排序方式: 共有79条查询结果,搜索用时 15 毫秒
41.
Aurelien Carlier Margo Cnockaert Linda Fehr Peter Vandamme Leo Eberl 《Systematic and applied microbiology》2017,40(1):11-21
Dioscorea sansibarensis is a monocot species of inedible yam native of tropical Africa and Madagascar. Leaves of D. sansibarensis feature prominent acumen glands that are densely colonized by bacteria. We describe here the isolation and characterization of bacteria from leaf glands of D. sansibarensis specimens obtained from various botanical gardens. Phylogenetic and phenotypic characterizations indicate that the isolates belong to a novel genus and species of the Alcaligenaceae family for which we propose the name Orrella dioscoreae gen. nov. sp. nov., with type strain LMG 29303T [=CIP 111009T]. Strains isolated from different plants show limited phylogenetic and phenotypic diversity, indicating specific and tight association of the bacteria with the host plant. The analysis of the draft genome of strain LMG 29303T reveals features consistent with a recently evolved plant-associated lifestyle. 相似文献
42.
Heparan sulfate alterations in extracellular matrix structures and fibroblast growth factor‐2 signaling impairment in the aged neurogenic niche
下载免费PDF全文
![点击此处可从《Journal of neurochemistry》网站下载免费的PDF全文](/ch/ext_images/free.gif)
43.
Circular dichroism spectrometry was used on oligogalacturonides (OGAs) and showed the existence of a calcium/sodium-induced conformational state that is intermediate between single-isolated chains and calcium-associated multimer chains. This conformation is interpreted as being egg box dimers. Using the 2F4 monoclonal antibody that specifically binds such an egg box dimer conformation of pectin, the stability of OGA dimers was investigated over a period of 24 hours. The extent to which egg box dimers were recognized by the antibody was dependent on the temperature and duration of preincubation of the OGA. This suggests a "maturation" process of the egg-box structure that consists in a progressive increase in the length of the junction sequences between two chains that slide along each other in order to form a maximum number of calcium bridges and dimer ends. The maturation of egg boxes induced both a significant increase in their binding to wall-associated kinase 1 (WAK1) and an increased extracellular alkalinization when applied to Arabidopsis thaliana cell suspensions. The chemical modification of the reducing end of the OGAs largely diminished their elicitating activity but did not hinder either dimerization or binding of these end-reduced egg boxes to WAK1. We conclude that there are at least two different perception systems for egg box dimers. One binds egg box junctions and the other binds egg box ends. The relevance of these results is discussed in terms of pectic signal perception and plant-pathogen interaction. 相似文献
44.
Marie-Claude Brulhart-Meynet Vincent Braunersreuther Jonas Brinck Fabrizio Montecucco Jean-Christophe Prost Aurelien Thomas Katia Galan Graziano Pelli Sarah Pedretti Nicolas Vuilleumier Fran?ois Mach Sandrine Lecour Richard W. James Miguel A. Frias 《PloS one》2015,10(3)
Background
New evidence shows that high density lipoproteins (HDL) have protective effects beyond their role in reverse cholesterol transport. Reconstituted HDL (rHDL) offer an attractive means of clinically exploiting these novel effects including cardioprotection against ischemia reperfusion injury (IRI). However, basic rHDL composition is limited to apolipoprotein AI (apoAI) and phospholipids; addition of bioactive compound may enhance its beneficial effects.Objective
The aim of this study was to investigate the role of rHDL in post-ischemic model, and to analyze the potential impact of sphingosine-1-phosphate (S1P) in rHDL formulations.Methods and Results
The impact of HDL on IRI was investigated using complementary in vivo, ex vivo and in vitro IRI models. Acute post-ischemic treatment with native HDL significantly reduced infarct size and cell death in the ex vivo, isolated heart (Langendorff) model and the in vivo model (-48%, p<0.01). Treatment with rHDL of basic formulation (apoAI + phospholipids) had a non-significant impact on cell death in vitro and on the infarct size ex vivo and in vivo. In contrast, rHDL containing S1P had a highly significant, protective influence ex vivo, and in vivo (-50%, p<0.01). This impact was comparable with the effects observed with native HDL. Pro-survival signaling proteins, Akt, STAT3 and ERK1/2 were similarly activated by HDL and rHDL containing S1P both in vitro (isolated cardiomyocytes) and in vivo.Conclusion
HDL afford protection against IRI in a clinically relevant model (post-ischemia). rHDL is significantly protective if supplemented with S1P. The protective impact of HDL appears to target directly the cardiomyocyte. 相似文献45.
46.
Thibault Varin Nicolas Saettel Jonathan Villain Aurelien Lesnard François Dauphin 《Journal of enzyme inhibition and medicinal chemistry》2013,28(5):593-603
5-Hydroxytryptamine subtype-4 (5-HT4) receptors have stimulated considerable interest amongst scientists and clinicians owing to their importance in neurophysiology and potential as therapeutic targets. A comparative analysis of hierarchical methods applied to data from one thousand 5-HT4 receptor–ligand binding interactions was carried out. The chemical structures were described as chemical and pharmacophore fingerprints. The definitions of indices, related to the quality of the hierarchies in being able to distinguish between active and inactive compounds, revealed two interesting hierarchies with the Unity (1 active cluster) and pharmacophore fingerprints (4 active clusters). The results of this study also showed the importance of correct choice of metrics as well as the effectiveness of a new alternative of the Ward clustering algorithm named Energy (Minimum E-Distance method). In parallel, the relationship between these classifications and a previously defined 3D 5-HT4 antagonist pharmacophore was established. 相似文献
47.
Valentina Cappelletti Thomas Hauser Ilaria Piazza Monika Pepelnjak Liliana Malinovska Tobias Fuhrer Yaozong Li Christian Dörig Paul Boersema Ludovic Gillet Jan Grossbach Aurelien Dugourd Julio Saez-Rodriguez Andreas Beyer Nicola Zamboni Amedeo Caflisch Natalie de Souza Paola Picotti 《Cell》2021,184(2):545-559.e22
- Download : Download high-res image (211KB)
- Download : Download full-size image
48.
Julia T. Vilstrup Andaine Seguin-Orlando Mathias Stiller Aurelien Ginolhac Maanasa Raghavan Sandra C. A. Nielsen Jacobo Weinstock Duane Froese Sergei K. Vasiliev Nikolai D. Ovodov Joel Clary Kristofer M. Helgen Robert C. Fleischer Alan Cooper Beth Shapiro Ludovic Orlando 《PloS one》2013,8(2)
The genus Equus is richly represented in the fossil record, yet our understanding of taxonomic relationships within this genus remains limited. To estimate the phylogenetic relationships among modern horses, zebras, asses and donkeys, we generated the first data set including complete mitochondrial sequences from all seven extant lineages within the genus Equus. Bayesian and Maximum Likelihood phylogenetic inference confirms that zebras are monophyletic within the genus, and the Plains and Grevy’s zebras form a well-supported monophyletic group. Using ancient DNA techniques, we further characterize the complete mitochondrial genomes of three extinct equid lineages (the New World stilt-legged horses, NWSLH; the subgenus Sussemionus; and the Quagga, Equus quagga quagga). Comparisons with extant taxa confirm the NWSLH as being part of the caballines, and the Quagga and Plains zebras as being conspecific. However, the evolutionary relationships among the non-caballine lineages, including the now-extinct subgenus Sussemionus, remain unresolved, most likely due to extremely rapid radiation within this group. The closest living outgroups (rhinos and tapirs) were found to be too phylogenetically distant to calibrate reliable molecular clocks. Additional mitochondrial genome sequence data, including radiocarbon dated ancient equids, will be required before revisiting the exact timing of the lineage radiation leading up to modern equids, which for now were found to have possibly shared a common ancestor as far as up to 4 Million years ago (Mya). 相似文献
49.
Mathieu Vanhoute Renato Froidevaux Aurelien Vanvlassenbroeck Didier Lecouturier Pascal Dhulster Didier Guillochon 《Journal of chromatography. B, Analytical technologies in the biomedical and life sciences》2009,877(16-17):1683-1688
The ion-pair concept was applied on complex haemoglobin hydrolysates to extract two opioid peptides, LVV-haemorphin-7 and VV-haemorphin-7, in an aqueous/octan-1-ol micro-extraction system in the presence of alkyl-sulfonic acid as a surfactant agent and in relation to the haemorphin physico-chemical properties (charge, hydrophobicity). The effect of combined alkyl chain length/aqueous phase pH and the haem behaviour during the extraction, on the haemorphin recovery yield and enrichment has been determined. It has proved that transport over the organic phase is mediated by the alkyl-sulfonic acids, whatever be the aqueous phase pH. However, increasing both the alkyl chain length and the pH in the aqueous phase shows an haemorphin enrichment ratio increase but a recovery decrease of the extracted opioid peptides in the organic phase. Therefore, the best conditions to extract LVVh-7 and VVh-7 are the use of the octane-sulfonic acid at aqueous phase pH of 5 or 7 and the octane or the heptane-sulfonic acid with an aqueous phase pH of 5 or 7 respectively. In these conditions, a partition coefficient of 1.64 and 1.60 respectively for LVVh-7 and VVh-7 are obtained and represent about 40 times that acquired without agent. 相似文献
50.
Julie Cahu Aurelien Olichon Christian Hentrich Henry Schek Jovana Drinjakovic Cunjie Zhang Amanda Doherty-Kirby Gilles Lajoie Thomas Surrey 《PloS one》2008,3(12)