全文获取类型
收费全文 | 237篇 |
免费 | 15篇 |
专业分类
252篇 |
出版年
2016年 | 8篇 |
2015年 | 4篇 |
2014年 | 12篇 |
2013年 | 11篇 |
2012年 | 6篇 |
2011年 | 15篇 |
2010年 | 7篇 |
2009年 | 7篇 |
2008年 | 6篇 |
2007年 | 7篇 |
2006年 | 9篇 |
2005年 | 3篇 |
2004年 | 7篇 |
2003年 | 8篇 |
2001年 | 3篇 |
2000年 | 8篇 |
1999年 | 2篇 |
1998年 | 2篇 |
1996年 | 3篇 |
1993年 | 3篇 |
1992年 | 3篇 |
1991年 | 3篇 |
1990年 | 5篇 |
1989年 | 8篇 |
1988年 | 8篇 |
1987年 | 4篇 |
1986年 | 6篇 |
1985年 | 2篇 |
1983年 | 4篇 |
1982年 | 2篇 |
1977年 | 2篇 |
1974年 | 4篇 |
1972年 | 4篇 |
1971年 | 2篇 |
1970年 | 3篇 |
1969年 | 2篇 |
1968年 | 2篇 |
1940年 | 2篇 |
1937年 | 2篇 |
1936年 | 2篇 |
1929年 | 2篇 |
1928年 | 3篇 |
1927年 | 3篇 |
1925年 | 2篇 |
1921年 | 2篇 |
1920年 | 3篇 |
1918年 | 2篇 |
1917年 | 2篇 |
1914年 | 2篇 |
1908年 | 3篇 |
排序方式: 共有252条查询结果,搜索用时 0 毫秒
191.
Sorbitol dehydrogenase from human liver was purified to homogeneity by affinity chromatography on immobilized triazine dyes, conventional cation-exchange chromatography, and high-performance liquid chromatography. The major form is a tetrameric, NAD-specific enzyme containing one zinc atom per subunit. Human liver sorbitol dehydrogenase oxidizes neither ethanol nor other primary alcohols. It catalyzes the oxidation of a secondary alcohol group of polyol substrates such as sorbitol, xylitol, or L-threitol. However, the substrate specificity of human liver sorbitol dehydrogenase is broader than that of the liver enzymes of other sources. The present report describes the stereospecific oxidation of (2R,3R)-2,3-butanediol, indicating a more general function of sorbitol dehydrogenase in the metabolism of secondary alcohols. Thus, the enzyme complements the substrate specificities covered by the three classes of human liver alcohol dehydrogenase. 相似文献
192.
193.
194.
195.
A. G. Auld 《BMJ (Clinical research ed.)》1914,1(2785):1060-1062
196.
197.
198.
199.
R Bicknell A Schaeffer D S Auld J F Riordan R Monnanni I Bertini 《Biochemical and biophysical research communications》1985,133(2):787-793
Proteases in preparations of carboxypeptidase A progressively inactivate solutions of the apoenzyme but not the metal-containing enzyme. Free amino acids generated by proteolysis interfere with spectral studies after reconstituting the apoenzyme with cobalt. Purification by affinity chromatography eliminates this effect. Affinity-purified apoenzyme is susceptible to digestion with chymotrypsin but the metalloenzyme is not. 相似文献
200.
Rapid-scanning stopped-flow spectroscopy (425-700 nm) has been used to study spectral changes in cobalt(II)-substituted Bacillus cereus beta-lactamase II during the binding and hydrolysis of benzylpenicillin. The experiments were carried out in aqueous solution over a temperature range of 3-20 degrees C. Three metallointermediates have been characterized by their visible absorption spectra. Two of them have visible absorption spectra identical with the intermediates ES1 and ES2 previously observed at subzero temperatures in a mixed aqueous/organic solvent [Bicknell, R., & Waley, S.G. (1985) Biochemistry 24, 6876-6887]. In addition, the branched kinetic pathway observed with the zinc(II) and cobalt(II) beta-lactamase II at subzero temperatures has been shown to occur with the cobalt(II)-substituted enzyme in aqueous solution at above-zero temperatures; thus, at pH 6.0 and 3 degrees C, the rate and equilibrium constants are readily determined for the reaction scheme: (Formula: see text). A third transient intermediate (called ES*) was found to precede ES1 in the pre-steady-state time period. The identity of the intermediates formed in aqueous solution with those previously observed in the cryostudy confirms that the mechanism is not changed either by the presence of an organic cosolvent or by subzero temperatures. Further characterization of ES1 and the steady-state intermediate ES2 at subzero temperatures, where their lifetime may be extended for up to several hours, has involved circular and magnetic circular dichroic studies. The magnetic circular dichroic spectra identify changes in the coordination sphere of the active-site metal during catalysis.(ABSTRACT TRUNCATED AT 250 WORDS) 相似文献