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381.
382.
Rakeshkumar M. JainKalpana Mody Avinash MishraBhavanath Jha 《Carbohydrate polymers》2012,87(3):2320-2326
Production of biosurfactant from an alkaliphilic bacterium Cronobacter sakazakii (accession no. JN398668) was screened by haemolytic assay, emulsifying activity and surface tension measurement. Biosurfactant, comprised of total sugars (73.3%), reducing sugars (1.464%), protein (11.9%), uronic acid (15.98%) and sulfate (6.015%), showed low viscosity with pseudoplastic rheological behavior and exhibited significant emulsification activity with oils and hydrocarbons. A series of low and mid range mass peaks (m/z) corresponding to mono-, di-, tri- and oligosaccharides were detected in the positive ion reflector mode of MALDI TOF-TOF MS. GC-MS analysis revealed composition of monosaccharide moieties (w/w) viz. glucose (14%), mannose (24%), galactose (14%), xylose (20%) and arabinose (1.9%). 1H NMR, FT-IR and EDX analyses confirmed the characteristic various functional groups, bonds and elements respectively. Thermostability (up to 260 °C) and CI (0.456) were determined by TG and DSC analyses. Inherent properties of biosurfactant make it a potential candidate for bioremediation of oil and hydrocarbons. 相似文献
383.
Glenn A. Van Buskirk Satish Asotra Christopher Balducci Prabir Basu Gerald DiDonato Angelica Dorantes W. Mark Eickhoff Tapash Ghosh Mario A. González Theresa Henry Matthew Howard Jason Kamm Steven Laurenz Ryan MacKenzie Richard Mannion Patrick K. Noonan Terrance Ocheltree Umesh Pai Richard P. Poska Michael L. Putnam Ramani R. Raghavan Colleen Ruegger Eric Sánchez Vinod P. Shah Zezhi Jesse Shao Russell Somma Vijay Tammara Avinash G. Thombre Bruce Thompson Robert J. Timko Satyam Upadrashta Sivakumar Vaithiyalingam 《AAPS PharmSciTech》2014,15(3):665-693
In this whitepaper, the Manufacturing Technical Committee of the Product Quality Research Institute provides information on the common, best practices in use today in the development of high-quality chemistry, manufacturing and controls documentation. Important topics reviewed include International Conference on Harmonization, in vitro–in vivo correlation considerations, quality-by-design approaches, process analytical technologies and current scale-up, and process control and validation practices. It is the hope and intent that this whitepaper will engender expanded dialog on this important subject by the pharmaceutical industry and its regulatory bodies. 相似文献
384.
Three caprolactam-degrading bacterial isolates grew in liquid synthetic medium containing solubilised solid waste of a nylon-6
production plant as the sole source of carbon and nitrogen. Typically, the caprolactam content of solid waste was decreased
by 95% in 72 h by Alcaligenes faecalis. A. faecalis was the most potent caprolactam-degrading bacterium out of the three isolates. The biomass of the bacteria obtained by growth
in the solubilised solid waste medium had the ability to decolourise some synthetic azo and triphenylmethane dyes. Decolourisation
of dyes was obtained in static condition, in synthetic medium which contained only the components of the solid waste as the
sole sources of carbon and nitrogen and also in nutritionally rich medium. The supplementation of yeast extract to solid waste
medium did not increase the efficiency of decolourisation in case of two of the bacterial cultures. Depending on the dye,
medium and bacteria used, decolourisation in the range of 35–94% was achieved in 48–96 h. The decolourisation was not due
to the adsorption of the dyes by the bacterial biomass except in case of Procion Blue MR and Black B. Based on these observations,
the simultaneous biological treatment of the solid waste of nylon-6 plant and the decolourisation of synthetic dyes present
in wastewater or solid waste is envisaged. 相似文献
385.
Background
Chlamydia pecorum is an obligate intracellular bacterium and the causative agent of reproductive and ocular disease in several animal hosts including koalas, sheep, cattle and goats. C. pecorum strains detected in koalas are genetically diverse, raising interesting questions about the origin and transmission of this species within koala hosts. While the ompA gene remains the most widely-used target in C. pecorum typing studies, it is generally recognised that surface protein encoding genes are not suited for phylogenetic analysis and it is becoming increasingly apparent that the ompA gene locus is not congruent with the phylogeny of the C. pecorum genome. Using the recently sequenced C. pecorum genome sequence (E58), we analysed 10 genes, including ompA, to evaluate the use of ompA as a molecular marker in the study of koala C. pecorum genetic diversity. 相似文献386.
Reduced Pancreatic Exocrine Function and Organellar Disarray in a Canine Model of Acute Pancreatitis
Yuepeng Jin Yongyu Bai Qiang Li Pravin Avinash Bhugul Xince Huang Lewei Liu Liangliang Pan Haizhen Ni Bicheng Chen Hongwei Sun Qiyu Zhang Michael Hehir Mengtao Zhou 《PloS one》2016,11(2)
The aim of the present study was to investigate the pancreatic exocrine function in a canine model and to analyze the changes in organelles of pancreatic acinar cells during the early stage of acute pancreatitis (AP). AP was induced by retrograde injection of 5% sodium taurocholate (0.5 ml/kg) into the main pancreatic duct of dogs. The induction of AP resulted in serum hyperamylasemia and a marked reduction of amylase activity in the pancreatic fluid (PF). The pancreatic exocrine function was markedly decreased in subjects with AP compared with the control group. After the induction of AP, histological examination showed acinar cell edema, cytoplasmic vacuolization, fibroblasts infiltration, and inflammatory cell infiltration in the interstitium. Electron micrographs after the induction of AP revealed that most of the rough endoplasmic reticulum (RER) were dilated and that some of the ribosomes were no longer located on the RER. The mitochondria were swollen, with shortened and broken cristae. The present study demonstrated, in a canine model, a reduced volume of PF secretion with decreased enzyme secretion during the early stage of AP. Injury of mitochondria and dilatation and degranulation of RER may be responsible for the reduced exocrine function in AP. Furthermore, the present model and results may be useful for researching novel therapeutic measures in AP. 相似文献
387.
Singh Parminder Kaur Avinash Gupta Pooja Gill Sukhpal Singh Jyoti Kiran 《Cluster computing》2021,24(2):717-737
Cluster Computing - The elasticity characteristic of cloud services attracts application providers to deploy applications in a cloud environment. The scalability feature of cloud computing gives... 相似文献
388.
Hardik Dodia Avinash Vellore Sunder Yogen Borkar Pramod P. Wangikar 《Biotechnology and bioengineering》2023,120(10):2809-2826
Optimization and monitoring of bioprocesses requires the measurement of several process parameters and quality attributes. Mass spectrometry (MS)-based techniques such as those coupled to gas chromatography (GCMS) and liquid Chromatography (LCMS) enable the simultaneous measurement of hundreds of metabolites with high sensitivity. When applied to spent media, such metabolome analysis can help determine the sequence of substrate uptake and metabolite secretion, consequently facilitating better design of initial media and feeding strategy. Furthermore, the analysis of metabolite diversity and abundance from spent media will aid the determination of metabolic phases of the culture and the identification of metabolites as surrogate markers for product titer and quality. This review covers the recent advances in metabolomics analysis applied to the development and monitoring of bioprocesses. In this regard, we recommend a stepwise workflow and guidelines that a bioprocesses engineer can adopt to develop and optimize a fermentation process using spent media analysis. Finally, we show examples of how the use of MS can revolutionize the design and monitoring of bioprocesses. 相似文献
389.
Paithankar Jagdish Gopal Kudva Avinash Kundadka Raghu Shamprasad Varija Patil Rajashekhar K. 《Molecular biology reports》2020,47(4):2427-2436
Molecular Biology Reports - Exposure to ionizing radiation (IR) is a common phenomenon during medical diagnosis and treatment. IRs are deleterious because cellular exposure to IR can cause a series... 相似文献
390.
Avinash L. Puyad Gunturu Krishna Chaitanya Chetti Prabhakar Kotamarthi Bhanuprakash 《Journal of molecular modeling》2013,19(1):275-287
Semi-squaraines (SMSQ) are known as donor-acceptor (D-A) type molecules whereas squaraines (SQ), which differs from SMSQ by an extra donor group, are more or less biradicaloids in nature. The effect of the additional donor group in SQ, which changes the nature of the molecule, on geometrical and electronic structure are studied here and compared with the corresponding SMSQ. It is noticed from the geometrical parameters that, a strong resonance exists in SQ whereas disparity in carbon-carbon bond lengths of central C4 ring is seen in SMSQ dyes. The increasing and decreasing of antibonding interactions between central C4 ring and side donor groups cause destabilization of HOMO and stabilization of LUMO respectively in case of SQ compared to SMSQ molecules. This leads to decreasing the HOMO-LUMO gap and promotes biradicaloid character of SQ. The absorption maxima obtained by using TD-DFT method with BLYP, B3LYP, BHandHLYP, CAM-B3LYP and M06-2X functionals are not in good agreement with experimental results. On the other hand SAC-CI method gives better results for all the molecules. From this work we can evolve a design principle of these molecules which play a role as sensitizers in dye sensitized solar cells. 相似文献