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排序方式: 共有236条查询结果,搜索用时 46 毫秒
161.
Pancorbo PL de Pablo MA Ortega E Puertollano MA Gallego AM Alvarez de Cienfuegos G 《Current microbiology》2001,43(3):209-214
Campylobacter jejuni has been reported to produce different toxins that may modulate the immune response in both animals and humans. The effect
of C. jejuni enterotoxin on the immune response was investigated in two groups of Balb/c mice. One of them was inoculated intraperitoneally
with 1010 colony forming units (CFU) of an enterotoxigenic strain (CCUG 7580), and the second one with a non-enterotoxigenic strain
(CCUG 7440). The number of polymorphonuclear (PMN) cells from spleen increased in both enterotoxigenic and non-enterotoxigenic
strains as a consequence of C. jejuni infection. Notwithstanding, lymphocyte proliferation stimulated by lipopolysaccharide (LPS) was increased by both enterotoxigenic
and non-enterotoxigenic strains. Interleukin-2 (IL-2) production from splenic cells was increased significantly by infection
with the enterotoxigenic strain. Both enterotoxigenic and non-enterotoxigenic strains reduced the splenic response to sheep
erythrocytes; the response was significantly suppressed for immunoglobulin M (Ig M) and for immunoglobulin G (Ig G) synthesis.
These results suggest that C. jejuni is able to modify some components of the immune response in mice, and also that the enterotoxigenic strain has more immunomodulating
activity than the non-enterotoxigenic strain.
Received: 4 December 2000/Accepted: 6 February 2001 相似文献
162.
Lartigue A Drouot L Jouen F Charlionet R Tron F Gilbert D 《Arthritis research & therapy》2005,7(6):R1394-R1403
We showed previously that nucleophosmin (NPM), a nucleolar phosphoprotein, is recognized by sera from (NZW x BXSB)F1 (WB) mice, a model of systemic lupus erythematosus (SLE) and anti-phospholipid syndrome. In the present study we analysed the prevalence and kinetics of anti-NPM autoantibodies in WB mice by a solid-phase ELISA with recombinant human (rh) NPM as the antigen and showed that most male WB mouse sera had anti-NPM antibodies that were responsible for their indirect immunofluorescence staining pattern on Hep-2 cells. Anti-NPM antibodies were significantly associated with anti-cardiolipin (aCL) antibodies. This antibody profile mirrored that observed in certain human SLE sera because anti-NPM antibodies were detected in 28% of the sera from patients with SLE and were similarly associated with aCL antibodies. The demonstration that rhNPM bound to cardiolipin (CL) in vitro and increased the CL-binding activity of a WB-derived aCL monoclonal antibody indicates that NPM can interact with CL to form SLE-related immunogenic particles that might be responsible for the concomitant production of anti-NPM and aCL antibodies. 相似文献
163.
Piacente S Camargo EE Zampelli A Gracioso JS Souza Brito AR Pizza C Vilegas W 《Zeitschrift für Naturforschung. C, Journal of biosciences》2002,57(11-12):983-985
The infusion of the aerial parts of Turnera diffusa was phytochemically examined. Chromatographic procedures led to the isolation of a new flavone glycoside, five known flavonoids and p-arbutin. Structures were determined by 1D- and 2D NMR experiments, as well as ES-MS and UV spectra. 相似文献
164.
165.
166.
The simultaneous hatching of Daphnia resting eggs from a number of different lakes and ponds using one set of hatching cues can be difficult to achieve as environmental conditions differ considerably between water bodies. Therefore, optimal hatching conditions for ephippial eggs originating from shallow temporary waters may differ from those found in large lakes. The aim of our study was to compare the optimal thermal conditions for hatching ephippial eggs of Daphnia found in permanent lakes and those from shallow temporary ponds. We used ephippial eggs of Daphnia from the longispina species complex originating from two temporary city ponds and two deep lakes in Poland. The ephippia were protected against overheating at all stages of the field and laboratory work to prevent activation or killing of the eggs. After a refractory period (imposed storage in cool and dark conditions), ephippia were incubated at six different temperatures (6, 9, 12, 15, 18 and 21 °C) under a 16:8 L:D light regime. Our results indicate that hatching of resting eggs of Daphnia that inhabit lakes or ponds may require different thermal conditions. The hatching success of ephippial eggs originating from temporary waters was relatively high (30–56%) at all tested incubation temperatures, while for the ephippial eggs from the deep lakes it was lower (7–37%) and inversely related to water temperature. The divergent hatching responses of the ephippial eggs originating from temporary pools and lakes may reflect the typical thermal conditions during hatching in their native habitats. While in the deep lakes of the temperate zone Daphnia hatching typically occurs during the low water temperatures of early spring, in shallow ponds Daphnia hatching may occur throughout the year at varying water temperatures, from a few to over a dozen degrees Celsius. 相似文献
167.
Petrarca C Casalino B von Mensdorff-Pouilly S Rughetti A Rahimi H Scambia G Hilgers J Frati L Nuti M 《Cancer immunology, immunotherapy : CII》1999,47(5):272-277
The humoral immune response against a tumour-associated antigen, polymorphic epithelial mucin (PEM, MUC1) in cancer patients
was studied by isolating specific B cells primed for the antigen. Human B lymphocytes from tumour-draining lymph nodes, obtained
from 12 patients with epithelial cancers, were immunoselected with magnetic beads coated with a 60mer synthetic peptide corresponding
to three tandem repeats of the protein core of the MUC1 antigen. Short-term cultures of B cells were established utilizing
interleukin-10 (IL-10), IL-4 and monoclonal antibody anti-CD40, and were maintained for a maximum of 3␣weeks. B cell culture
supernatants contained human anti-MUC1 antibodies, as detected by enzyme-linked immunosorbent assay, in 6/12 of the patients
tested. Five of these patients, all with early-stage cancer, also had high levels of circulating anti-MUC1 IgM antibodies
in the serum. A significant correlation was found (two-tailed P = 0.041) between the presence of circulating anti-MUC1 antibodies and the ability to isolate PEM-specific B cells from tumour-draining
lymph nodes. The technique proposed provides a useful method for the analysis of natural immunity against defined tumour antigens.
Received: 30 June 1998 / Accepted: 5 October 1998 相似文献
168.
Epifano F Genovese S Sosa S Tubaro A Curini M 《Bioorganic & medicinal chemistry letters》2007,17(20):5709-5714
Different esters of 3-(4'-geranyloxy-3'-methoxyphenyl)-2-trans propenoic acid (1), an anti-inflammatory principle of Acronychia baueri Schott (Rutaceae), were synthesized. Their topical anti-inflammatory activity was evaluated using the Croton oil ear test in mice as a model of acute inflammation. The activity of the paracetamol, guaiacol and hydroquinone esters of (1) was higher than that of the parent compound, being similar to that exerted by indomethacin, used as reference drug. 相似文献
169.
Beata Krawczyk Anna Śledzińska Agnieszka Piekarska Andrzej Hellmann Józef Kur 《Journal of applied genetics》2017,58(3):415-418
In patients with haematological malignancies, the bowel remains the main source of Escherichia coli bloodstream infections. We present the clinical example of recurrent bowel-blood translocations of E. coli with the unique virulence characteristics in a 55-year-old male with the diagnosis of acute myeloid leukaemia. The virulent factors profile of examined strains confirmed that the co-existence of genes papC, sfa, usp and cnf1, encoding virulence factors, predisposes E. coli to translocation from the gastrointestinal tract to the vascular bed. The close cooperation between haematologists and microbiologists is essential to improve the outcome of patients colonised with highly pathogenic strains. 相似文献
170.
Kuliszkiewicz-Janus M Tuz MA Kiełbiński M Baczyński S Jaźwiec B Sladowska H 《Cellular & molecular biology letters》2008,13(1):58-66
The aim of this investigation was to evaluate the changes in PAF concentrations in the plasma, PBMC and BMMC of patients with
acute lymphoblastic leukemia (ALL) and acute myeloblastic leukemia (AML). The plasma was from 23 healthy volunteers (HV) and
44 patients with AL (16 ALL, 28 AML). The PBMC were from 15 HV and 55 patients with AL (18 ALL, 37 AML), and the BMMC from
40 patients with AL (11 ALL, 29 AML). Methanol-chloroform phospholipid extraction from 60 × 106 cells (PBMC or BMMC) was performed according to a modified version of Folch’s method. 31P MRS data was obtained on an AMX 300 Bruker spectrometer (7.05 T). The PAF concentration in the plasma of the patients with
ALL or AML was lower than that for the healthy volunteers. The PAF concentration in the plasma of the patients with ALL did
not differ significantly from that of the patients with AML. In the case of both the PBMC and BMMC, the PAF concentration
was significantly diminished in patients with ALL relative to the concentration for those with AML and for the healthy volunteers.
No differences were observed in the PAF concentrations for the AML patients and the healthy volunteers. 相似文献