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211.
A. Gallois J. C. Audran M. Burrus 《TAG. Theoretical and applied genetics. Theoretische und angewandte Genetik》1998,97(1-2):211-219
We assessed the genetic relationships between members of the Fagaceae family by RAPDs in order to better ascertain the taxonomic status of a very particular population of Fagus sylvatica, the ‘tortuosa’ variety. Intra- and inter-population Nei and Li’s mean genetic distances were compared, and the genetic relationships
between individuals were clarified on dendrograms by the Neighbor joining method. RAPD analysis was first conducted on three
species from three genera, Quercus petraea, Castanea sativa, and Fagus sylvatica, in order to develop an efficient RAPD protocol. The variety level was then studied, and a general tendency of the individuals
to cluster by variety was observed. Individuals also clustered by geographic locations, but the genetic distances between
populations were not correlated to the distances between sites. Finally, we compared the common beech and ‘tortuosa’ varieties
from two different locations, Verzy and Süntel. Both populations from one location were closer than the same variety from
two sites. This last result is in agreement with those previously obtained with isozymes. Hypotheses concerning the origin
of the ‘tortuosa’ variety are discussed.
Received: 9 January 1998 / Accepted: 25 February 1998 相似文献
212.
213.
Developing taste buds in the anterior mandibular floor of perihatching
chicks were studied by high voltage electron microscopic autoradiography in
order to identify proliferating gemmal cell types. Montaged profiles of 29
taste buds in five cases euthanized between embryonic day 21 and
posthatching day 2 were analyzed after a single [3H]thymidine injection
administered on embryonic day 16, 17 or 18. Results showed that dark cells
comprised 55% of identified (n = 900 cells) and 62% of labeled (n = 568
cells) gemmal cells as compared with light, intermediate, basal or
perigemmal bud cells. Dark cells had both a greater (P < 0.05) number of
labeled cells and a greater amount of label (grains/nucleus) than the other
four bud cell types, irrespective of injection day. The nuclear area
(micron 2) of dark cells was not significantly larger (P > 0.05) than
that of the other gemmal cell types and therefore cannot account for the
greater amount for label in the dark cells. Interestingly, only dark cells
showed a positive correlation (P < 0.003) between amount of label and
nuclear area. Results suggest that, during the perihatching period of
robust cell proliferation, dividing dark cells may give rise primarily, but
not exclusively, to dark cell progeny.
相似文献
214.