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排序方式: 共有146条查询结果,搜索用时 15 毫秒
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Laurine Buscara Fatemeh Montazer-Torbati Sead Chadi Aurélie Auguste Johann Laubier Anne-Amandine Chassot Lauriane Renault Bruno Passet José Costa Maëlle Pannetier Marthe Vilotte Marie-Christine Chaboissier Jean-Luc Vilotte Eric Pailhoux Fabienne Le Provost 《Transgenic research》2009,18(4):649-654
RSPO1 is a newly discovered gene involved in sex differentiation. Two goat BAC clones encompassing the RSPO1 gene (gRSPO1) were injected into mouse oocytes and several transgenic lines derived. Both clones induced gRSPO1 over-expression in various tissues, including male and female gonads, with no obvious phenotype and normal sex-ratios. Introgression
of the gRSPO1 transgene into a mouse RSPO1 knockout genotype resulted in the rescue of the fertility and the disappearance of the masculinized gonadic features of the
females, demonstrating the functionality of the goat protein in a mouse context. On the contrary, over-expression of gRSPO1 within a mSRY or a gSRY-XX genotypes did not interfere with the SRY-induced male phenotype.
Laurine Buscara and Fatemeh Montazer-Torbati contributed equally to this work. 相似文献
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Thierry Christophe Mary Jackson Hee Kyoung Jeon Denis Fenistein Monica Contreras-Dominguez Jaeseung Kim Auguste Genovesio Jean-Philippe Carralot Fanny Ewann Eun Hye Kim Sae Yeon Lee Sunhee Kang Min Jung Seo Eun Jung Park Henrieta ?kovierová Ha Pham Giovanna Riccardi Ji Youn Nam Laurent Marsollier Marie Kempf Marie-Laure Joly-Guillou Taegwon Oh Won Kyung Shin Zaesung No Ulf Nehrbass Roland Brosch Stewart T. Cole Priscille Brodin 《PLoS pathogens》2009,5(10)
A critical feature of Mycobacterium tuberculosis, the causative agent of human tuberculosis (TB), is its ability to survive and multiply within macrophages, making these host cells an ideal niche for persisting microbes. Killing the intracellular tubercle bacilli is a key requirement for efficient tuberculosis treatment, yet identifying potent inhibitors has been hampered by labor-intensive techniques and lack of validated targets. Here, we present the development of a phenotypic cell-based assay that uses automated confocal fluorescence microscopy for high throughput screening of chemicals that interfere with the replication of M. tuberculosis within macrophages. Screening a library of 57,000 small molecules led to the identification of 135 active compounds with potent intracellular anti-mycobacterial efficacy and no host cell toxicity. Among these, the dinitrobenzamide derivatives (DNB) showed high activity against M. tuberculosis, including extensively drug resistant (XDR) strains. More importantly, we demonstrate that incubation of M. tuberculosis with DNB inhibited the formation of both lipoarabinomannan and arabinogalactan, attributable to the inhibition of decaprenyl-phospho-arabinose synthesis catalyzed by the decaprenyl-phosphoribose 2′ epimerase DprE1/DprE2. Inhibition of this new target will likely contribute to new therapeutic solutions against emerging XDR-TB. Beyond validating the high throughput/content screening approach, our results open new avenues for finding the next generation of antimicrobials. 相似文献
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Taillades J Collet H Garrel L Beuzelin I Boiteau L Choukroun H Commeyras A 《Journal of molecular evolution》1999,48(6):638-645
α-N-Carbamoyl amino acid (CAA), whose conditions of formation in a prebiotic hydrosphere have been described previously (Taillades
et al. 1998), could have been an important intermediate in prebiotic peptide synthesis through reaction with atmospheric NO
x
. Nitrosation of solid CAA (glycine or valine derivative) by a 4/1 NO/O2 gaseous mixture (1 atm) yields N-carboxyanhydride (NCA) quantitatively in less than 1 h at room temperature. The crude solid NCA undergoes quantitative oligomerization
(from trimer to nonamer under the conditions we used) when treated with a (bi)carbonate aqueous buffer at pH 9. We therefore
suggest that part of the prebiotic amino acid activation/polymerization process may have taken place in a dry phase (``drying-lagoon'
scenario).
Received: 23 June 1998 / Accepted: 7 December 1998 相似文献
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U. S Dinish Flavien Beffara Georges Humbert Jean‐Louis Auguste Malini Olivo 《Journal of biophotonics》2019,12(11)
The tremendous enhancement factors that surface‐enhanced Raman scattering (SERS) possesses coupled with the flexibility of photonic crystal fibers (PCFs) pave the way to a new generation of ultrasensitive biosensors. Thanks to the unique structure of PCFs, which allows direct incorporation of an analyte into the axially aligned air channels, interaction between the analyte and excitation light could be increased many folds leading to flexible, reliable and sensitive probes that can be used in preclinical or clinical biosensing. SERS‐active PCF probes provide unique opportunity to develop an opto‐fluidic liquid biopsy needle sensor that enables one‐step integrated sample collection and testing for disease diagnosis. Specificity being a key parameter to biosensors, the PCF inside the biopsy needle could be functionalized with targeting moieties to detect specific biomarkers. In this review article, we present some of the most promising recent biosensors based on PCFs including hollow‐core PCFs, suspended‐core PCFs and side‐channel PCFs. We provide a wide range of applications of such platform using Raman spectroscopy, label free SERS or labeled SERS detection and analyze some of the main challenges to be addressed for translating it to a clinically viable next generation sensitive biopsy needle sensing probe. 相似文献
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