the amino acid sequence of mature spinach chloroplast SOD. The 5 end of the reading frame seems to code for a putative transit peptide. This work thus suggests for the first time an amino acid sequence for the transit peptide of chloroplast SOD. Northern hybridizations indicated that each of the P31 and T10 clones hybridized to a blotted poly(A)+ RNA species. These two species are differentially expressed in the plant organs: e.g., the species having the T10 sequence was detected in the leaves but not in roots, while the one with the P31 sequence was expressed in both leaves and roots. The cDNA clones P31 and T10 were also hybridized to Southern blots of endonuclease fragmented tomato DNA. The clones hybridized to specific fragments and no cross hybridization between the two clones was revealed under stringent hybridization conditions; the hybridization pattern indicated that, most probably, only one locus is coding for each of the two mRNA species. 相似文献
73.
74.
75.
76.
77.
78.
Paola Sebastiani Monty Montano Annibale Puca Nadia Solovieff Toshio Kojima Meng C. Wang Efthymia Melista Micah Meltzer Sylvia E. J. Fischer Stacy Andersen Stephen H. Hartley Amanda Sedgewick Yasumichi Arai Aviv Bergman Nir Barzilai Dellara F. Terry Alberto Riva Chiara Viviani Anselmi Alberto Malovini Aya Kitamoto Motoji Sawabe Tomio Arai Yasuyuki Gondo Martin H. Steinberg Nobuyoshi Hirose Gil Atzmon Gary Ruvkun Clinton T. Baldwin Thomas T. Perls 《PloS one》2009,4(12)
Background
The strong familiality of living to extreme ages suggests that human longevity is genetically regulated. The majority of genes found thus far to be associated with longevity primarily function in lipoprotein metabolism and insulin/IGF-1 signaling. There are likely many more genetic modifiers of human longevity that remain to be discovered.Methodology/Principal Findings
Here, we first show that 18 single nucleotide polymorphisms (SNPs) in the RNA editing genes ADARB1 and ADARB2 are associated with extreme old age in a U.S. based study of centenarians, the New England Centenarian Study. We describe replications of these findings in three independently conducted centenarian studies with different genetic backgrounds (Italian, Ashkenazi Jewish and Japanese) that collectively support an association of ADARB1 and ADARB2 with longevity. Some SNPs in ADARB2 replicate consistently in the four populations and suggest a strong effect that is independent of the different genetic backgrounds and environments. To evaluate the functional association of these genes with lifespan, we demonstrate that inactivation of their orthologues adr-1 and adr-2 in C. elegans reduces median survival by 50%. We further demonstrate that inactivation of the argonaute gene, rde-1, a critical regulator of RNA interference, completely restores lifespan to normal levels in the context of adr-1 and adr-2 loss of function.Conclusions/Significance
Our results suggest that RNA editors may be an important regulator of aging in humans and that, when evaluated in C. elegans, this pathway may interact with the RNA interference machinery to regulate lifespan. 相似文献79.
The somatic hypermutation (SHM) of Immunoglobulin variable (V) regions is a key process in the generation of antibody diversity. The growing number of datasets of point mutations that occur during SHM in mice and humans often include comparisons between wild-type and individuals or strains genetically defective in the repair mechanisms that contribute to SHM. However, it has been difficult to compare the results of different studies because the analyses have not been standardized for criteria such as correction for base composition and the inclusion of unique mutations. If many mutations are involved, the analysis can also be time consuming. To overcome these problems and facilitate a standardized analysis and display of similar data, we present a webserver (SHMTool) for comparing SHM datasets, available at http://scb.aecom.yu.edu/shmtool. 相似文献
80.
We propose that rearrangements between short tandem repeated sequences occur by errors made during a replication fork repair pathway involving a replication template switch. We provide evidence here that the DnaK chaperone of E. coli controls this template switch repair process. Mutants in dnaK are sensitive to replication fork damage and exhibit high expression of the SOS response, indicative of repair deficiency. Deletion and expansion of tandem repeats that occur by replication misalignment ("slippage") are also DnaK dependent. Because mutations in dnaX encoding the gamma and tau subunits of DNA polymerase III mimic dnaK phenotypes and are genetically epistatic, we propose that the DnaKJ chaperone remodels the replisome to facilitate repair. The fork remains largely intact because PriA or PriC restart proteins are not required. We also suggest that the poorly defined RAD6-RAD18-RAD5 mechanism of postreplication repair in eukaryotes occurs by an analogous mechanism to the DnaK template-switch pathway in prokaryotes. 相似文献